A part of MilliporeSigma

MAB1976-AF555 | Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate

100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, Rb, B, Ca, Ch, H, Mk, Po ICC M AlexaFluor®555 Monoclonal Antibody
      Catalogue NumberMAB1976-AF555
      DescriptionAnti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate
      Alternate Names
      • Integrin alpha-V
      • Vitronectin receptor subunit alpha
      • CD51
      • Integrin alpha-V heavy chain
      • Integrin alpha-V light chain
      • Integrin αVβ3
      Background InformationIntegrin alpha-V (CD51, Vitronectin receptor subunit alpha; UniProt P06756) and Integrin beta-3 (CD61, GPIIIa, Platelet membrane glycoprotein IIIa; UniProt P05106) are encoded by the ITGAV (MSK8, VNRA; Gene ID 3685) and ITGB3 (GP3A; Gene ID 3690) gene, respectively, in human. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and a beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue. Due to its involvement in angiogenesis, integrin alphaVbeta3 is among of the most studied integrin receptors.
      Product Information
      PresentationPurified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
      ApplicationAnti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate is an antibody against Integrin αVβ3 for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin αVβ3 in HeLa cells.
      The unconjugated antibody (Cat. No. MAB1976) is shown to be suitable also for functional assay, flow cytometry, immunofluorescence, immunohistochemistry, and immunoprecipitation applications.
      Biological Information
      ImmunogenPurified adhesion receptor from the human melanoma cell line M21 (Cheresh, D.A., and Spiro R.C. (1987). J. Biol. Chem. 262(36):17703-11).
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityReacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, D.A., and Spiro R.C. (1987). J Biol Chem. 262(36):17703-11; Cheresh, D.A. (1987). Proc. Natl. Acad. Sci. U.S.A. 84(18):6471-6475). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, P.C., et al. (1994). Science. 264(5158):569-571). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
      Species Reactivity
      • Avian
      • Rabbit
      • Bovine
      • Canine
      • Chicken
      • Human
      • Monkey
      • Pig
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITGAV
      • MSK8
      • VNRA
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~116 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin αVβ3 in HepG2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

      Related Products & Applications

      Related Products

      Catalog Number Description  
      AB1923 Anti-Integrin αV Antibody, cytoplasmic domain Show Pricing & Availability
      AB1930 Anti-Integrin alphaV Antibody, CT, Intracellular Show Pricing & Availability
      CBL490 Anti-Integrin αV Antibody, clone 13C2 Show Pricing & Availability
      CBL490P Anti-Integrin αV Antibody, clone 13C2, Phycoerythrin conjugated Show Pricing & Availability
      CBL544 Anti-Integrin αVβ3 Antibody, clone 23C6 Show Pricing & Availability
      FCMAB282F Milli-Mark™ Anti-Integrin αVβ3 Antibody, clone LM609-FITC Show Pricing & Availability
      MAB1876 Anti-Integrin αVβ3 Antibody, clone 27.1 (VNR-1) Show Pricing & Availability
      MAB1953 Anti-Integrin αV Antibody, clone P3G8 Show Pricing & Availability
      MAB1953Z Anti-Integrin αV Antibody, clone P3G8, azide free Show Pricing & Availability
      MAB1961 Anti-Integrin αVβ5 Antibody, clone P1F6 Show Pricing & Availability
      MAB1961F Anti-Integrin αVβ5 Antibody, clone P1F6, FITC conjugated Show Pricing & Availability
      MAB1961H Anti-Integrin αVβ5 Antibody, clone P1F6, Phycoerythrin conjugated Show Pricing & Availability
      MAB1961X Anti-Integrin αVβ5 Antibody, clone P1F6, Alexa Fluor® 488 conjugated Show Pricing & Availability
      MAB1961Z Anti-Integrin αVβ5 Antibody, clone P1F6, azide free Show Pricing & Availability
      MAB1976 Anti-Integrin αVβ3 Antibody, clone LM609 Show Pricing & Availability
      MAB1976B Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated Show Pricing & Availability
      MAB1976F Anti-Integrin αVβ3 Antibody, clone LM609, FITC conjugated Show Pricing & Availability
      MAB1976H Anti-Integrin αVβ3 Antibody, clone LM609, Phycoerythrin conjugated Show Pricing & Availability
      MAB1976X Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor®488 conjugated Show Pricing & Availability
      MAB1976Z Anti-Integrin αVβ3 Antibody, clone LM609, Azide Free Show Pricing & Availability
      MAB1978 Anti-Integrin αV Antibody, clone LM142 Show Pricing & Availability
      MAB1980 Anti-Integrin αV Antibody, clone M9 Show Pricing & Availability
      MAB2019Z Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free Show Pricing & Availability
      MAB2021Z Anti-Integrin αV Antibody, extracellular, clone AV1, azide free Show Pricing & Availability
      MAB2074Z Anti-Integrin αVβ6 Antibody, clone E7P6, azide free Show Pricing & Availability
      MAB2077Z Anti-Integrin αVβ6 Antibody, clone 10D5, azide free Show Pricing & Availability
      MABT201 Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51 Show Pricing & Availability
      MABT207 Anti-Integrin alpha-V Antibody, clone 272-17E6 (Azide free) Show Pricing & Availability

      View Gene Related Products for this Target


      Life Science Research > Antibodies and Assays > Primary Antibodies