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ABS613 | Anti-ISCU Antibody

ABS613
150 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IHC, IF Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABS613
      DescriptionAnti-ISCU Antibody
      Alternate Names
      • Iron-sulfur cluster assembly enzyme ISCU, mitochondrial
      • NifU-like N-terminal domain-containing protein
      • NifU-like protein
      • ISCU
      Background InformationThe enzyme currently named Iron-sulfur cluster assembly enzyme ISCU, mitochondrial, or in earlier literature NifU-like N-terminal domain-containing protein or NifU-like protein, and encoded by the human gene named ISCU or NIFUN, is a biosynthetic enzyme involved in the assembly of iron-sulfur proteins. There are two isoforms in mammals. Isoform 1 is localizes to the mitochondria, whereas isoform 2 localizes to the cytoplasm. ISCU is expressed in a variety of tissues including brain, heart, skeletal muscle, lung and kidney. Iron-sulfur proteins are proteins characterized by the presence of iron-sulfur clusters containing sulfide-linked centers in variable oxidation states. Iron-sulfur cluster proteins are best known for their role in the oxidation-reduction reactions of mitochondrial electron transport. Both Complex I and Complex II of oxidative phosphorylation have multiple Fe-S clusters and thus require functional ISCU to exist. In addition, iron-sulfur clusters are found in a variety of metalloproteins, such as the ferredoxins, as well as NADH dehydrogenase, hydrogenases, Coenzyme Q - Cytochrome C reductase, Succinate-coenzyme Q reductase and nitrogenase. New research has demonstrated that iron-sulfur proteins also can regulate gene expression and can be regulated by nitric oxide. EMD-Millipore’s Anti-ISCU rabbit polyclonal antibody has been tested in western blots on mouse heart tissue extracts, paraffin embedded immunohistochemistry on human lung cancer sections, and in fluorescent immunocytochemistry on MCF7 cells in culture.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide and 50% glycerol.
      Applications
      ApplicationDetect Iron-sulfur cluster assembly enzyme ISCU using this rabbit polyclonal antibody, Anti-ISCU Antibody validated for use in western blotting, IHC & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ISCU in human lung cancer tissue.

      Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ISCU in MCF-7 cells.
      Biological Information
      ImmunogenGST-tagged fusion protein corresponding to human ISCU.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • ISCU
      • NIFUN
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~18 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse heart tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected ISCU in mouse heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size150 µL
      Transport Information
      Supplemental Information
      Specifications