A part of MilliporeSigma

MABC171 | Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H Antibody, clone HMab-1

MABC171
200 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IHC, ICC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABC171
      DescriptionAnti-Isocitrate Dehydrogenase 1 (IDH1)-R132H Antibody, clone HMab-1
      Alternate Names
      • Isocitrate dehydrogenase [NADP] cytoplasmic
      • IDH
      • Cytosolic NADP-isocitrate dehydrogenase
      • IDP
      • NADP(+)-specific ICDH
      • Oxalosuccinate decarboxylase
      Background InformationIsocitrate dehydrogenase 1 (IDH1) is a 46 kDa NADP-dependent enzyme, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate. IDH1 may also play a role in the prevention of oxidative damage, and the shuttling of proteins to peroxisomes. It is widely reported that mutations in IDH1 result in various types of glioma.
      References
      Product Information
      FormatPurified
      Control
      • MBP tagged recombinant Isocitrate Dehydrogenase 1 (IDH1)
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect Isocitrate Dehydrogenase 1 (IDH1)-R132H using this Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H Antibody, clone HMab-1 validated for use in Western Blotting, IHC, ICC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: A representative lot of this antibody detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in a panel of wild-type and mutated recombinant IDH1 proteins.

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in anaplastic and diffuse astrocytoma tissues and in primary glioblastoma tissues (Kato, Y., et al. (2009). Biochem Biophys Res Commun. 390(3):547-51.).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in transfected glioblastoma and transfected LN229 cells (Kaneko, M. K., et al. (2011). Biochem Biophys Res Commun. 406(4):608-613.).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Isocitrate Dehydrogenase 1 (IDH1).
      CloneHMab-1
      HostMouse
      SpecificityThis antibody is specific for the R132H mutation of Isocitrate Dehydrogenase 1 (IDH1).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IDH1
      • PICD
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~90 kDa observed. The calculated molecular weight of Isocitrate Dehydrogenase 1 (IDH1) is 47 kDA. However, a Western Blot was performed using an MBP-tagged recombinant Isocitrate Dehydrogenase 1 (IDH1). Because MBP has a molecular weight of ~42 kDa, a band was observed at ~90 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in MBP-tagged recombinant Isocitrate Dehydrogenase 1 (IDH1).

      Western Blot Analysis: A 1:500 dilution of this antibody detected MBP tagged recombinant Isocitrate Dehydrogenase 1 (IDH1) in WB.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 - 2370195 2370195
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 - 2388344 2388344
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 - 2436804 2436804
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 - 2452474 2452474
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 - 2272163 2272163
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 - Q2058409 Q2058409
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 -2504321 2504321
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 -2552649 2552649
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 -2604845 2604845
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 -2687834 2687834
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 -2712566 2712566
      Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H, clone HMab-1 -2759819 2759819
      Anti-Isocitrate Dehydrogenase 1 -2789402 2789402
      Anti-Isocitrate Dehydrogenase 1 -2806529 2806529
      Anti-Isocitrate Dehydrogenase 1 -2822032 2822032

      References

      Reference overviewPub Med ID
      Intraoperative mass spectrometry mapping of an onco-metabolite to guide brain tumor surgery.
      Santagata, S; Eberlin, LS; Norton, I; Calligaris, D; Feldman, DR; Ide, JL; Liu, X; Wiley, JS; Vestal, ML; Ramkissoon, SH; Orringer, DA; Gill, KK; Dunn, IF; Dias-Santagata, D; Ligon, KL; Jolesz, FA; Golby, AJ; Cooks, RG; Agar, NY
      Proceedings of the National Academy of Sciences of the United States of America 111 11121-6 2014

      Show Abstract
      24982150 24982150
      Establishment of a novel monoclonal antibody SMab-1 specific for IDH1-R132S mutation.
      Kaneko, Mika Kato, et al.
      Biochem. Biophys. Res. Commun., 406: 608-13 (2011) 2011

      Show Abstract
      21352804 21352804
      A monoclonal antibody IMab-1 specifically recognizes IDH1R132H, the most common glioma-derived mutation.
      Kato, Yukinari, et al.
      Biochem. Biophys. Res. Commun., 390: 547-51 (2009) 2009

      Show Abstract
      19818334 19818334

      Related Products & Applications

      Related Products

      Catalog Number Description  
      ABC1483 Anti-IDH1 Antibody Show Pricing & Availability
      ABE454 Anti-IDH Antibody Show Pricing & Availability
      ABN267 Anti-IDH1 Antibody Show Pricing & Availability
      MABC141 Anti-IDH1 Antibody, clone 12D6.1 Show Pricing & Availability
      MABC170 Anti-Isocitrate dehydrogenase - IDH1 Antibody, clone r41/W09 Show Pricing & Availability
      MABC197 Anti-Isocitrate Dehydrogenase 1 - IDH1 Antibody, clone RMab-3 Show Pricing & Availability
      AP124P Goat Anti-Mouse IgG Antibody, Peroxidase Conjugated, H+L Show Pricing & Availability
      MABC187 Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132S Antibody, clone SMab-1 Show Pricing & Availability

      View Gene Related Products for this Target

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies