A part of MilliporeSigma

05-840 | Anti-IDO (Indoleamine 2,3-Dioxygenase) Antibody, clone 10.1

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ICC, WB, IH(P) M Purified Monoclonal Antibody
      Catalogue Number05-840
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IDO (Indoleamine 2,3-Dioxygenase) Antibody, clone 10.1
      Alternate Names
      • Indoleamine 2,3-dioxygenase
      • Indoleamine-pyrrole 2,3-dioxygenase
      • indole 2,3-dioxygenase
      • indoleamine-pyrrole 2,3 dioxygenase
      • IDO1
      Background InformationIndoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
      Product Information
      • Human IFN-gamma stimulated HeLa cell lysates, IFN gamma stimulated human peripheral blood lymphocytes.
      PresentationPurified mouse monoclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
      ApplicationAnti-IDO (Indoleamine 2, 3-Dioxygenase) Antibody, clone 10.1 is an antibody against IDO (Indoleamine 2 for use in IC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunocytochemistry: A previous lot was used by an independent laboratory in Phoenix cells transfected with an expression vector.

      Immunohistochemistry: A previous lot of this antibody was used to detect IDO in human retina, primary eye tumor and metastatic liver tumor tissue sections (Chen, P.W., et al. Experimental Eye Research 85(2007) 617-625).
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 78-184 of human Indoleamine 2,3- Dioxygenase (IDO). Clone 10.1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes IDO (Indoleamine 2,3- Dioxygenase), Mr 42kDa.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]
      Gene Symbol
      • INDO
      • IDO
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14902 # Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
      COFACTOR: Binds 1 heme group per subunit.
      SIZE: 403 amino acids; 45326 Da
      SIMILARITY: SwissProt: P14902 ## Belongs to the indoleamine 2,3-dioxygenase family.
      Molecular Weight42 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on untreated and IFNgamma treated (24 hrs) Hela lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected IDO on 10 ug of untreated and IFN gamma treated Hela lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IDO (Indoleamine 2,3- -2649119 2649119
      Anti-IDO (Indoleamine 2,3- -2760608 2760608
      Anti-IDO (Indoleamine 2,3- Dioxygenase), clone 10.1 - 2290962 2290962
      Anti-IDO (Indoleamine 2,3- Dioxygenase), clone 10.1 - 32352 32352
      Anti-IDO (Indoleamine 2,3- Dioxygenase), clone 10.1 - DAM1695862 DAM1695862
      Anti-IDO (Indoleamine 2,3- Dioxygenase), clone 10.1 - DAM1767478 DAM1767478
      Anti-IDO (Indoleamine 2,3- Dioxygenase), clone 10.1 - DAM1770286 DAM1770286
      Anti-IDO (Indoleamine 2,3- Dioxygenase), clone 10.1 - JBC1938934 JBC1938934
      Anti-IDO (Indoleamine 2,3-Dioxygenase) -2721714 2721714
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 - 2032290 2032290
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 - 2398892 2398892
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 - 2446850 2446850
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 - 2489003 2489003
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 - 2530018 2530018
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 - 27094 27094
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 - DAM1421449 DAM1421449
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 - DAM1810808 DAM1810808
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 -2554901 2554901
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 -2620588 2620588
      Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 -2697431 2697431


      Reference overviewPub Med ID
      Uveal melanoma expression of indoleamine 2,3-deoxygenase: establishment of an immune privileged environment by tryptophan depletion.
      Peter W Chen,Jessamee K Mellon,Elizabeth Mayhew,Shixuan Wang,Yu Guang He,Nick Hogan,Jerry Y Niederkorn
      Experimental eye research 85 2007

      Show Abstract Full Text Article
      17870068 17870068
      Tryptophan and the immune response.
      Moffett, John R and Namboodiri, Ma Aryan
      Immunol. Cell Biol., 81: 247-65 (2003) 2003

      Show Abstract
      12848846 12848846
      Potential regulatory function of human dendritic cells expressing indoleamine 2,3-dioxygenase.
      Munn, David H, et al.
      Science, 297: 1867-70 (2002) 2002

      Show Abstract
      12228717 12228717
      Relationship between interferon-gamma, indoleamine 2,3-dioxygenase, and tryptophan catabolism.
      Taylor, M W and Feng, G S
      FASEB J., 5: 2516-22 (1991) 1991

      Show Abstract
      1907934 1907934
      Mechanism of interferon-gamma action. Characterization of indoleamine 2,3-dioxygenase in cultured human cells induced by interferon-gamma and evaluation of the enzyme-mediated tryptophan degradation in its anticellular activity.
      Takikawa, O, et al.
      J. Biol. Chem., 263: 2041-8 (1988) 1988

      Show Abstract
      3123485 3123485

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      Life Science Research > Antibodies and Assays > Primary Antibodies