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MAB2059 | Anti-Integrin β4 Antibody, clone ASC-8

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, IP, IHC, FUNC M Purified Monoclonal Antibody
      Catalogue NumberMAB2059
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β4 Antibody, clone ASC-8
      Alternate Names
      • CD104
      • MAB2059Z
      Product Information
      PresentationLiquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
      ApplicationAnti-Integrin β4 Antibody, clone ASC-8 is an antibody against Integrin β4 for use in FC, IP, IH.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Immunohistochemistry
      • Affects Function
      Application NotesImmunoprecipitation: A representative lot of this antibody clone precipitated PAGE bands of 66, 76, 120, 135 and 200 kDa (reduced) or 66, 110, 125, 130 and 190 kDa (non-reduced) from surface-biotinylated SCC9 cells.

      Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue).

      Flow Cytometry: A representative lot of this antibody clone stained human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

      Functional Activity Assay: A representative lot of this antibody clone induced a sub-basal split at the basement membrane in non-wounded tissue. In addition, wound closure was significantly inhibited by ASC-8, as the epithelial tongue only covered 40 percent of the wound area at 120 hours post-wounding. These results demonstrate that the β4 adhesion-blocking antibody may have adverse effects on normal tissue (Egles, C., et al., 2010).

      Working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman squamous cell carcinoma cells (SCC9)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityHuman beta4 integrin (CD104)
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • ITGB4
      • CD104
      • GP150
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16144 # Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
      SIZE: 1822 amino acids; 202151 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
      DOMAIN: SwissProt: P16144 The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
      DISEASE: SwissProt: P16144 # Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. & Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. This nonlethal form of junctional epidermolysis bullosa is characterized by life- long blistering of the skin, associated with hair and tooth abnormalities.
      SIMILARITY: Belongs to the integrin beta chain family. & Contains 1 Calx-beta domain. & Contains 4 fibronectin type-III domains. & Contains 1 VWFA domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceFlow Cytometry Analysis:
      0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 A431 cells.
      0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN #946;4 [CD104] MONOCLONAL ANTIBODY - 2118020 2118020
      MOUSE ANTI-HUMAN INTEGRIN #946;4 [CD104] MONOCLONAL ANTIBODY - 2153019 2153019
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] -2579905 2579905
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] -2581310 2581310


      Reference overviewApplicationPub Med ID
      Combinatorial fibronectin and laminin signaling promote highly efficient cardiac differentiation of human embryonic stem cells.
      Sa, S; Wong, L; McCloskey, KE
      BioResearch open access 3 150-61 2014

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)25126479 25126479
      IκB kinase complex (IKK) triggers detachment-induced autophagy in mammary epithelial cells independently of the PI3K-AKT-MTORC1 pathway.
      Chen, N; Debnath, J
      Autophagy 9 1214-27 2013

      Show Abstract
      23778976 23778976
      Integrins as antimetastatic targets of RGD-independent snake venom components in liver metastasis [corrected].
      Rosenow, F; Ossig, R; Thormeyer, D; Gasmann, P; Schlüter, K; Brunner, G; Haier, J; Eble, JA
      Neoplasia (New York, N.Y.) 10 168-76 2008

      Show Abstract Full Text Article
      18283339 18283339
      Integrin regulation by vascular endothelial growth factor in human brain microvascular endothelial cells: role of alpha6beta1 integrin in angiogenesis.
      Lee, TH; Seng, S; Li, H; Kennel, SJ; Avraham, HK; Avraham, S
      The Journal of biological chemistry 281 40450-60 2006

      Show Abstract
      17085437 17085437
      A new DPB1 allele (DPB1*5901) identified by reverse dot blot hybridization and confirmed by DNA sequencing.
      Perrier, P and Dormoy, A
      Tissue Antigens, 47: 356-9 (1996) 1996

      8773330 8773330
      A novel recognition site on laminin for the alpha 3 beta 1 integrin.
      Pattaramalai, S, et al.
      Exp. Cell Res., 222: 281-90 (1996) 1996

      8598215 8598215

      Data Sheet

      Anti-Integrin beta4, clone ASC-8 - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies