MAB1976 Anti-Integrin αVβ3 Antibody, clone LM609

MAB1976
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, B, Ca, Ch, H, Mk, Po, Rb FC, IF, IHC, IP, FUNC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1976
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin αVβ3 Antibody, clone LM609
      Alternate Names
      • Vitronectin Receptor
      • CD51/CD61
      Background InformationThe integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors..
      References
      Product Information
      FormatPurified
      Control
      • Positive Control: Human myeloma cell lines M21 and MoαV (Chen, 1995).
        Negative Control: Mo cells (this cell line was derived from M21 but does not express integrin αV
      • Chen, 1995)
      PresentationImmunoglobulin was Purified by protein A affinity and is presented as a liquid in 0.02M PB, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
      Applications
      ApplicationAnti-Integrin αVβ3 Antibody, clone LM609 is an antibody against Integrin αVβ3 for use in FC, IF, IH and IP and has published in over 60 citations.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Affects Function
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesFlow Cytometry: 1-3 μg LM609 per 0.5 x 10e6 cells in 100μL (Smith, 1996).

      Immunoprecipitation: specifically immunoprecipitates the integrin alphaVbeta3 complex which, when resolved by SDS-PAGE under reducing conditions, results in an approximately 130 kDa band corresponding to the alphaV subunit and a 105 kDa band corresponding to the beta3 subunit (Cheresh, 1987).

      Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections.

      Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 μg/mL.

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenPurified adhesion receptor from the human melanoma cell line M21 (Cheresh, 1987).
      CloneLM609
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, 1987). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, 1994). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Bovine
      • Canine
      • Chicken
      • Human
      • Monkey
      • Pig
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
      Gene Symbol
      • ITGAV
      • DKFZp686A08142
      • MSK8
      • CD51
      • VNRA
      Non-Reactive Species
      • Mouse
      • Rat
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1048 amino acids; 116038 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Molecular Weight150 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN #945;V#946;3 (VITRONECTIN RECEPTOR) MONOCLONAL ANTIBODY -
      MOUSE ANTI-HUMAN INTEGRIN aVB3 (VITRONECTIN RECEPTOR) MONOCLONAL ANTIBODY2922395
      MOUSE ANTI-HUMAN INTEGRIN aVb3 (VITRONECTIN RECEPTOR) - 22110302211030
      MOUSE ANTI-HUMAN INTEGRIN aVb3 (VITRONECTIN RECEPTOR) MONOCLONAL ANTIBODY - 19846721984672
      MOUSE ANTI-HUMAN INTEGRIN aVb3 (VITRONECTIN RECEPTOR) MONOCLONAL ANTIBODY - 20199732019973
      MOUSE ANTI-HUMAN INTEGRIN aVb3 (VITRONECTIN RECEPTOR) MONOCLONAL ANTIBODY - 20427842042784
      MOUSE ANTI-HUMAN INTEGRIN aVb3 (VITRONECTIN RECEPTOR) MONOCLONAL ANTIBODY - 23257622325762
      MOUSE ANTI-HUMAN INTEGRIN αVβ32474925
      MOUSE ANTI-HUMAN INTEGRIN αVβ3 (VITRONECTIN RECEPTOR) MONOCLONAL ANTIBODY - 24532482453248
      MOUSE ANTI-HUMAN INTEGRIN αVβ3 (VITRONECTIN RECEPTOR) - 29841272984127

      References

      Reference overviewApplicationSpeciesPub Med ID
      Endothelial destabilization by angiopoietin-2 via integrin β1 activation.
      Hakanpaa, L; Sipila, T; Leppanen, VM; Gautam, P; Nurmi, H; Jacquemet, G; Eklund, L; Ivaska, J; Alitalo, K; Saharinen, P
      Nature communications  6  5962  2015

      Show Abstract
      Immunofluorescence25635707 25635707
      H-CRRETAWAC-OH, a lead structure for the development of radiotracer targeting integrin α5β1?
      Haubner, R; Maschauer, S; Einsiedel, J; Eder, IE; Rangger, C; Gmeiner, P; Virgolini, IJ; Prante, O
      BioMed research international  2014  243185  2014

      Show Abstract
      25374888 25374888
      Identification of platelet function defects by multi-parameter assessment of thrombus formation.
      de Witt, SM; Swieringa, F; Cavill, R; Lamers, MM; van Kruchten, R; Mastenbroek, T; Baaten, C; Coort, S; Pugh, N; Schulz, A; Scharrer, I; Jurk, K; Zieger, B; Clemetson, KJ; Farndale, RW; Heemskerk, JW; Cosemans, JM
      Nature communications  5  4257  2014

      Show Abstract
      25027852 25027852
      Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation.
      Klingberg, F; Chow, ML; Koehler, A; Boo, S; Buscemi, L; Quinn, TM; Costell, M; Alman, BA; Genot, E; Hinz, B
      The Journal of cell biology  207  283-97  2014

      Show Abstract
      25332161 25332161
      Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress.
      Zhao, Y; Wang, S; Sorenson, CM; Teixeira, L; Dubielzig, RR; Peters, DM; Conway, SJ; Jefcoate, CR; Sheibani, N
      Molecular and cellular biology  33  4225-40  2013

      Show Abstract
      Dot BlotMouse23979599 23979599
      Integrin control of the transforming growth factor-β pathway in glioblastoma.
      Roth, P; Silginer, M; Goodman, SL; Hasenbach, K; Thies, S; Maurer, G; Schraml, P; Tabatabai, G; Moch, H; Tritschler, I; Weller, M
      Brain : a journal of neurology  136  564-76  2013

      Show Abstract
      Immunohistochemistry23378223 23378223
      The differentiation of pancreatic tumor-initiating cells by vitronectin can be blocked by cilengitide.
      Cabarcas, SM; Sun, L; Mathews, L; Thomas, S; Zhang, X; Farrar, WL
      Pancreas  42  861-70  2013

      Show Abstract
      23462327 23462327
      Apolipoprotein(a) acts as a chemorepellent to human vascular smooth muscle cells via integrin αVβ3 and RhoA/ROCK-mediated mechanisms.
      Riches, K; Franklin, L; Maqbool, A; Peckham, M; Adams, M; Bond, J; Warburton, P; Feric, NT; Koschinsky, ML; O'Regan, DJ; Ball, SG; Turner, NA; Porter, KE
      The international journal of biochemistry & cell biology  45  1776-83  2013

      Show Abstract
      23726972 23726972
      Efficient downregulation of VEGF in retinal pigment epithelial cells by integrin ligand-labeled liposome-mediated siRNA delivery.
      Chen, CW; Yeh, MK; Shiau, CY; Chiang, CH; Lu, DW
      International journal of nanomedicine  8  2613-27  2013

      Show Abstract
      23901275 23901275
      The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma–associated herpesvirus.
      Hahn, AS; Kaufmann, JK; Wies, E; Naschberger, E; Panteleev-Ivlev, J; Schmidt, K; Holzer, A; Schmidt, M; Chen, J; König, S; Ensser, A; Myoung, J; Brockmeyer, NH; Stürzl, M; Fleckenstein, B; Neipel, F
      Nature medicine  18  961-6  2012

      Show Abstract
      22635007 22635007

      Data Sheet

      Title
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta3 (VITRONECTIN RECEPTOR)

      FAQ

      QuestionAnswer
      Does LM609 work in western blots?No, the epitope recognized by LM609 is to the complex of Av and B3 together, thus when run on gels and denatured that epitope is physically destroyed, thus non-reactive.
      Does this product react with paraffin embedded tissues?Some researchers have gotten LM609 to react with traditionally fixed paraffin tissue, however Millipore cannot endorse the use of paraffin tissues with LM609 at this time.
      Does LM609 react with rat or mouse?LM609 traditionally has not worked with rat or mouse tissues, however one can find reports of LM609 working with rat under some special conditions. At this time Millipore does not endorse the use of LM609 with mouse or rat tissues, however.
      What fixation works best for LM609?Because the epitope of LM609 is fragile acetone is the preferred fixation, however short fixations in paraformaldehyde usually presents no serious difficulties.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies