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MABN92 | Anti-Iba1/AIF1 Antibody

MABN92
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABN92
      DescriptionAnti-Iba1/AIF1 Antibody
      Alternate Names
      • Allograft inflammatory factor 1
      • AIF-1
      • Ionized calcium-binding adapter molecule 1
      • Protein G1
      Background InformationThe allograft inflammatory factor-1 (AIF-1) is a 17 kDa interferon-gamma (IFN-gamma) inducible Ca(2+)-binding EF-hand protein that is encoded within the HLA class III genomic region. AIF-1 is known to be identical with ionized Ca(2+)-binding adaptor 1, microglia response factor-1, and daintain. AIF-1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and the activation and function of macrophages. It is also shown to be expressed in macrophages and microglial cells in autominnume disesases such as experimental autoimmune encephalomyelitis, and neuritis and uveitis models.
      References
      Product Information
      FormatPurified
      Control
      • Human thymus tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Iba1/AIF1 Antibody is an antibody against Iba1/AIF1 for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of mouse thymus tissue lysate.

      Western Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of rat thymus tissue lysate.

      Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Iba/AIF1 in human spleen tissue.
      Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Iba/AIF1 in rat spleen tissue
      Biological Information
      ImmunogenLinear peptide corresponding to human Iba1/AIF1.
      Clone20A12.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • AIF1
      • G1
      • IBA1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

      SUBUNIT STRUCTURE: Homodimer. Monomer. Interacts with LCP1.

      SUBCELLULAR LOCATION: Cytoplasm > cytoskeleton. Cell projection > ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

      TISSUE SPECIFICITY: Detected in T-lymphocytes and peripheral blood mononuclear cells.
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated in Western Blot in human thymus tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Iba1/AIF1 in 10 µg of human thymus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Iba1/AIF1 2475914
      Anti-Iba1/AIF1 - 2309350 2309350
      Anti-Iba1/AIF1 - 2383608 2383608
      Anti-Iba1/AIF1 - 1963613 1963613
      Anti-Iba1/AIF1 - 1979269 1979269
      Anti-Iba1/AIF1 - 1987496 1987496
      Anti-Iba1/AIF1 - 2010878 2010878
      Anti-Iba1/AIF1 - 2023184 2023184
      Anti-Iba1/AIF1 - 2055302 2055302
      Anti-Iba1/AIF1 - 2090498 2090498
      Anti-Iba1/AIF1 - 2172784 2172784
      Anti-Iba1/AIF1 - 2251774 2251774
      Anti-Iba1/AIF1 - 2279268 2279268
      Anti-Iba1/AIF1 - 2491261 2491261
      Anti-Iba1/AIF1 - NG1945600 NG1945600
      Anti-Iba1/AIF1 - NRG1920225 NRG1920225
      Anti-Iba1/AIF1 -2549436 2549436
      Anti-Iba1/AIF1 -2562012 2562012
      Anti-Iba1/AIF1 -2594426 2594426
      Anti-Iba1/AIF1 -2625394 2625394
      Anti-Iba1/AIF1 -2659641 2659641
      Anti-Iba1/AIF1 -2695332 2695332
      Anti-Iba1/AIF1 -2733301 2733301
      Anti-Iba1/AIF1 -2787670 2787670
      Anti-Iba1/AIF1 Monoclonal Antibody_2804252 2804252

      References

      Reference overviewPub Med ID
      Macrophage Ablation Reduces M2-Like Populations and Jeopardizes Tumor Growth in a MAFIA-Based Glioma Model.
      Gabrusiewicz, K; Hossain, MB; Cortes-Santiago, N; Fan, X; Kaminska, B; Marini, FC; Fueyo, J; Gomez-Manzano, C
      Neoplasia (New York, N.Y.) 17 374-84 2015

      Show Abstract
      25925380 25925380
      Role of spinal P2Y6 and P2Y11 receptors in neuropathic pain in rats: possible involvement of glial cells.
      Barragán-Iglesias, P; Pineda-Farias, JB; Cervantes-Durán, C; Bravo-Hernández, M; Rocha-González, HI; Murbartián, J; Granados-Soto, V
      Molecular pain 10 29 2014

      Show Abstract
      24886406 24886406
      Striking reduction of amyloid plaque burden in an Alzheimer's mouse model after chronic administration of carmustine.
      Hayes, CD; Dey, D; Palavicini, JP; Wang, H; Patkar, KA; Minond, D; Nefzi, A; Lakshmana, MK
      BMC medicine 11 81 2013

      Show Abstract
      23531149 23531149
      Rod microglia: elongation, alignment, and coupling to form trains across the somatosensory cortex after experimental diffuse brain injury.
      Ziebell, JM; Taylor, SE; Cao, T; Harrison, JL; Lifshitz, J
      Journal of neuroinflammation 9 247 2012

      Show Abstract
      23111107 23111107

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies