Key Specifications Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H||IP, WB, ICC||M||Purified||Monoclonal Antibody|
|Description||Anti-ING1 Antibody, clone CAb3|
|Presentation||PBS, pH 7.4 and 0.05% sodium azide before the addition of glycerol to 30%|
|Application||Anti-ING1 Antibody, clone CAb3 is a high quality Mouse Monoclonal Antibody for the detection of ING1 & has been validated in IP, WB, ICC.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||2 years at -20°C|
|Material Size||100 µg|
|Anti-ING1, clone CAb3 - 24544||24544|
|Reference overview||Pub Med ID|
|Small molecule-assisted, line-independent maintenance of human pluripotent stem cells in defined conditions. |
Stefan Frank,Miao Zhang,Hans R Schöler,Boris Greber
PloS one 7 2012
Human pluripotent stem cells (hPSCs) are conventionally grown in a mouse feeder cell-dependent manner. Chemically defined culture conditions are, however, desirable not only for potential medically oriented applications but also for investigating mechanisms of self-renewal and differentiation. In light of the rather high complexity and cost of existing defined hPSC culture systems, we have systematically evaluated over 20 potential media ingredients. Only components that reproducibly gave beneficial effects were ultimately combined to yield a simple and cost-effective formulation termed FTDA. This xeno-free medium is based on mimicking self-renewal factor activities present in mouse embryonic fibroblast-conditioned medium, at minimal dosages. Additionally, small molecule inhibitors of BMP and WNT signaling served to specifically suppress typical types of spontaneous differentiation seen in hPSC cultures. FTDA medium was suitable for the generation of human induced pluripotent stem cells and enabled robust long-term maintenance of diverse hPSC lines including hard-to-grow ones. Comparisons with existing defined media suggested reduced spontaneous differentiation rates in FTDA. Our results imply that using supportive factors at minimal concentrations may still promote robust self-renewal and preserve pluripotency of hPSCs.
|Inhibitors of histone deacetylases induce tumor-selective cytotoxicity through modulating Aurora-A kinase. |
Jung-Hyun Park,Hyun-Soon Jong,Sang Gyun Kim,Yeonjoo Jung,Keun-Wook Lee,Ju-Hee Lee,Dae-Kee Kim,Yung-Jue Bang,Tae-You Kim
Journal of molecular medicine (Berlin, Germany) 86 2008
The molecular basis of the antitumor selectivity of histone deacetylase inhibitors (HDIs) remains unclear. Centrosomal Aurora-A kinase regulates chromosomal segregation during mitosis. The overexpression or amplification of Aurora-A leads to genetic instability, and its inhibition has shown significant antitumor effects. In this paper, we report that structurally related hydroxamate LAQ824 and SK-7068 induce tumor-selective mitotic defects by depleting Aurora-A. We found that HDI-treated cancer cells, unlike nontransformed cells, exhibit defective mitotic spindles. After HDI, Aurora-A was selectively downregulated in cancer cells, whereas Aurora-B remained unchanged in both cancer and nontransformed cells. LAQ824 or SK-7068 treatment inhibited histone deacetylase (HDAC) 6 present in Aurora-A/heat shock protein (Hsp) 90 complex. Inhibition of HDAC6 acetylated Hsp90 and resulted in dissociation of acetylated Hsp90 from Aurora-A. As a result, Hsp70 binding to Aurora-A was enhanced in cancer cells, leading to proteasomal degradation of Aurora-A. Overall, these provide a novel molecular basis of tumor selectivity of HDI. LAQ824 and SK-7068 might be more effective HDIs in cancer cells with Aurora-A overexpression.
|A panel of CAb antibodies recognize endogenous and ectopically expressed ING1 protein. |
Boland, D, et al.
Hybridoma, 19: 161-5 (2000) 2000