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MABE912 | Anti-Ikaros Antibody, clone 4E9

MABE912
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, FC, ChIP, ChIP-seq M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE912
      DescriptionAnti-Ikaros Antibody, clone 4E9
      Alternate Names
      • DNA-binding protein Ikaros
      • Ikaros family zinc finger protein 1
      • Lymphoid transcription factor LyF-1
      Background InformationIkaros, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. Ikaros has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Ikaros Antibody, clone 4E9 is validated for use in Western Blotting and Flow Cytometry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Ikaros.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      Application NotesFlow Cytometry Analsysis: 1 µg of a representative lot of this antibody detected Ikaros in Mouse thymocytes

      Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP. See Zhang et al. (2012).

      ChIP-seq Analsysis: A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP-seq. See Zhang et al. (2012).
      Biological Information
      ImmunogenRecombinant protein corresponding to Human Ikaros.
      Clone4E9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IKZF1
      • IK1
      • IKAROS
      • LYF1
      • ZNFN1A1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~60 kDa observed. Uncharacterized bands may be observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in a Jurkat cell lysate.

      Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Ikaros in a Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ikaros, clone 4E9 Q2478121
      Anti-Ikaros, clone 4E9 -2744119 2744119
      Anti-Ikaros, clone 4E9 -2806006 2806006

      References

      Reference overviewPub Med ID
      Harnessing of the nucleosome-remodeling-deacetylase complex controls lymphocyte development and prevents leukemogenesis.
      Zhang, J; Jackson, AF; Naito, T; Dose, M; Seavitt, J; Liu, F; Heller, EJ; Kashiwagi, M; Yoshida, T; Gounari, F; Petrie, HT; Georgopoulos, K
      Nature immunology 13 86-94 2012

      Show Abstract
      22080921 22080921

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies