A part of MilliporeSigma

MAB2252 | Anti-Integrin β1 Antibody, clone N29

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, IHC, IP, FUNC, CULT, WB M Purified Monoclonal Antibody
      Catalogue NumberMAB2252
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β1 Antibody, clone N29
      Alternate Names
      • CD29
      • MAB2252Z
      Product Information
      • Tonsil, human skin, human kidney tissue
      • U251 cell lysate
      PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
      ApplicationThis Anti-Integrin β1 Antibody, clone N29 is validated for use in FC, IH, IP, FUNC, CULT, WB for the detection of Integrin β1.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Affects Function
      • Cell Culture
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50-2,000 dilution from a representative lot detected Integrin β1 in the membrane of tubule epithelial cells and cells of the glomeruli in human kidney tissue, membrane of alveolar cells, endothelial cells, and bronchiole epithelial cells of human lung tissue.

      Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.

      Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.

      Flow Cytometry: A representative lot of this antibody clone was used in flow cytometry.

      Functional Activity Assay: A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenJurkat T-leukemic cell line
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityReacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes an epitope cluster distinct from MAB2250 and MAB2251(Wilkins et al., 1996).
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAMB2
      • S-laminin
      • LAMS
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55268 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
      SIZE: 1798 amino acids; 195981 Da
      SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-2 is a subunit of laminin-3 (S-laminin), laminin-4 (S- merosin), and laminin-7 (KS-laminin).
      SUBCELLULAR LOCATION: Secreted, extracellular space. Note=S- laminin is concentrated in the synaptic cleft of the neuromuscular junction.
      DOMAIN: SwissProt: P55268 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular.
      DISEASE: SwissProt: P55268 # Defects in LAMB2 are the cause of Pierson syndrome [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome. & Defects in LAMB2 are a cause of congenital nephrotic syndrome [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities.
      SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain.
      Molecular Weight88 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: 0.5 µg/mL of the antibody detected Integrin β1 in 10 µg of U251 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN β1 -2527479 2527479
      MOUSE ANTI-HUMAN INTEGRIN β1 -2682557 2682557
      MOUSE ANTI-HUMAN INTEGRIN 1 -2814542 2814542


      Reference overviewApplicationPub Med ID
      Endothelial destabilization by angiopoietin-2 via integrin β1 activation.
      Hakanpaa, L; Sipila, T; Leppanen, VM; Gautam, P; Nurmi, H; Jacquemet, G; Eklund, L; Ivaska, J; Alitalo, K; Saharinen, P
      Nature communications 6 5962 2015

      Show Abstract
      Immunofluorescence25635707 25635707
      Compensatory redistribution of neuroligins and N-cadherin following deletion of synaptic β1-integrin.
      Steven Mortillo,Alice Elste,Yongchao Ge,Shekhar B Patil,Kuangfu Hsiao,George W Huntley,Ronald L Davis,Deanna L Benson
      The Journal of comparative neurology 520 2012

      Show Abstract
      22488504 22488504
      Distinct recycling of active and inactive β1 integrins.
      Arjonen, A; Alanko, J; Veltel, S; Ivaska, J
      Traffic (Copenhagen, Denmark) 13 610-25 2012

      Show Abstract
      22222055 22222055
      Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration.
      Mai, A; Veltel, S; Pellinen, T; Padzik, A; Coffey, E; Marjomäki, V; Ivaska, J
      The Journal of cell biology 194 291-306 2011

      Show Abstract
      21768288 21768288
      Mammary-derived growth inhibitor (MDGI) interacts with integrin ?-subunits and suppresses integrin activity and invasion.
      J Nevo,A Mai,S Tuomi,T Pellinen,O T Pentikäinen,P Heikkilä,J Lundin,H Joensuu,P Bono,J Ivaska
      Oncogene 29 2010

      Show Abstract
      20802519 20802519
      Mammary-derived growth inhibitor alters traffic of EGFR and induces a novel form of cetuximab resistance.
      Jonna Nevo,Elina Mattila,Teijo Pellinen,Daniel L Yamamoto,Henri Sara,Kristiina Iljin,Olli Kallioniemi,Petri Bono,Päivi Heikkilä,Heikki Joensuu,Anni Wärri,Johanna Ivaska
      Clinical cancer research : an official journal of the American Association for Cancer Research 15 2009

      Show Abstract
      19825952 19825952
      Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins.
      Pellinen, T; Arjonen, A; Vuoriluoto, K; Kallio, K; Fransen, JA; Ivaska, J
      The Journal of cell biology 173 767-80 2006

      Show Abstract
      16754960 16754960
      Matrix metalloproteinase 1 interacts with neuronal integrins and stimulates dephosphorylation of Akt.
      Conant, K; St Hillaire, C; Nagase, H; Visse, R; Gary, D; Haughey, N; Anderson, C; Turchan, J; Nath, A
      The Journal of biological chemistry 279 8056-62 2004

      Show Abstract
      14679206 14679206
      Integrin shedding as a mechanism of cellular adaptation during cardiac growth.
      Edie C Goldsmith, Wayne Carver, Alex McFadden, Jack G Goldsmith, Robert L Price, Mark Sussman, Beverly H Lorell, Garth Cooper, Thomas K Borg, Edie C Goldsmith, Wayne Carver, Alex McFadden, Jack G Goldsmith, Robert L Price, Mark Sussman, Beverly H Lorell, Garth Cooper, Thomas K Borg
      American journal of physiology. Heart and circulatory physiology 284 H2227-34 2003

      Show Abstract
      12573995 12573995
      Control of beta1 integrin function. Localization of stimulatory epitopes.
      Wilkins, J A, et al.
      J. Biol. Chem., 271: 3046-51 (1996) 1996

      Show Abstract
      8621699 8621699

      Data Sheet


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