ABE1859 | Anti-KAP-1/TRIM28 Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IP, ICC Rb Serum Polyclonal Antibody
      Catalogue NumberABE1859
      DescriptionAnti-KAP-1/TRIM28 Antibody
      Alternate Names
      • Transcription intermediary factor 1-beta
      • TIF1-beta
      • E3 SUMO-protein ligase TRIM28
      • KRAB-associated protein 1
      • KAP-1
      • KRAB-interacting protein 1
      • KRIP-1
      • Nuclear corepressor KAP-1
      • RING finger protein 96
      • Tripartite motif-containing protein 28
      • KAP-1/TRIM28
      Background InformationTranscription intermediary factor 1-beta (UniProt Q13263; also known as E3 SUMO-protein ligase TRIM28, KAP-1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, Protein phosphatase 1 regulatory subunit 157, RING finger protein 96, TIF1-beta, Transcriptional intermediary factor 1-beta, Tripartite motif-containing 28, Tripartite motif-containing protein 28) is encoded by the TRIM28 (also known as KAP1, PPP1R157, RNF96, TF1B, TIF1B) gene (Gene ID 10155) in human.
      Kruppel-associated box-/KRAB-associated protein 1 (KAP-1) is a transcriptional co-repressor that selectively targets the KRAB domain of zinc finger protein-based transcription factors with its N-terminal tripartite RBCC (RING, b-box, coiled-coil) domain. KAP-1 uses its C-terminal PxVxL motif and the PHD-Bromo domain to recruit chromatin modifiers, such as heterochromatin protein 1 (HP-1), Histone-lysine N-methyltransferase SETDB1, nucleosome remodeling and deacetylation (NuRD) complex, and histone deacetylases (HDACs), for transcriptional repression. KAP-1 also plays an essential role in the DNA damage response (DDR). Upon DNA damage, KAP-1 is rapidly phosphorylated by Ataxia telangiectasia mutated (ATM) on Ser824, causing chromatin relaxation to facilitate DNA repair. In addition, SIRT1-mediated KAP-1 deacetylation is reported to stabilize KAP1 and 53BP1 interaction, resulting in an enhancement of NHEJ-mediated repair efficiency in a KAP-1 pSer824-independent manner.
      Product Information
      PresentationRabbit polyclonal antibody in serum with 0.05% sodium azide.
      ApplicationAnti-KAP-1/TRIM28 Antibody is an antibody against KAP-1/TRIM28 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected the endogenous KAP-1/Trim28 only in the immunoprecipitats of exogenously expressed FLAG-SirT1 constructs that contain the KAP-1/Trim28-binding domain (a.a. 143-213) using various transfected HEK293T cells (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
      Western Blotting Analysis: A representative lot detected similar level of endogenous KAP-1/Trim28 in HEK293T cells regardless of ionizing radiation treatment or shRNA-mediated SirT1 downregulation (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
      Western Blotting Analysis: A representative lot detected KAP-1/Trim28 in whole cell lysates, but not in BAP1, FoxK1, or FoxK2 immunoprecipitate (Okino, Y., et al. (2015). J. Biol. Chem. 16;290(3):1580-1591).
      Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated KAP-1/Trim28 from HEK293T cell lysates (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
      Immunocytochemistry Analysis: An 1:1000 dilution from a representative lot detected KAP-1/Trim28 nuclear localization in U2OS cells (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
      Immunoprecipitation Analysis: A representative lot co-immunoprecipitated SirT1 with KAP-1/Trim28 from HEK293T cell lysates (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
      Western Blotting Analysis: An 1:1000 dilution from a representative lot detected KAP-1/Trim28 in mouse embryonic fibroblast (MEF) lysate, as well as downregulated KAP-1/Trim28 in lysates from KAP1 siRNA-treated HEK293T cells (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the Bromo domain of human KAP-1/TRIM28.
      EpitopeBromo domain
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityExpected to react with both spliced isoforms.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TRIM28
      • KAP1
      • RNF96
      • TIF1B
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~110 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected KAP-1/TRIM28 in 10 µg of HEK293T cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-KAP-1/TRIM28 -Q2629029 Q2629029


      Reference overviewPub Med ID
      BRCA1-associated protein 1 (BAP1) deubiquitinase antagonizes the ubiquitin-mediated activation of FoxK2 target genes.
      Okino, Y; Machida, Y; Frankland-Searby, S; Machida, YJ
      The Journal of biological chemistry  290  1580-91  2015

      Show Abstract
      25451922 25451922
      KAP1 Deacetylation by SIRT1 Promotes Non-Homologous End-Joining Repair.
      Lin, YH; Yuan, J; Pei, H; Liu, T; Ann, DK; Lou, Z
      PloS one  10  e0123935  2015

      Show Abstract
      25905708 25905708

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      Life Science Research > Antibodies and Assays > Primary Antibodies