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MABN483 | Anti-IRAP Antibody, Clone 3E1

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H WB, ICC M Purified Monoclonal Antibody
      Catalogue NumberMABN483
      DescriptionAnti-IRAP Antibody, Clone 3E1
      Alternate Names
      • Leucyl-cystinyl aminopeptidase
      • Cystinyl aminopeptidase
      • Insulin-regulated membrane aminopeptidase
      • Insulin-responsive aminopeptidase
      • IRAP
      • Oxytocinase
      • OTase
      • Placental leucine aminopeptidase
      • P-LAP
      • Leucyl-cystinyl aminopeptidase, pregnancy serum form
      Background InformationIRAP, also known as Leucyl-cystinyl aminopeptidase, Cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase (IRAP), Oxytocinase (OTase), or Placental leucine aminopeptidase (P-LAP) and encoded by the human gene name IRAP/OTASE, is an important aminopeptidase that plays an important role in maintaining homeostasis during pregnancy. IRAP degrades peptide hormones including oxytocin, vasopressin, and angiotensin III; it also appears to play a role in degrading neuronal peptides in the brain such as Met-enkephalin and dynorphin. IRAP also binds angiotensin IV and appears to be the receptor for angiotensin IV in the brain. IRAP is a membrane bound aminopeptidase and is often found within membrane vesicles that together with other proteins like GLUT4 translocates to the plasma membrane in response to insulin or oxytocin. A cleaved fragment of IRAP, the Leucyl-cystinyl aminopeptidase, is found in the blood during pregnancy, low in the first trimester but rising rapidly until birth, whereby it decreases rapidly, thus the secreted form is often used as a pregnancy marker. IRAP is highly expressed in placenta, heart, kidney and small intestine. IRAP is detected at lower levels in neuronal cells in the brain, in skeletal muscle, spleen, liver, testes and colon.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-IRAP, Clone 3E1 is an antibody against IRAP for use in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected IRAP in 3T3-L1 cells treated with or without insulin (Garza, L.A., et al. (2000). JBC. 275(4):2560-2567).
      Immunocytochemistry Analysis: A representative lot detected IRAP in 3T3-L1 cells treated with or without insulin (Garza, L.A., et al. (2000). JBC. 275(4):2560-2567).
      Biological Information
      ImmunogenRecombinant protein corresponding to the N-terminus of human IRAP.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LNPEP
      • OTASE
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~150 kDa observed. The calculated molecular weight of this protein is 117 kDa, but can be observed at ~150 kDa due to high glycosylation. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in insulin treated NIH-3T3 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected IRAP in 10 µg of insulin treated NIH-3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IRAP, Clone 3E1 Q2482135


      Reference overviewPub Med ID
      Insulin-responsive aminopeptidase trafficking in 3T3-L1 adipocytes.
      Garza, LA; Birnbaum, MJ
      The Journal of biological chemistry 275 2560-7 2000

      Show Abstract
      10644714 10644714

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      Life Science Research > Antibodies and Assays > Primary Antibodies