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MAB1962 | Anti-Integrin β2 Antibody, clone P4H9

MAB1962
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IHC, FUNC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1962
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β2 Antibody, clone P4H9
      Alternate Names
      • CD18
      References
      Product Information
      FormatPurified
      PresentationNon-sterile buffer of 0.02M phosphate, 0.25M Sodium Chloride, pH 7.6,

      0.1% sodium azide.
      Applications
      ApplicationDetect Integrin β2 using this Anti-Integrin β2 Antibody, clone P4H9 validated for use in IH.
      Key Applications
      • Immunohistochemistry
      • Affects Function
      Application NotesSuitable for use in immunofluorescence at 1:1000.

      Also suitable for aggregation inhibition assays using hematopoeitic cells or EBV-transformed B lymphocytes.

      For extensive dilution, protein-containing or other stabilizing medium should be used. Final working dilutions must be determined by end user.
      Biological Information
      CloneP4H9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      Specificitybeta2 integrin on hematopoeitic cells. Not present on platelets, endothelial cells, epithelial cells or fibroblasts.
      IsotypeIgG3
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe ITGB2 protein product is the integrin beta chain beta 2. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
      Gene Symbol
      • ITGB2
      • LFA-1
      • MAC-1
      • MF17
      • LCAMB
      • MFI7
      • LAD
      • CD18
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05107 # Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1.
      SIZE: 769 amino acids; 84782 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L, alpha-M, alpha-X or alpha-D. Interacts with COPS5 and RANBP9.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.DISEASE:SwissProt: P05107 # Defects in ITGB2 are the cause of leukocyte adhesion deficiency type I (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
      SIMILARITY: SwissProt: P05107 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain between 2 and 8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN b2 MONOCLONAL ANTIBODY - 2202460 2202460
      MOUSE ANTI-HUMAN INTEGRIN β2 MONOCLONAL ANTIBODY - 2469374 2469374

      References

      Reference overviewPub Med ID
      Andes virus recognition of human and Syrian hamster beta3 integrins is determined by an L33P substitution in the PSI domain.
      Valery S Matthys,Elena E Gorbunova,Irina N Gavrilovskaya,Erich R Mackow
      Journal of virology 84 2010

      Show Abstract Full Text Article
      19846530 19846530
      Alveolar type II cells from ethanol-fed rats produce a fibronectin-enriched extracellular matrix that promotes monocyte activation.
      Lou Ann S Brown, Jeffrey D Ritzenthaler, David M Guidot, Jesse Roman
      Alcohol (Fayetteville, N.Y.) 41 317-24 2007

      Show Abstract Full Text Article
      17889308 17889308
      Effects on rotavirus cell binding and infection of monomeric and polymeric peptides containing alpha2beta1 and alphaxbeta2 integrin ligand sequences
      Graham, Kate L, et al
      J Virol, 78:11786-97 (2004) 2004

      15479820 15479820
      Lipopolysaccharide induces expression of fibronectin alpha 5 beta 1-integrin receptors in human monocytic cells in a protein kinase C-dependent fashion.
      Roman, J; Ritzenthaler, JD; Boles, B; Lois, M; Roser-Page, S
      American journal of physiology. Lung cellular and molecular physiology 287 L239-49 2004

      Show Abstract
      15064224 15064224
      Growth factors induce monocyte binding to vascular smooth muscle cells: implications for monocyte retention in atherosclerosis.
      Cai, Q; Lanting, L; Natarajan, R
      American journal of physiology. Cell physiology 287 C707-14 2004

      Show Abstract
      15140748 15140748
      Oriented endocytic recycling of alpha5beta1 in motile neutrophils.
      L M Pierini, M A Lawson, R J Eddy, B Hendey, F R Maxfield
      Blood 95 2471-80 2000

      Show Abstract
      10753823 10753823
      Transcriptional regulation of the interleukin-1beta promoter via fibrinogen engagement of the CD18 integrin receptor.
      R L Perez, J D Ritzenthaler, J Roman
      American journal of respiratory cell and molecular biology 20 1059-66 1999

      Show Abstract
      10226077 10226077
      Differential modes of regulation of interleukin-1beta expression by extracellular matrices.
      J Roman, J D Ritzenthaler, R L Perez, S L Roser
      Immunology 98 228-37 1999

      Show Abstract Full Text Article
      10540221 10540221
      Dynamic imaging of neutrophil migration in three dimensions: mechanical interactions between cells and matrix.
      J T Mandeville, M A Lawson, F R Maxfield, J T Mandeville, M A Lawson, F R Maxfield
      Journal of leukocyte biology 61 188-200 1997

      Show Abstract
      9021925 9021925

      Data Sheet

      Title
      Anti-Integrin beta2, clone P4H9 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies