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MABT128 | Anti-JAM-A Antibody, clone BV11

MABT128
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M FC, ICC, IP R Purified Monoclonal Antibody
      Description
      Catalogue NumberMABT128
      DescriptionAnti-JAM-A Antibody, clone BV11
      Alternate Names
      • JAM
      • JAM-1
      • JAM-A
      • Ly06
      • ESTM33
      • AA638916
      • 9130004G24
      • F11r
      • Junctional adhesion molecule 1
      • CD321
      Background InformationJunctional adhesion molecule A (JAM-A) is found in endothelial and epithelial tight junctions and is involved in the inflammatory enlistment of mononuclear cells containing LFA-1. JAM-A is linked to atherogenesis, platelet activation, transmigration of leukocytes and angiogenesis. JAM-A is also found to be important in the development of many different organ systems, and is mostly expressed in the epithelial parts and the embryonic vasculature. JAM-A is indicated to play a role in epithelial permeability, inflammation and proliferation in the intestine, aiding in homeostasis.
      References
      Product Information
      FormatPurified
      Control
      • bEnd.3 cells
      PresentationPurified rat monocolonal IgG2bκ in buffer containing PBS and no preservatives.
      Applications
      ApplicationDetect JAM-A using this Anti-JAM-A Antibody, clone BV11 validated for use in FC, IC & IP.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot was used by an independent laboratory in cell extracts from Mouse H5V and PDV cells, and in COS cultures transfected with full length cDNA of mouse JAM-A (Martin-Padura, I., et al. (1998). J Cell Biol. 142(1):117-127).

      Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in cultured epithelial cells, cremaster muscles, and Schwann Cells (Martin-Padura, I., et al. (1998). J Cell Biol. 142(1):117-127).
      Biological Information
      ImmunogenH5V murine heart capillary endothelial cells.
      CloneBV11
      HostRat
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • F11r
      • Jam1
      • Jcam
      • Jcam1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.

      SUBUNIT STRUCTURE: Interacts with the ninth PDZ domain of MPDZ. Interacts with the first PDZ domain of PARD3. The association between PARD3 and PARD6B probably disrupts this interaction. Interacts with the orthoreovirus sigma-1 capsid protein.

      SUBCELLULAR LOCATION: Cell junction › tight junction. Cell membrane; Single-pass type I membrane protein. Note: Localized at tight junctions of both epithelial and endothelial cells.

      Molecular Weight32 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in bEnd.3 cells.

      Flow Cytometry Analysis: A 1:1,000 dilution of this antibody detected JAM-A in bEnd.3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-JAM-A, clone BV11 - 2458035 2458035
      Anti-JAM-A, clone BV11 - 2329186 2329186
      Anti-JAM-A, clone BV11 - NRG1897810 NRG1897810
      Anti-JAM-A, clone BV11 -2618683 2618683
      Anti-JAM-A, clone BV11 -2636744 2636744
      Anti-JAM-A, clone BV11 -2659649 2659649
      Anti-JAM-A, clone BV11 -2744124 2744124
      Anti-JAM-A, clone BV11- 2828352 2828352

      References

      Reference overviewPub Med ID
      JAM-A mediates neutrophil transmigration in a stimulus-specific manner in vivo: evidence for sequential roles for JAM-A and PECAM-1 in neutrophil transmigration.
      Woodfin, A; Reichel, CA; Khandoga, A; Corada, M; Voisin, MB; Scheiermann, C; Haskard, DO; Dejana, E; Krombach, F; Nourshargh, S
      Blood 110 1848-56 2007

      Show Abstract
      17505016 17505016
      Expression and function of junctional adhesion molecule-C in myelinated peripheral nerves.
      Scheiermann, C; Meda, P; Aurrand-Lions, M; Madani, R; Yiangou, Y; Coffey, P; Salt, TE; Ducrest-Gay, D; Caille, D; Howell, O; Reynolds, R; Lobrinus, A; Adams, RH; Yu, AS; Anand, P; Imhof, BA; Nourshargh, S
      Science (New York, N.Y.) 318 1472-5 2007

      Show Abstract
      18048693 18048693
      Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration.
      Martìn-Padura, I; Lostaglio, S; Schneemann, M; Williams, L; Romano, M; Fruscella, P; Panzeri, C; Stoppacciaro, A; Ruco, L; Villa, A; Simmons, D; Dejana, E
      The Journal of cell biology 142 117-27 1998

      Show Abstract
      9660867 9660867