A part of MilliporeSigma

09-412 | Anti-ISG15/UCRP Antibody

09-412
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number09-412
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-ISG15/UCRP Antibody
      OverviewISG15 (also called ubiquitin cross-reactive protein (UCRP) due to its cross-reactivity with ubiquitin antibodies) was originally identified as an interferon stimulated gene (ISG) whose expression is highly induced upon interferon treatment. ISG15 contains two ubiquitin homology domains in tandem and shows ~30% identity to ubiquitin. Unlike ubiquitin, that is almost 100% homologous across species, ISG15 is only conserved in dispersed regions. Homology among the five identified mammalian ISG15 proteins is only about 47%. Like other Ubls, ISG15 is synthesized as a 17-kDa precursor form and processed to 15- kDa protein by a specific protease in order to expose di-glycine residues at the carboxyl terminal which is critical for subsequent conjugation to target proteins.
      References
      Product Information
      FormatPurified
      Presentation100 µL of purified by salt precipitation and peptide immunochromatography in PBS containing 0.01 M sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
      Applications
      ApplicationDetect ISG15/UCRP using this Anti-ISG15/UCRP Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to residues 85-101 of human ISG15.
      HostRabbit
      SpecificityAntibody 09-412 recognizes several members of the ubiquitin-like (Ubl) family including ubiquitin, SUMO-1, SUMO-2, SUMO-3, Nedd8, FAT10, Ubl5, Urm1 and Fub1. This antibody shows no cross-reactivity with any of these Ubls in recombinant form upon Western blotting under the conditions used.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryG1P2 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM]
      Gene Symbol
      • ISG15
      • UCRP
      • IFI15
      • G1P2
      Purification MethodSalt precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05161 # In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy (By similarity).
      SIZE: 165 amino acids; 17888 Da
      SUBUNIT: Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation.
      SUBCELLULAR LOCATION: Cytoplasm. Secreted. Note=UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
      TISSUE SPECIFICITY: Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
      SIMILARITY: SwissProt: P05161 ## Contains 2 ubiquitin-like domains.
      Molecular Weight18 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of shipment for up to 12 months.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ISG15/UCRP (Rabbit polyclonal IgG) - 1948802 1948802
      Anti-ISG15/UCRP (Rabbit polyclonal IgG) - 2198965 2198965
      Anti-ISG15/UCRP - 0611045512 0611045512

      References

      Reference overviewPub Med ID
      Antiviral actions of interferons
      Samuel, C E
      Clin Microbiol Rev, 14:778-809, table of contents (2001) 2001

      11585785 11585785
      A gene in the chromosomal region 3p21 with greatly reduced expression in lung cancer is similar to the gene for ubiquitin-activating enzyme
      Kok, K, et al
      Proc Natl Acad Sci USA, 90:6071-5 (1993) 1993

      8327486 8327486