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MAB1959 | Anti-Integrin β1 Antibody, clone P5D2

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ELISA, FC, ICC, IHC, IP, FUNC M Purified Monoclonal Antibody
      Catalogue NumberMAB1959
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β1 Antibody, clone P5D2
      Alternate Names
      • CD29
      • MAB1959Z
      Background InformationIntegrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
      Product Information
      • Human tonsil, human skin tissue
      • A431 & HeLa Cells
      PresentationPurified immunoglobulin in 0.02M PB, 0.25M NaCl, pH 7.6, 0.1% sodium azide.
      ApplicationAnti-Integrin β1 Antibody, clone P5D2 detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IC, IH & IP.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Affects Function
      Application NotesImmunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.

      Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone fixation, no paraffin embedding).

      ELISA: A representative lot of this antibody was used in ELISA.

      Immunocytochemistry: A representative lot of this antibody clone was used in immunocytochemistry (paraformaldehyde fixation at less than 4%).

      Functional Activity Assay: A representative lot of this antibody clone was used in cell attachment assay of SV-HFO cells with a characteristic spread morphology. In the presence of function-blocking mAbs to β1 integrin (P5D2), the cells attached but no longer spread, and displayed a rounded morphology with many cytoplasmic projections (Iba, K. et al., 2000).
      Biological Information
      ImmunogenHuman keratinocytes derived from skin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityReacts with Human Integrin Beta 1.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
      Gene Symbol
      • ITGB1
      • GPIIA
      • OTTHUMP00000063731
      • OTTHUMP00000046253
      • OTTHUMP00000063733
      • VLAB
      • OTTHUMP00000063732
      • MDF2
      • FNRB
      • CD29
      • MSK12
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

      SIZE: 798 amino acids; 88465 Da

      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

      TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).

      SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceFlow Cytometry Analysis:
      4 µg of the antibody was used to detect Integrin β1 in 1x10^6 A431 cells.
      4 µg of the antibody was used to detect Integrin β1 in 1x10^6 HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN BETA 1 - 2494042 2494042
      MOUSE ANTI-HUMAN INTEGRIN BETA 1 -2595343 2595343
      MOUSE ANTI-HUMAN INTEGRIN BETA 1 -2607259 2607259
      MOUSE ANTI-HUMAN INTEGRIN BETA 1 -2685643 2685643
      MOUSE ANTI-HUMAN INTEGRIN BETA 1 -2724453 2724453
      MOUSE ANTI-HUMAN INTEGRIN BETA 1 -2763956 2763956

      References | 30 Available | See All References

      Reference overviewApplicationPub Med ID
      Substrate stress relaxation regulates cell spreading.
      Chaudhuri, O; Gu, L; Darnell, M; Klumpers, D; Bencherif, SA; Weaver, JC; Huebsch, N; Mooney, DJ
      Nature communications 6 6364 2015

      Show Abstract
      25695512 25695512
      Exogenous IGFBP-2 promotes proliferation, invasion, and chemoresistance to temozolomide in glioma cells via the integrin β1-ERK pathway.
      Han, S; Li, Z; Master, LM; Master, ZW; Wu, A
      British journal of cancer 111 1400-9 2014

      Show Abstract
      25093489 25093489
      Roles of the putative integrin-binding motif of the human metapneumovirus fusion (f) protein in cell-cell fusion, viral infectivity, and pathogenesis.
      Wei, Y; Zhang, Y; Cai, H; Mirza, AM; Iorio, RM; Peeples, ME; Niewiesk, S; Li, J
      Journal of virology 88 4338-52 2014

      Show Abstract
      24478423 24478423
      Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.
      Öhlund, D; Franklin, O; Lundberg, E; Lundin, C; Sund, M
      BMC cancer 13 154 2013

      Show Abstract
      23530721 23530721
      Adhesion, vitality and osteogenic differentiation capacity of adipose derived stem cells seeded on nitinol nanoparticle coatings.
      Strauss, S; Neumeister, A; Barcikowski, S; Kracht, D; Kuhbier, JW; Radtke, C; Reimers, K; Vogt, PM
      PloS one 8 e53309 2013

      Show Abstract
      Immunofluorescence23308190 23308190
      Rab25 regulates integrin expression in polarized colonic epithelial cells.
      Krishnan, M; Lapierre, LA; Knowles, BC; Goldenring, JR
      Molecular biology of the cell 24 818-31 2013

      Show Abstract
      Immunofluorescence23345591 23345591
      Elevated expression of syntenin in breast cancer is correlated with lymph node metastasis and poor patient survival.
      Yang, Y; Hong, Q; Shi, P; Liu, Z; Luo, J; Shao, Z
      Breast cancer research : BCR 15 R50 2013

      Show Abstract
      23786877 23786877
      Synstatin: a selective inhibitor of the syndecan-1-coupled IGF1R-αvβ3 integrin complex in tumorigenesis and angiogenesis.
      Rapraeger, Alan C
      FEBS J., (2013) 2013

      Show Abstract
      23375101 23375101
      Matrix metalloproteinase-2 cleavage of the β1 integrin ectodomain facilitates colon cancer cell motility.
      Kryczka, J; Stasiak, M; Dziki, L; Mik, M; Dziki, A; Cierniewski, C
      The Journal of biological chemistry 287 36556-66 2012

      Show Abstract
      22898815 22898815
      Loss of epidermal hypoxia-inducible factor-1α accelerates epidermal aging and affects re-epithelialization in human and mouse.
      Rezvani, HR; Ali, N; Serrano-Sanchez, M; Dubus, P; Varon, C; Ged, C; Pain, C; Cario-André, M; Seneschal, J; Taïeb, A; de Verneuil, H; Mazurier, F
      Journal of cell science 124 4172-83 2011

      Show Abstract
      22193962 22193962

      Data Sheet

      Anti-Integrin beta1, clone P5D2 - Data Sheet