Key Specifications Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|Description||Anti-Interferon-α Antibody, clone ST47|
|Presentation||Liquid, no preservatives|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.|
|Material Size||500 µL|
|Reference overview||Pub Med ID|
|Monoclonal antibodies that distinguish between subspecies of human interferon-alpha and that detect interferon oligomers. |
Shearer, M, et al.
J. Immunol., 133: 3096-101 (1984) 1984
Monoclonal antibodies to human interferons (HuIFN) of the alpha-class have been prepared by screening against 125I-labeled IFN in a rapid liquid-phase radioimmunoassay. All of the six antibodies produced react with HuIFN-alpha 2 and with some components of HuIFN-alpha N (Namalwa); three of the antibodies also bind HuIFN-alpha 1, and these either do not bind or bind very weakly the 25K component of Namalwa. Reaction of the antibodies with IFN components blotted onto nitrocellulose after separation on reducing gels suggests that two of the antibodies are against conformational determinants, whereas the epitopes recognized by the other antibodies are not destroyed by reduction or SDS treatment; these antibodies can be used to detect the presence of oligomers in IFN preparations. From the reaction of the antibodies with different alpha-IFN in immunoblots, in an antiviral assay, and in an ELISA, it was concluded that at least five different epitopes are recognized by the six antibodies, only one of which is non-neutralizing.
|Anti-Interferon-alpha, clone ST47 - Data Sheet|