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AB1928 | Anti-Integrin α5 Antibody, CT, Intracellular

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, M, Ht, R ELISA, ICC, IF, IHC, IP, RIA, WB Rb Serum Polyclonal Antibody
      Catalogue NumberAB1928
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin α5 Antibody, CT, Intracellular
      Alternate Names
      • CD49e
      • Fibronectin Receptor alpha Subunit
      Background InformationIntegrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.
      Product Information
      • Mouse 3T3 fibroblasts Skin (Basement Membrane).
      PresentationRabbit polyclonal antiserum in buffer containing 0.05% sodium azide.
      ApplicationAnti-Integrin α5 Antibody, C-terminus, Intracellular detects level of Integrin α5 & has been published & validated for use in ELISA, IC, IF, IH, IP, RIA & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Radioimmunoassay
      • Western Blotting
      Application NotesWestern Blot Analysis:
      1:1000 dilution of a previous lot was used.

      1:500-1:2000 dilution of a previous lot was used in ELISA.

      Recommended use of 5 μL of antibody for 5x106 cells.

      1:1000 dilution of a previous lot was used in tissue staining; suggested for use on acetone fixed tissue only.

      1:1000 of a previous lot was used in immunocytochemistry.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha5 integrin subunit.
      EpitopeC-terminus, intracellular
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityAntibody recognizes integrin alpha5. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. No cross-reactivity to alpha1, alpha2, alpha3, alpha4, alpha6 or alphaV. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus, intracellular.
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Hamster
      • Rat
      Species Reactivity NoteHuman, mouse, rat, hamster, and chicken. Reactivity with other species has not been determined.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq]
      Gene Symbol
      • ITGA5
      • VLA-5
      • CD49e
      • VLA5A
      • FNRA
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1049 amino acids; 114536 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Molecular Weight114 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HUVEC lysates.

      Western Blot Analysis: 1:1000 dilution of this lot detected integrin alpha 5 on 10 μg of HUVEC lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Integrin #945;5, C-terminus, Intracellular - 2136559 2136559
      Anti-Integrin #945;5, C-terminus, Intracellular - NG1827386 NG1827386
      Anti-Integrin 5, C-terminus, Intracellular - 2010656 2010656
      Anti-Integrin 5, C-terminus, Intracellular - 2362877 2362877
      Anti-Integrin 5, C-terminus, Intracellular - JC1628131 JC1628131
      Anti-Integrin 5, C-terminus, Intracellular - JC1634169 JC1634169
      Anti-Integrin 5, C-terminus, Intracellular - NG1713411 NG1713411
      Anti-Integrin 5, C-terminus, Intracellular - NG1721770 NG1721770
      Anti-Integrin 5, C-terminus, Intracellular - NG1758231 NG1758231
      Anti-Integrin 5, C-terminus, Intracellular - NG1780617 NG1780617
      Anti-Integrin 5, C-terminus, Intracellular - NG1858875 NG1858875
      Anti-Integrin alpha5, C-terminus, Intracellular - LV1471005 LV1471005
      Anti-Integrin α5, C-terminus, -2654608 2654608
      Anti-Integrin α5, C-terminus, -2802787 2802787
      Anti-Integrin α5, C-terminus, Intracellular - 2398846 2398846
      Anti-Integrin α5, C-terminus, Intracellular - 2458952 2458952
      Anti-Integrin α5, C-terminus, Intracellular -2483196 2483196
      Anti-Integrin α5, C-terminus, Intracellular -2581329 2581329
      Anti-Integrin α5, C-terminus, Intracellular -2620166 2620166
      Anti-Integrin α5, C-terminus, Intracellular -2660172 2660172
      Anti-Integrin α5, C-terminus, Intracellular -2698380 2698380
      Anti-Integrin α5, C-terminus, Intracellular -2722858 2722858
      Anti-Integrin α5, C-terminus, Intracellular -2776885 2776885

      References | 64 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Dexamethasone-Mediated Activation of Fibronectin Matrix Assembly Reduces Dispersal of Primary Human Glioblastoma Cells.
      Shannon, S; Vaca, C; Jia, D; Entersz, I; Schaer, A; Carcione, J; Weaver, M; Avidar, Y; Pettit, R; Nair, M; Khan, A; Foty, RA
      PloS one 10 e0135951 2015

      Show Abstract
      26284619 26284619
      Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
      Poitelon, Y; Bogni, S; Matafora, V; Della-Flora Nunes, G; Hurley, E; Ghidinelli, M; Katzenellenbogen, BS; Taveggia, C; Silvestri, N; Bachi, A; Sannino, A; Wrabetz, L; Feltri, ML
      Nature communications 6 8303 2015

      Show Abstract
      Immunohistochemistry26383514 26383514
      Poly(vinyl alcohol)/gelatin Hydrogels Cultured with HepG2 Cells as a 3D Model of Hepatocellular Carcinoma: A Morphological Study.
      Moscato, S; Ronca, F; Campani, D; Danti, S
      Journal of functional biomaterials 6 16-32 2015

      Show Abstract
      25590431 25590431
      Directional cell movement through tissues is controlled by exosome secretion.
      Sung, BH; Ketova, T; Hoshino, D; Zijlstra, A; Weaver, AM
      Nature communications 6 7164 2015

      Show Abstract
      25968605 25968605
      Exclusion of integrins from CNS axons is regulated by Arf6 activation and the AIS.
      Franssen, EH; Zhao, RR; Koseki, H; Kanamarlapudi, V; Hoogenraad, CC; Eva, R; Fawcett, JW
      The Journal of neuroscience : the official journal of the Society for Neuroscience 35 8359-75 2015

      Show Abstract
      26019348 26019348
      Secreted protein acidic and rich in cysteine internalization and its age-related alterations in skeletal muscle progenitor cells.
      Nakamura, K; Yamanouchi, K; Nishihara, M
      Aging cell 13 175-84 2014

      Show Abstract
      24245505 24245505
      The cancer glycocalyx mechanically primes integrin-mediated growth and survival.
      Paszek, MJ; DuFort, CC; Rossier, O; Bainer, R; Mouw, JK; Godula, K; Hudak, JE; Lakins, JN; Wijekoon, AC; Cassereau, L; Rubashkin, MG; Magbanua, MJ; Thorn, KS; Davidson, MW; Rugo, HS; Park, JW; Hammer, DA; Giannone, G; Bertozzi, CR; Weaver, VM
      Nature 511 319-25 2014

      Show Abstract
      25030168 25030168
      Recombinant fibronectin matrix mimetics specify integrin adhesion and extracellular matrix assembly.
      Roy, DC; Hocking, DC
      Tissue engineering. Part A 19 558-70 2013

      Show Abstract
      23020251 23020251
      Integrin α5β1 plays a critical role in resistance to temozolomide by interfering with the p53 pathway in high-grade glioma.
      Janouskova, H; Maglott, A; Leger, DY; Bossert, C; Noulet, F; Guerin, E; Guenot, D; Pinel, S; Chastagner, P; Plenat, F; Entz-Werle, N; Lehmann-Che, J; Godet, J; Martin, S; Teisinger, J; Dontenwill, M
      Cancer research 72 3463-70 2012

      Show Abstract
      Western Blotting22593187 22593187
      Perlecan domain V is upregulated in human brain arteriovenous malformation and could mediate the vascular endothelial growth factor effect in lesional tissue.
      Kahle, MP; Lee, B; Pourmohamad, T; Cunningham, A; Su, H; Kim, H; Chen, Y; McCulloch, CE; Barbaro, NM; Lawton, MT; Young, WL; Bix, GJ
      Neuroreport 23 627-30 2012

      Show Abstract
      Western Blotting22643235 22643235

      Data Sheet

      Anti-Integrin alpha5, C-terminus, intracellular - Data Sheet

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