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MABS1281 | Anti-Itk Antibody, clone 2F12

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IP M Purified Monoclonal Antibody
      Catalogue NumberMABS1281
      DescriptionAnti-Itk Antibody, clone 2F12
      Alternate Names
      • Tyrosine-protein kinase ITK/TSK
      • IL-2-inducible T-cell kinase
      • Interleukin-2-inducible T-cell kinase
      • Kinase EMT
      • T-cell-specific kinase
      • Tyrosine-protein kinase Lyk
      Background InformationTyrosine-protein kinase ITK/TSK (EC; UniProt Q08881; also known as IL-2-inducible T-cell kinase, Interleukin-2-inducible T-cell kinase, Kinase EMT) (T-cell-specific kinase, Tyrosine-protein kinase Lyk) is encoded by the ITK (also known as EMT, LPFS1, LYK) gene (Gene ID 3702) in human. Interleukin-2-inducible T-cell kinase (Itk) is a TEC family non-receptor tyrosine kinase that plays an important roles in signal transduction in T-cells. Upon cell surface receptor stimulation, Itk is recruited to the membrane by the adaptor molecule LAT (linker for activated T cells), where Itk is transphosphorylated at Tyr511 by Lck, Itk Tyr180 then takes place for full activation. Once activated, Itk transduces cellular signaling by activating phospholipase Cγ1 (PLCγ1), leading to activation of nuclear factor of activated T-cells (NFAT), nuclear factor κB (NFκB), and mitogen-activated protein kinase (MAPK) pathways. Itk-/- mice are resistant to developing ovalbumin-induced asthma and exhibit reduced cytokines production by iNKT cells, as well as reduced Th2 effector cytokines and IL-17A productin by T-cells.
      Product Information
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect ITK using this mouse monoclonal Anti-Itk Antibody, clone 2F12, Cat. No. MABS1281, validated for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A 1:1000 dilution from a representative lot detected Itk in 10 µg of HuT-78 human T-cell lymphoma cell lysate.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Itk from both parental and Lck-negative (J.CaM1) Jurkat cells. Itk immunoprecipitated from the parental Jurkat cells was found with increased tyrosine phosphorylation following TCR cross-linking or pervanadate treatment, while only very weak tyrosine phosphorylation was seen with Itk from J.CaM1 upon prolonged pervanadate treatment (Heyeck, S.D., et al. (1997). J. Biol. Chem. 272(40):25401-25408).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated tyrosine-phosphorylated Itk from Jurkat cells stimulated by cross-linking of endogenous cell surface CD3 or transfected CD8 extracellular domain/CD28 cytoplasmic domain chimera (Heyeck, S.D., et al. (1997). J. Biol. Chem. 272(40):25401-25408).

      Western Blotting Analysis: A representative lot detected baculovirus expressed Itk from infected Sf9 insect cells following ltk immunoprecipitation by the same antibody. The overexpressed ltk was found autophosphorylated on tyrosine when expressed alone in Sf9 and increased ltk tyrosine phosphorylation was seen when co-expressed with Lck (Heyeck, S.D., et al. (1997). J. Biol. Chem. 272(40):25401-25408).
      Biological Information
      ImmunogenGST-tagged recombinant human/mouse/rat Itk N-terminal fragment.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 2F12 targets the N-terminal region of human, mouse, and rat Itk.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rat, Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITK
      • EMT
      • LPFS1
      • LYK
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~71 kDa observed. 71.83/72.29/72.49 (human/mouse/rat) kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Itk in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information