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MAB1974 | Anti-Integrin β3 Antibody, clone BB10

50 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC, IHC M Purified Monoclonal Antibody
      Catalogue NumberMAB1974
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β3 Antibody, clone BB10
      Alternate Names
      • CD61
      Product Information
      PresentationPurified immunoglobulin. In PBS with 1% BSA and 0.1% sodium azide.
      ApplicationAnti-Integrin β3 Antibody, clone BB10 is an antibody against Integrin β3 for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoblotting: 1:1000 (Ylanne et al., 1988, 1990). The antibody is most effective when used against non-reduced samples.

      immunohistochemistry (indirect immunofluorescence)1:200 - 1:400. (Ylanne et al., 1988).

      Does not work on paraffin embedded sections.

      Final working dilutions must be determined by end user.
      Biological Information
      ImmunogenPartially purified platelet glycoprotein IIb/lIIa complex.
      SpecificityReacts with the beta-3 subunit of the integrin family, including GPIIIa of platelets. Recognized an extracellular epitope of beta-3.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
      Gene Symbol
      • ITGB3
      • GP3A
      • CD61
      • GPIIIa
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05106 # Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 788 amino acids; 87058 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
      PTM: Phosphorylated on tyrosine residues in response to thrombin- induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. SHC binding also occurs when Tyr-785 alone is phosphorylated.
      DISEASE: SwissProt: P05106 # Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.
      SIMILARITY: SwissProt: P05106 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain between 2 and 8°C up to 12 months.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN β3 -2726143 2726143


      Reference overviewPub Med ID
      Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation.
      Klingberg, F; Chow, ML; Koehler, A; Boo, S; Buscemi, L; Quinn, TM; Costell, M; Alman, BA; Genot, E; Hinz, B
      The Journal of cell biology 207 283-97 2014

      Show Abstract
      25332161 25332161
      External validation suggests Integrin beta 3 as prognostic biomarker in serous ovarian adenocarcinomas.
      Partheen, K; Levan, K; Osterberg, L; Claesson, I; Sundfeldt, K; Horvath, G
      BMC cancer 9 336 2009

      Show Abstract
      19775429 19775429
      Functional characterization of integrin alpha6beta4 adhesion interactions using soluble integrin constructs reveals the involvement of different functional domains in the beta4 subunit.
      Ling Ling Chen, Veronica Gabarra, Samuel Cho, Beth Browning, Xianjun Cao, Heather Huet, Anne Cheung, Ron Morena, Miriam Ramirez, Michael Shields, R Blake Pepinsky, Karen McLachlan, Ling Ling Chen, Veronica Gabarra, Samuel Cho, Beth Browning, Xianjun Cao, Heather Huet, Anne Cheung, Ron Morena, Miriam Ramirez, Michael Shields, R Blake Pepinsky, Karen McLachlan
      Cell communication adhesion 15 317-31 2008

      Show Abstract
      18979297 18979297
      Four potential biomarkers as prognostic factors in stage III serous ovarian adenocarcinomas.
      Karolina Partheen,Kristina Levan,Lovisa Osterberg,Ingela Claesson,Ghita Fallenius,Karin Sundfeldt,György Horvath
      International journal of cancer. Journal international du cancer 123 2008

      Show Abstract
      18709641 18709641
      Localization of beta 1, beta 3, alpha 5, alpha v, and alpha IIb subunits of the integrin family in spreading human erythroleukemia cells.
      Ylänne, J, et al.
      Blood, 76: 570-7 (1990) 1990

      Show Abstract
      1696147 1696147

      Data Sheet

      Anti-Integrin beta3, clone BB10 - Data Sheet

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