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AB9074 | Anti-IP3 Receptor 2 Antibody

AB9074
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ICC, IHC, WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB9074
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-IP3 Receptor 2 Antibody
      Alternate Names
      • Inositol 1,4,5-Trisphosphate Receptor 2
      References
      Product Information
      FormatSerum
      Control
      • Western blot = adult mouse liver.

        IHC = adult mouse hepatocytes (adult mouse cerebellum Purkinje cells will be negative).
      PresentationRabbit serum. Liquid.
      Applications
      ApplicationDetect IP3 Receptor 2 using this Anti-IP3 Receptor 2 Antibody validated for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:1,000 using ECL. The antibody reacts with the 250 kDa IP3 Receptor 2 protein in microsomal preparations. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel or 5% PAGE. For microsomal methods and blotting procedure similar antibodies can be found in: Perez, et al. J Biol. Chem. 272:23961

      http://www.jbc.org/cgi/reprint/272/38/23961?ijkey=35bcf508a3025fc8bf8203ea3805122eec0caf1e )

      Immunocytochemistry: 1:1,000

      Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from the C terminus of human IP3 Receptor 2.
      HostRabbit
      SpecificityIP3 Receptor 2.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITPR2
      • InsP3R2
      • IP3R2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14571 # Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.
      SIZE: 2701 amino acids; 308078 Da
      SUBUNIT: Homotetramer (By similarity).
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: The short isoform is found in skeletal muscle and heart.
      DOMAIN: SwissProt: Q14571 The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand- binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
      PTM: Phosphorylated on tyrosine residues (By similarity).
      SIMILARITY: Belongs to the InsP3 receptor family. & Contains 5 MIR domains.
      MISCELLANEOUS: Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium- binding protein which then inhibits the receptor.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-IP3 RECEPTOR 2 POLYCLONAL ANTIBODY - 2151813 2151813
      RABBIT ANTI-IP3 RECEPTOR 2 POLYCLONAL ANTIBODY - 2006040 2006040
      RABBIT ANTI-IP3 RECEPTOR 2 POLYCLONAL ANTIBODY - 2031797 2031797
      RABBIT ANTI-IP3 RECEPTOR 2 POLYCLONAL ANTIBODY - 2089410 2089410
      RABBIT ANTI-IP3 RECEPTOR 2 POLYCLONAL ANTIBODY - 2296695 2296695
      RABBIT ANTI-IP3 RECEPTOR 2 POLYCLONAL ANTIBODY - 2344706 2344706

      References

      Reference overviewPub Med ID
      Abolished InsP3R2 function inhibits sweat secretion in both humans and mice.
      Klar, J; Hisatsune, C; Baig, SM; Tariq, M; Johansson, AC; Rasool, M; Malik, NA; Ameur, A; Sugiura, K; Feuk, L; Mikoshiba, K; Dahl, N
      The Journal of clinical investigation 124 4773-80 2014

      Show Abstract
      25329695 25329695
      Endothelial Ca2+ wavelets and the induction of myoendothelial feedback.
      Cam Ha T Tran,Mark S Taylor,Frances Plane,Sridevi Nagaraja,Nikolaos M Tsoukias,Viktoryiya Solodushko,Edward J Vigmond,Tobias Furstenhaupt,Mathew Brigdan,Donald G Welsh
      American journal of physiology. Cell physiology 302 2012

      Show Abstract
      22277756 22277756
      Function and expression of ryanodine receptors and inositol 1,4,5-trisphosphate receptors in smooth muscle cells of murine feed arteries and arterioles.
      Westcott, EB; Goodwin, EL; Segal, SS; Jackson, WF
      The Journal of physiology 590 1849-69 2012

      Show Abstract
      22331418 22331418
      Novel mechanism of increased Ca2+ release following oxidative stress in neuronal cells involves type 2 inositol-1,4,5-trisphosphate receptors.
      Kaja, S; Duncan, RS; Longoria, S; Hilgenberg, JD; Payne, AJ; Desai, NM; Parikh, RA; Burroughs, SL; Gregg, EV; Goad, DL; Koulen, P
      Neuroscience 175 281-91 2011

      Show Abstract
      21075175 21075175
      Progesterone potentiates calcium release through IP3 receptors by an Akt-mediated mechanism in hippocampal neurons.
      Hwang, JY; Duncan, RS; Madry, C; Singh, M; Koulen, P
      Cell calcium 45 233-42 2009

      Show Abstract Full Text Article
      19081133 19081133

      Data Sheet

      Title
      RABBIT ANTI-IP3 RECEPTOR 2

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies