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MAB1999 | Anti-Integrin α5β1 Antibody, clone HA5

MAB1999
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Po FC, IP M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1999
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin α5β1 Antibody, clone HA5
      Alternate Names
      • VLA-5
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin from Protein A chromatography. Liquid in 0.02 M PB pH 7.6, 0.25 M NaCl, 0.1% sodium azide.
      Applications
      ApplicationAnti-Integrin α5β1 Antibody, clone HA5 detects level of Integrin α5β1 & has been published & validated for use in FC & IP.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      Application NotesImmunoprecipitation of VLA-5 integrins from lysate of 10E6 cell equivalent requires 2 μg of MAB1999.

      Flow cytometry: 10 μg/mL

      Does not detect VLA-5 by Western blot.

      MAB1999 does not affect VLA-5 mediated cell adhesion to fibronectin.

      Optimal working dilutions must be determined by end user.
      Biological Information
      CloneHA5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with the fibronectin receptor VLA-5 (alpha-5beta-1) integrin complex.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Pig
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
      Gene Symbol
      • ITGA5
      • VLA-5
      • CD49e
      • VLA5A
      • FNRA
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1049 amino acids; 114536 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN VLA-5 (#945;5#946;1) INTEGRIN (FIBRONECTIN RECEPTOR) NON-BLOCKING MONOCLONAL ANTIBODY - 2118019 2118019
      MOUSE ANTI-HUMAN VLA-5 (a5b1) INTEGRIN (FIBRONECTIN RECEPTOR) NON-BLOCKING MONOCLONAL ANTIBODY - 2022034 2022034
      MOUSE ANTI-HUMAN VLA-5 (a5b1) INTEGRIN (FIBRONECTIN RECEPTOR) NON-BLOCKING MONOCLONAL ANTIBODY - 2179336 2179336
      MOUSE ANTI-HUMAN VLA-5 (a5b1) INTEGRIN (FIBRONECTIN RECEPTOR) NON-BLOCKING MONOCLONAL ANTIBODY - 2281654 2281654
      MOUSE ANTI-HUMAN VLA-5 (α5β1) INTEGRIN (FIBRONECTIN RECEPTOR) NON-BLOCKING MONOCLONAL ANTIBODY - 2379352 2379352
      MOUSE ANTI-HUMAN VLA-5 (α5β1) INTEGRIN (FIBRONECTIN RECEPTOR) NON-BLOCKING MONOCLONAL ANTIBODY - 2433460 2433460
      MOUSE ANTI-HUMAN VLA-5 (α5β1) INTEGRIN (FIBRONECTIN RECEPTOR) -2500566 2500566
      MOUSE ANTI-HUMAN VLA-5 (α5β1) INTEGRIN (FIBRONECTIN RECEPTOR) -2694555 2694555
      MOUSE ANTI-HUMAN VLA-5 (α5β1) INTEGRIN (FIBRONECTIN RECEPTOR) -2757125 2757125

      References | 22 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Regulation of ionizing radiation-induced adhesion of breast cancer cells to fibronectin by alpha5beta1 integrin.
      Lee, SH; Cheng, H; Yuan, Y; Wu, S
      Radiation research 181 650-8 2014

      Show Abstract
      24785587 24785587
      Tropism-modified AAV vectors overcome barriers to successful cutaneous therapy.
      Sallach, J; Di Pasquale, G; Larcher, F; Niehoff, N; Rübsam, M; Huber, A; Chiorini, J; Almarza, D; Eming, SA; Ulus, H; Nishimura, S; Hacker, UT; Hallek, M; Niessen, CM; Büning, H
      Molecular therapy : the journal of the American Society of Gene Therapy 22 929-39 2014

      Show Abstract
      24468915 24468915
      High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress.
      Shin, ES; Huang, Q; Gurel, Z; Sorenson, CM; Sheibani, N
      PloS one 9 e103148 2014

      Show Abstract
      25068294 25068294
      Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress.
      Zhao, Y; Wang, S; Sorenson, CM; Teixeira, L; Dubielzig, RR; Peters, DM; Conway, SJ; Jefcoate, CR; Sheibani, N
      Molecular and cellular biology 33 4225-40 2013

      Show Abstract
      Dot BlotMouse23979599 23979599
      Role of MMP-2 in the regulation of IL-6/Stat3 survival signaling via interaction with α5β1 integrin in glioma.
      Kesanakurti, D; Chetty, C; Dinh, DH; Gujrati, M; Rao, JS
      Oncogene 32 327-40 2013

      Show Abstract
      22349830 22349830
      Regulation of integrin endocytic recycling and chemotactic cell migration by syntaxin 6 and VAMP3 interaction.
      Riggs, KA; Hasan, N; Humphrey, D; Raleigh, C; Nevitt, C; Corbin, D; Hu, C
      Journal of cell science 125 3827-39 2012

      Show Abstract
      22573826 22573826
      Gene transfer into human cord blood-derived CD34(+) cells by adeno-associated viral vectors.
      Schuhmann NK, Pozzoli O, Sallach J, Huber A, Avitabile D, Perabo L, Rappl G, Capogrossi MC, Hallek M, Pesce M, Büning H
      Exp Hematol 38 707-17. Epub 2010 May 4. 2010

      Show Abstract
      20447441 20447441
      Different strains of Theiler's murine encephalomyelitis virus antagonize different sites in the type I interferon pathway.
      Stavrou S, Feng Z, Lemon SM, Roos RP
      J Virol 2010

      Show Abstract
      20610716 20610716
      Autocrine fibronectin directs matrix assembly and crosstalk between cell-matrix and cell-cell adhesion in vascular endothelial cells.
      Cseh, B; Fernandez-Sauze, S; Grall, D; Schaub, S; Doma, E; Van Obberghen-Schilling, E
      Journal of cell science 123 3989-99 2010

      Show Abstract
      20980391 20980391
      Tumor necrosis factor-induced neutrophil adhesion occurs via sphingosine kinase-1-dependent activation of endothelial {alpha}5{beta}1 integrin.
      Sun WY, Pitson SM, Bonder CS
      Am J Pathol 177 436-46. Epub 2010 Jun 3. 2010

      Show Abstract Full Text Article
      20522645 20522645

      Data Sheet

      Title
      Anti-Integrin alpha5beta1, clone HA5 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies