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MABE326 | Anti-JARID1D/KDM5D Antibody, clone 7F10.4

100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC M Purified Monoclonal Antibody
      Catalogue NumberMABE326
      DescriptionAnti-JARID1D/KDM5D Antibody, clone 7F10.4
      Alternate Names
      • JARID1D/KDM5D
      • Lysine-specific demethylase 5D
      • Histocompatibility Y antigen
      • H-Y
      • Histone demethylase JARID1D
      • Jumonji/ARID domain-containing protein 1D
      • Protein SmcY
      Background InformationLysine-specific demethylase 5D (UniProt Q9BY66; also known as H-Y, Histone demethylase JARID1D, Histocompatibility Y antigen, Human homolog of selected mouse cDNA on Y, Jumonji/ARID domain-containing protein 1D, Jumonji AT rich interactive domain 1D (RBP2-like), Protein SmcY, SMC homolog Y chromosome, Smcy homolog Y-linked) is encoded by the KDM5D (also known as HY, HYA, JARID1D, KIAA0234, SMCY) gene (Gene ID 8284) in human. JARID1D/KDM5D is a histone demethylase that specifically catalyzes the demethylation of tri- and di-methylated Lys4 of histone H3 (H3K4m3 and H3K4m2), but not methylated H3K9, H3K27, H3K36, H3K79, H4K20, or monomethylated H3K4. The KDM5D gene is mapped to the azoospermia factor (AZF) region known to be involved in spermatogenesis.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-JARID1D Antibody, clone 7F10.4 is validated for use in Western Blotting and Immunocytochemistry for the detection of KDM5D .
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a representative lot detected JARID1D/KDM5D in HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human JARID1D/KDM5D.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 7F10.4 is expected to react with all three spliced JARID1D/KDM5D isoforms.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KDM5D
      • HY
      • HYA
      • JARID1D
      • KIAA0234
      • SMCY
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~200 kDa observed. Isoform 1 (174.1 kDa calculated); Isoform 2 (167.4 kDa calculated); Isoform 3 (177.6 kDa calculated). Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2OS cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected JARID1D/KDM5D in 10 µg of U2OS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies