A part of MilliporeSigma

MAB2060 | Anti-Integrin β4 Antibody, clone ASC-9

MAB2060
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, IP, IHC, FUNC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB2060
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β4 Antibody, clone ASC-9
      Alternate Names
      • CD104
      References
      Product Information
      FormatPurified
      PresentationLiquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Integrin β4 Antibody, clone ASC-9 is validated for use in FC, IP, IH, FUNC for the detection of Integrin β4.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Immunohistochemistry
      • Affects Function
      Application NotesInhibition of cell attachment: inhibits SCC9 cell adhesion to human and

      EHS laminin, but not to laminin GD-2 peptide, type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.

      Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.

      Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

      Immunoprecipitation: precipitates PAGE bands of 66, 76, 120, 135 and 200kDa (reduced) or 66, 110, 125, 130 and 190kDa (non-reduced) from surface-biotinylated SCC9 cells.

      Working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman squamous cell carcinoma cells (SCC9)
      CloneASC-9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityHuman beta4 integrin (CD104)
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • ITGB4
      • CD104
      • GP150
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16144 # Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
      SIZE: 1822 amino acids; 202151 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
      DOMAIN: SwissProt: P16144 The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
      DISEASE: SwissProt: P16144 # Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. & Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. This nonlethal form of junctional epidermolysis bullosa is characterized by life- long blistering of the skin, associated with hair and tooth abnormalities.
      SIMILARITY: Belongs to the integrin beta chain family. & Contains 1 Calx-beta domain. & Contains 4 fibronectin type-III domains. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN b4 [CD104] MONOCLONAL ANTIBODY - 2188478 2188478
      MOUSE ANTI-HUMAN INTEGRIN b4 [CD104] MONOCLONAL ANTIBODY - 2193300 2193300
      MOUSE ANTI-HUMAN INTEGRIN b4 [CD104] MONOCLONAL ANTIBODY - 2299961 2299961
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] -2593140 2593140
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] -2669944 2669944

      References

      Reference overviewApplicationSpeciesPub Med ID
      Role of integrins in the assembly and function of hensin in intercalated cells.
      Vijayakumar, S; Erdjument-Bromage, H; Tempst, P; Al-Awqati, Q
      Journal of the American Society of Nephrology : JASN 19 1079-91 2008

      Show Abstract Full Text Article
      Western Blotting, ImmunoprecipitationRabbit18337486 18337486
      A new DPB1 allele (DPB1*5901) identified by reverse dot blot hybridization and confirmed by DNA sequencing.
      Perrier, P and Dormoy, A
      Tissue Antigens, 47: 356-9 (1996) 1996

      8773330 8773330
      A novel recognition site on laminin for the alpha 3 beta 1 integrin.
      Pattaramalai, S, et al.
      Exp. Cell Res., 222: 281-90 (1996) 1996

      8598215 8598215

      Data Sheet

      Title
      Anti-Integrin beta4, clone ASC-9 - Data Sheet

      Related Products & Applications

      Related Products

      Catalog Number Description  
      AB1922 Anti-Integrin β4 Antibody Show Pricing & Availability
      FCMAB343F Milli-Mark™ Anti-Integrin β4-FITC Antibody, clone ASC-3 Show Pricing & Availability
      MAB1964 Anti-Integrin β4 Antibody, clone 3E1 Show Pricing & Availability
      MAB2058 Anti-Integrin β4 Antibody, clone ASC-3 Show Pricing & Availability
      MAB2058Z Anti-Integrin β4 Antibody, clone ASC-3, azide free Show Pricing & Availability
      MAB2059 Anti-Integrin β4 Antibody, clone ASC-8 Show Pricing & Availability
      MAB2059-C Anti-Integrin B4 Antibody, clone ASC-8, Azide & Ascites Free Show Pricing & Availability
      MAB2059Z Anti-Integrin β4 Antibody, clone ASC-8 (Azide Free) Show Pricing & Availability

      View Gene Related Products for this Target

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies