MAB1976Z Anti-Integrin αVβ3 Antibody, clone LM609, Azide Free

MAB1976Z
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, B, Ca, Ch, H, Mk, Po, Rb FC, IHC, IP, FUNC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1976Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin αVβ3 Antibody, clone LM609, Azide Free
      Alternate Names
      • VitronectinReceptor
      • CD51/CD61
      • MAB1976
      Background InformationThe integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control: Human myeloma cell lines M21 and MoαV (Chen, 1995).
        Negative Control: Mo cells (this cell line was derived from M21 but does not express integrin αV
      • Chen, 1995)
      PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
      Applications
      ApplicationThis Anti-Integrin αVβ3 Antibody, clone LM609, Azide Free is validated for use in FC, IH, IP, FUNC for the detection of Integrin αVβ3.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Affects Function
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesFlow Cytometry: 1-3 μg LM609 per 0.5 x 10e6 cells in 100μL (Smith, 1996).

      Immunoprecipitation: specifically immunoprecipitates the integrin alphaVbeta3 complex which, when resolved by SDS-PAGE under reducing conditions, results in an approximately 130 kDa band corresponding to the alphaV subunit and a 105 kDa band corresponding to the beta3 subunit (Cheresh, 1987).

      Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections.

      Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 μg/mL.

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenPurified adhesion receptor from the human melanoma cell line M21 (Cheresh, 1987).
      CloneLM609
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, 1987). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, 1994). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Bovine
      • Canine
      • Chicken
      • Human
      • Monkey
      • Pig
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
      Gene Symbol
      • ITGAV
      • DKFZp686A08142
      • MSK8
      • CD51
      • VNRA
      Non-Reactive Species
      • Mouse
      • Rat
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1048 amino acids; 116038 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Molecular WeightMolecular Weight of Integrin αV: 125-135 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2–8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN alphaValphaVbeta3 [CD51/61] -25857682585768
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta3 [CD51/61] MONOCLONAL ANTIBODY - 23911192391119
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta3 [CD51/61] - 25102822510282
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta3 [CD51/61] -25757442575744
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta3 [CD51/61] -26087662608766
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta3 [CD51/61] -26777822677782
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta3 [CD51/61] -27123962712396
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta3 [CD51/61] -27664862766486
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta3 [CD51/61] -27898042789804
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta3 [CD51/61] -28148582814858

      References

      Reference overviewApplicationSpeciesPub Med ID
      Combined targeting of TGF-β1 and integrin β3 impairs lymph node metastasis in a mouse model of non-small-cell lung cancer.
      Salvo, E; Garasa, S; Dotor, J; Morales, X; Peláez, R; Altevogt, P; Rouzaut, A
      Molecular cancer  13  112  2014

      Show Abstract
      24884715 24884715
      High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress.
      Shin, ES; Huang, Q; Gurel, Z; Sorenson, CM; Sheibani, N
      PloS one  9  e103148  2014

      Show Abstract
      25068294 25068294
      Roles of the putative integrin-binding motif of the human metapneumovirus fusion (f) protein in cell-cell fusion, viral infectivity, and pathogenesis.
      Wei, Y; Zhang, Y; Cai, H; Mirza, AM; Iorio, RM; Peeples, ME; Niewiesk, S; Li, J
      Journal of virology  88  4338-52  2014

      Show Abstract
      24478423 24478423
      Synstatin: a selective inhibitor of the syndecan-1-coupled IGF1R-αvβ3 integrin complex in tumorigenesis and angiogenesis.
      Rapraeger, Alan C
      FEBS J., (2013)  2013

      Show Abstract
      23375101 23375101
      Modulation of vascular cell function by bim expression.
      Morrison, ME; Palenski, TL; Jamali, N; Sheibani, N; Sorenson, CM
      International journal of cell biology  2013  297537  2013

      Show Abstract
      24288535 24288535
      Dual inhibition of αV integrins and Src kinase activity as a combination therapy strategy for colorectal cancer.
      Jia, J; Starodub, A; Cushman, I; Liu, Y; Marshall, DJ; Hurwitz, HI; Nixon, AB
      Anti-cancer drugs  24  237-50  2013

      Show Abstract
      23275294 23275294
      New blocking antibodies impede adhesion, migration and survival of ovarian cancer cells, highlighting MFGE8 as a potential therapeutic target of human ovarian carcinoma.
      Tibaldi, L; Leyman, S; Nicolas, A; Notebaert, S; Dewulf, M; Ngo, TH; Zuany-Amorim, C; Amzallag, N; Bernard-Pierrot, I; Sastre-Garau, X; Théry, C
      PloS one  8  e72708  2013

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)23977342 23977342
      BIM deficiency differentially impacts the function of kidney endothelial and epithelial cells through modulation of their local microenvironment.
      Sheibani, N; Morrison, ME; Gurel, Z; Park, S; Sorenson, CM
      American journal of physiology. Renal physiology  302  F809-19  2012

      Show Abstract
      ImmunofluorescenceMouse22169007 22169007
      The metastasis gene NEDD9 product acts through integrin β3 and Src to promote mesenchymal motility and inhibit amoeboid motility.
      Ahn, J; Sanz-Moreno, V; Marshall, CJ
      Journal of cell science  125  1814-26  2012

      Show Abstract
      22328516 22328516
      Exosome release of ADAM15 and the functional implications of human macrophage-derived ADAM15 exosomes.
      Lee, HD; Koo, BH; Kim, YH; Jeon, OH; Kim, DS
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  26  3084-95  2012

      Show Abstract
      22505472 22505472

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression
      An Introduction to Antibodies and Their Applications

      Data Sheet

      Title
      Anti-Integrin AlphaVBeta3, Clone LM609, Azide Free - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies