420400 | Anti-KDEL Mouse mAb (10C3)

420400
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ca, Dr, Gp, Ht, H, Mk, M, Porcine, Rb, R, Sh, Yeast M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      420400-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes the Grp78 (BiP) and Grp94 proteins.
          Catalogue Number420400
          Brand Family Calbiochem®
          Application Data
          Detection of KDEL by immunoblotting. Samples: Recombinant Grp78 (BiP) protein (lane 1), whole cell lysate RK-13 cells (lane 2), mouse liver microsomes (lane 3), rat liver microsomes (lane 4), and whole cell lysate from heat shocked HeLa cells (lane 5). Primary antibody: Anti-KDEL Mouse mAb (10C3) (Cat. No. 420400) (1:1000). Detection: chemiluminescence.
          References
          ReferencesKuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990.
          Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
          Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
          Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.
          Product Information
          FormLiquid
          FormulationIn PBS, 50% glycerol.
          Positive controlRK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Application NotesImmunoblotting (4 µg/ml, colorimetric)
          Immunocytochemistry (5 µg/ml)
          Immunoprecipitation (5 µg/ml)
          Application CommentsAlso recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH
          ImmunogenRat
          Clone10C3
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Canine
          • Drosophila
          • Guinea Pig
          • Hamster
          • Human
          • Monkey
          • Mouse
          • Porcine
          • Rabbit
          • Rat
          • Sheep
          • Yeast
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Highly Toxic
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          420400

          References

          Reference overview
          Kuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990.
          Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
          Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
          Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-August-2007 RFH
          ApplicationImmunoblotting (4 µg/ml, colorimetric)
          Immunocytochemistry (5 µg/ml)
          Immunoprecipitation (5 µg/ml)
          Application Data
          Detection of KDEL by immunoblotting. Samples: Recombinant Grp78 (BiP) protein (lane 1), whole cell lysate RK-13 cells (lane 2), mouse liver microsomes (lane 3), rat liver microsomes (lane 4), and whole cell lysate from heat shocked HeLa cells (lane 5). Primary antibody: Anti-KDEL Mouse mAb (10C3) (Cat. No. 420400) (1:1000). Detection: chemiluminescence.
          DescriptionProtein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.
          BackgroundSeveral resident proteins in the ER are involved in the proper assembly and folding of newly synthesized proteins. These include the glucose-regulated protein 94 (Grp94), BiP, which functions in protein oligomerization, and protein disulfide isomerase (PDI), which catalyzes the rearrangement of the disulfide bond. Grp94, BiP, and PDI share a carboxy-terminal sequence (Lys-Asp-Glu-Leu) with other resident ER proteins. The KDEL sequence is sufficient for the retention of a protein in the ER.
          HostMouse
          Immunogen speciesRat
          Immunogena synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH
          Clone10C3
          IsotypeIgG2a
          SpeciesDrosophila, canine, guinea pig, hamster, human, monkey, mouse, porcine, rabbit, rat, sheep, yeast
          Positive controlRK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
          FormLiquid
          FormulationIn PBS, 50% glycerol.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsAlso recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Highly Toxic
          ReferencesKuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990.
          Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
          Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
          Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.