MAB2247 Anti-Integrin β1 Antibody, clone 12G10

MAB2247
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ELISA, FC, IP, FUNC, CULT, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB2247
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β1 Antibody, clone 12G10
      Alternate Names
      • Integrin beta-1
      • CD29
      • Glycoprotein Iia
      • GPIIA
      • Integrin VLA-4 subunit beta
      • Very late activation protein
      • beta polypeptide
      Background InformationIntegrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
      References
      Product Information
      FormatPurified
      Control
      • Tonsil, human skin tissue
      PresentationPurified immunoglobulin. Liquid in phosphate buffer saline, with 0.1% sodium azide.
      Applications
      ApplicationAnti-Integrin β1 Antibody, clone 12G10 detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IP, FUNC, CULT & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Affects Function
      • Cell Culture
      • Western Blotting
      Application NotesFlow cytometry: 1:25-1:50

      ELISA : 10 μg/mL

      Western Blot: known to react with human beta 1 integrin subunit under non-reduced conditions only {Mould, 1995}.

      Stimulatory to alpha5beta1 interaction with fibronectin {Mould, 1995}.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.
      Clone12G10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB2247 recognizes the human beta1 integrin. This antibody has been shown to recognize a ligand-induced binding site, with antibody binding increasing in the presence of a fibronectin ligand. This antibody also enhances alpha 5 - beta 1 fibronectin interactions. MAB2247 does not cross-react with mouse or rat samples.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITGB1
      • FNRB
      • MDF2
      • MSK12
      • VLA-BETA
      • VLAB
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight88 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2–8°C in undiluted aliquots for up one month. For long term storage maintain at -20°C for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Integrin β1 Antibody, clone 12G10 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Integrin β1 Antibody, clone 12G10 Certificates of Analysis

      TitleLot Number
      MAB2247 - 20808352080835
      MOUSE ANTI HUMAN CD 29 [INTEGRIN #946;1] - 21518362151836
      MOUSE ANTI HUMAN CD 29 [INTEGRIN B1] MONOCLONAL ANTIBODY2916192
      MOUSE ANTI HUMAN CD 29 [INTEGRIN B1] MONOCLONAL ANTIBODY2957383
      MOUSE ANTI HUMAN CD 29 [INTEGRIN b1] MONOCLONAL ANTIBODY - 23021532302153
      MOUSE ANTI HUMAN CD 29 [INTEGRIN β1]2842102
      MOUSE ANTI HUMAN CD 29 [INTEGRIN β1] MONOCLONAL ANTIBODY - 23874742387474
      MOUSE ANTI HUMAN CD 29 [INTEGRIN β1] - 31886863188686
      MOUSE ANTI HUMAN CD 29 [INTEGRIN β1] - 32451723245172
      MOUSE ANTI HUMAN CD 29 [INTEGRIN β1] - 32475253247525

      References

      Reference overviewPub Med ID
      Lumican expression, localization and antitumor activity in prostate cancer.
      Coulson-Thomas, VJ; Coulson-Thomas, YM; Gesteira, TF; Andrade de Paula, CA; Carneiro, CR; Ortiz, V; Toma, L; Kao, WW; Nader, HB
      Experimental cell research  319  967-81  2013

      Show Abstract
      23399832 23399832
      High expression of N-acetylglucosaminyltransferase IVa promotes invasion of choriocarcinoma.
      Niimi, K; Yamamoto, E; Fujiwara, S; Shinjo, K; Kotani, T; Umezu, T; Kajiyama, H; Shibata, K; Ino, K; Kikkawa, F
      British journal of cancer  107  1969-77  2012

      Show Abstract
      23169300 23169300
      MMP7 shedding of syndecan-1 facilitates re-epithelialization by affecting alpha(2)beta(1) integrin activation.
      Chen, Peter, et al.
      PLoS ONE, 4: e6565 (2009)  2009

      Show Abstract
      19668337 19668337
      N-acetylglucosaminyltransferase V regulates extravillous trophoblast invasion through glycosylation of alpha5beta1 integrin.
      Yamamoto, E; Ino, K; Miyoshi, E; Inamori, K; Abe, A; Sumigama, S; Iwase, A; Kajiyama, H; Shibata, K; Nawa, A; Kikkawa, F
      Endocrinology  150  990-9  2009

      Show Abstract
      18845630 18845630
      Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis.
      Yamamoto, E; Ino, K; Miyoshi, E; Shibata, K; Takahashi, N; Kajiyama, H; Nawa, A; Nomura, S; Nagasaka, T; Kikkawa, F
      British journal of cancer  97  1538-44  2007

      Show Abstract Full Text Article
      17971775 17971775
      Caveolin-1 mediates the expression and localization of cathepsin B, pro-urokinase plasminogen activator and their cell-surface receptors in human colorectal carcinoma cells.
      Cavallo-Medved, D; Mai, J; Dosescu, J; Sameni, M; Sloane, BF
      Journal of cell science  118  1493-503  2005

      Show Abstract
      15769846 15769846
      Src-induced de-regulation of E-cadherin in colon cancer cells requires integrin signalling.
      Egle Avizienyte, Anne W Wyke, Robert J Jones, Gordon W McLean, M Andrew Westhoff, Valerie G Brunton, Margaret C Frame
      Nature cell biology  4  632-8  2002

      Show Abstract
      12134161 12134161
      The cysteine-rich domain of human ADAM 12 supports cell adhesion through syndecans and triggers signaling events that lead to beta1 integrin-dependent cell spreading.
      Iba, K, et al.
      J. Cell Biol., 149: 1143-56 (2000)  2000

      Show Abstract
      10831617 10831617
      Regulation of integrin function: evidence that bivalent-cation-induced conformational changes lead to the unmasking of ligand-binding sites within integrin alpha5 beta1.
      Mould, A P, et al.
      Biochem. J., 331 ( Pt 3): 821-8 (1998)  1998

      Show Abstract
      9560310 9560310
      Identification of a novel anti-integrin monoclonal antibody that recognises a ligand-induced binding site epitope on the beta 1 subunit.
      Mould, A P, et al.
      FEBS Lett., 363: 118-22 (1995)  1995

      Show Abstract
      7537221 7537221

      Data Sheet

      Title
      MOUSE ANTI HUMAN CD 29 [INTEGRIN β1]