MABN92 | Anti-Iba1/AIF1 Antibody

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IHC M Purified Monoclonal Antibody
      Catalogue NumberMABN92
      DescriptionAnti-Iba1/AIF1 Antibody
      Alternate Names
      • Allograft inflammatory factor 1
      • AIF-1
      • Ionized calcium-binding adapter molecule 1
      • Protein G1
      Background InformationThe allograft inflammatory factor-1 (AIF-1) is a 17 kDa interferon-gamma (IFN-gamma) inducible Ca(2+)-binding EF-hand protein that is encoded within the HLA class III genomic region. AIF-1 is known to be identical with ionized Ca(2+)-binding adaptor 1, microglia response factor-1, and daintain. AIF-1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and the activation and function of macrophages. It is also shown to be expressed in macrophages and microglial cells in autominnume disesases such as experimental autoimmune encephalomyelitis, and neuritis and uveitis models.
      Product Information
      • Human thymus tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Iba1/AIF1 Antibody is an antibody against Iba1/AIF1 for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of mouse thymus tissue lysate.

      Western Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of rat thymus tissue lysate.

      Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Iba/AIF1 in human spleen tissue.
      Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Iba/AIF1 in rat spleen tissue
      Biological Information
      ImmunogenLinear peptide corresponding to human Iba1/AIF1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • AIF1
      • G1
      • IBA1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

      SUBUNIT STRUCTURE: Homodimer. Monomer. Interacts with LCP1.

      SUBCELLULAR LOCATION: Cytoplasm > cytoskeleton. Cell projection > ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

      TISSUE SPECIFICITY: Detected in T-lymphocytes and peripheral blood mononuclear cells.
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated in Western Blot in human thymus tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Iba1/AIF1 in 10 µg of human thymus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Iba1/AIF1 2475914
      Anti-Iba1/AIF1 - 2309350 2309350
      Anti-Iba1/AIF1 - 2383608 2383608
      Anti-Iba1/AIF1 - 1963613 1963613
      Anti-Iba1/AIF1 - 1979269 1979269
      Anti-Iba1/AIF1 - 1987496 1987496
      Anti-Iba1/AIF1 - 2010878 2010878
      Anti-Iba1/AIF1 - 2023184 2023184
      Anti-Iba1/AIF1 - 2055302 2055302
      Anti-Iba1/AIF1 - 2090498 2090498


      Reference overviewPub Med ID
      Macrophage Ablation Reduces M2-Like Populations and Jeopardizes Tumor Growth in a MAFIA-Based Glioma Model.
      Gabrusiewicz, K; Hossain, MB; Cortes-Santiago, N; Fan, X; Kaminska, B; Marini, FC; Fueyo, J; Gomez-Manzano, C
      Neoplasia (New York, N.Y.)  17  374-84  2015

      Show Abstract
      25925380 25925380
      Role of spinal P2Y6 and P2Y11 receptors in neuropathic pain in rats: possible involvement of glial cells.
      Barragán-Iglesias, P; Pineda-Farias, JB; Cervantes-Durán, C; Bravo-Hernández, M; Rocha-González, HI; Murbartián, J; Granados-Soto, V
      Molecular pain  10  29  2014

      Show Abstract
      24886406 24886406
      Striking reduction of amyloid plaque burden in an Alzheimer's mouse model after chronic administration of carmustine.
      Hayes, CD; Dey, D; Palavicini, JP; Wang, H; Patkar, KA; Minond, D; Nefzi, A; Lakshmana, MK
      BMC medicine  11  81  2013

      Show Abstract
      23531149 23531149
      Rod microglia: elongation, alignment, and coupling to form trains across the somatosensory cortex after experimental diffuse brain injury.
      Ziebell, JM; Taylor, SE; Cao, T; Harrison, JL; Lifshitz, J
      Journal of neuroinflammation  9  247  2012

      Show Abstract
      23111107 23111107

      Related Products & Applications

      Related Products

      Catalog Number Description  
      ABN67 Anti-AIF-1 Antibody Show Pricing & Availability
      MABN92-AF488 Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor® 488 Conjugate Show Pricing & Availability
      MABN92-AF555 Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor® 555 Conjugate Show Pricing & Availability
      MABN92-AF647 Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor® 647 Conjugate Show Pricing & Availability


      Life Science Research > Antibodies and Assays > Primary Antibodies