AB1920 Anti-Integrin α3 Antibody

AB1920
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ca, Ch, Gt, Ht, Eq, H, M, Po, R, Sh ELISA, IF, IHC, IP, RIA, WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1920
      ReplacesAB1948
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin α3 Antibody
      Alternate Names
      • CD49c
      Background InformationIntegrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 19 alpha and 8 beta subunits that form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. Both of the subunits contribute to the binding of the ligand. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.
      References
      Product Information
      FormatSerum
      Control
      • Human fetal foreskin fibroblasts (HFFF), neuroblastomas
      PresentationRabbit polyclonal IgG antiserum containing 0.05% sodium azide.
      Applications
      ApplicationDetect Integrin α3 using this Anti-Integrin α3 Antibody validated for use in ELISA, IF, IH, IP, RIA & WB.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Radioimmunoassay
      • Western Blotting
      Application NotesELISA/RIA:
      A 1:1,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay/Radioimmunoassay.

      Immunoprecipitation:
      5 µL of a previous lot precipitated alpha3/beta1 from 5 x 106 cells (Sherry, D.M., 2001).

      Immunohistochemistry/immunofluorescence:
      1:500 dilution of a previous lot was used, suggest use on acetone-fixed tissue.

      Western Blot Analysis:
      1:500 dilution of a previous lot was used.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA synthetic peptide (CRIQPSETERLTDDY) derived from the COOH terminal sequence located in the cytoplasmic domain of the human alpha3 integrin subunit.
      EpitopeCytoplasmic domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityIntegrin alpha3. Specificity determined by immunoprecipitation from a detergent extract of 35S-methionine labeled neuroblastoma cells. No cross-reactivity to alpha1, alpha2, alpha4, alpha5, alpha6, or alphaV integrins.
      Species Reactivity
      • Bovine
      • Canine
      • Chicken
      • Goat
      • Hamster
      • Horse
      • Human
      • Mouse
      • Pig
      • Rat
      • Sheep
      Species Reactivity NoteHuman, mouse, rat, hamster, bovine, equine, sheep, porcine, canine, chick, and goat. Reactivity with other species has not been determined.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryITGA3 encodes the integrin alpha 3 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 3 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that interacts with many extracellular-matrix proteins. The alpha 3 beta 1 integrin is known variously as: very late (activation) antigen 3 ('VLA-3'), very common antigen 2 ('VCA-2'), extracellular matrix receptor 1 ('ECMR1'), and galactoprotein b3 ('GAPB3').
      Gene Symbol
      • ITGA3
      • GAP-B3
      • CD49c
      • FLJ34704
      • MSK18
      • VLA3a
      • VL3A
      • VCA-2
      • FLJ34631
      • FRP-2
      • GAPB3
      • CD49C
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P26006 # Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha- 3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
      SIZE: 1066 amino acids; 118698 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-3 associates with beta-1. Interacts with HPS5. />SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
      PTM: Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12- myristate 13-acetate stimulation.
      SIMILARITY: SwissProt: P26006 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Molecular Weight130 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 lysates.

      Western blot: 1:500 dilution of this lot detected INTEGRIN ALPHA 3 on 10 μg of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Integrin #945;3 - 20184552018455
      Anti-Integrin 3 - 19792541979254
      Anti-Integrin 3 - 20614272061427
      Anti-Integrin 3 - 21971712197171
      Anti-Integrin 3 - JBC1774746JBC1774746
      Anti-Integrin 3 - JC1604247JC1604247
      Anti-Integrin 3 - JC1673164JC1673164
      Anti-Integrin 3 - LV1580994LV1580994
      Anti-Integrin 3 - NG1713410NG1713410
      Anti-Integrin 3 - NG1740913NG1740913

      References

      Reference overviewApplicationSpeciesPub Med ID
      Mitochondrial uncoupling links lipid catabolism to Akt inhibition and resistance to tumorigenesis.
      Nowinski, SM; Solmonson, A; Rundhaug, JE; Rho, O; Cho, J; Lago, CU; Riley, CL; Lee, S; Kohno, S; Dao, CK; Nikawa, T; Bratton, SB; Wright, CW; Fischer, SM; DiGiovanni, J; Mills, EM
      Nature communications  6  8137  2015

      Show Abstract
      Western Blotting26310111 26310111
      Integrin α3β1 regulates kidney collecting duct development via TRAF6-dependent K63-linked polyubiquitination of Akt.
      Yazlovitskaya, EM; Tseng, HY; Viquez, O; Tu, T; Mernaugh, G; McKee, KK; Riggins, K; Quaranta, V; Pathak, A; Carter, BD; Yurchenco, P; Sonnenberg, A; Böttcher, RT; Pozzi, A; Zent, R
      Molecular biology of the cell  26  1857-74  2015

      Show Abstract
      25808491 25808491
      MicroRNA-4723 inhibits prostate cancer growth through inactivation of the Abelson family of nonreceptor protein tyrosine kinases.
      Arora, S; Saini, S; Fukuhara, S; Majid, S; Shahryari, V; Yamamura, S; Chiyomaru, T; Deng, G; Tanaka, Y; Dahiya, R
      PloS one  8  e78023  2013

      Show Abstract
      Western Blotting24223753 24223753
      SEL1L regulates adhesion, proliferation and secretion of insulin by affecting integrin signaling.
      Diaferia, GR; Cirulli, V; Biunno, I
      PloS one  8  e79458  2013

      Show Abstract
      24324549 24324549
      Human umbilical cord mesenchymal stromal cells exhibit immature nucleus pulposus cell phenotype in a laminin-rich pseudo-three-dimensional culture system.
      Chon, BH; Lee, EJ; Jing, L; Setton, LA; Chen, J
      Stem cell research & therapy  4  120  2013

      Show Abstract
      24405888 24405888
      Integrin upregulation and localization to focal adhesion sites in pregnant human myometrium.
      Burkin, HR; Rice, M; Sarathy, A; Thompson, S; Singer, CA; Buxton, IL
      Reproductive sciences (Thousand Oaks, Calif.)  20  804-12  2013

      Show Abstract
      23298868 23298868
      Adhesion proteins--an impact on skeletal myoblast differentiation.
      Przewoźniak, M; Czaplicka, I; Czerwińska, AM; Markowska-Zagrajek, A; Moraczewski, J; Stremińska, W; Jańczyk-Ilach, K; Ciemerych, MA; Brzoska, E
      PloS one  8  e61760  2013

      Show Abstract
      Immunofluorescence23671573 23671573
      Viral infection of the pregnant cervix predisposes to ascending bacterial infection.
      Racicot, K; Cardenas, I; Wünsche, V; Aldo, P; Guller, S; Means, RE; Romero, R; Mor, G
      Journal of immunology (Baltimore, Md. : 1950)  191  934-41  2013

      Show Abstract
      Western Blotting23752614 23752614
      Podocyte-specific knockout of myosin 1e disrupts glomerular filtration.
      Chase, SE; Encina, CV; Stolzenburg, LR; Tatum, AH; Holzman, LB; Krendel, M
      American journal of physiology. Renal physiology  303  F1099-106  2012

      Show Abstract
      22811491 22811491
      Alteration of the actin cytoskeleton and localisation of the α6β1 and α3 integrins during regeneration of the rat submandibular gland.
      Osamu Shimizu,Hiroshi Shiratsuchi,Koichiro Ueda,Shunichi Oka,Yoshiyuki Yonehara
      Archives of oral biology  57  2012

      Show Abstract
      22410146 22410146

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies