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MAB1397Z | Anti-ICAM-1 Antibody, clone 3E2B, Azide Free

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M ELISA, FC, FUNC, ICC, IHC AHm Purified Monoclonal Antibody
      Catalogue NumberMAB1397Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-ICAM-1 Antibody, clone 3E2B, Azide Free
      Alternate Names
      • CD54
      • MAB1397
      Product Information
      PresentationPurified IgG prepared by affinity chromatography on Protein G agarose beads. Liquid at 1 mg/mL in sterile-filtered PBS, containing no preservatives. Endotoxin level 4.13EU/mg
      ApplicationAnti-ICAM-1 Antibody, clone 3E2B, Azide Free detects level of ICAM-1 & has been published & validated for use in ELISA, FC, FUNC, IC, IH.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Affects Function
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry: Live or acetone fixed cells


      Immunohistochemistry of acetone fixed frozen sections. (Paraffin not tested).

      Flow Cytometry: Suggested working dilution 50 μg/mL. Use 5 mL of this dilution to label 0.5 x 10(e6) cells.

      Immunoprecipitation: use protein G or rabbit anti-hamster IgG secondary

      Clone 3E2B is reported to be function blocking against murine ICAM-1.

      Optimal working dilutions must be determined by end user.
      Biological Information
      HostArmenian Hamster
      SpecificityMAB1371Z is reactive with mouse ICAM-1 (CD54), which is present on T-cells, B-cells, monocytes and endothelial cells, epithelial cells, dendritic cells and keratinocytes. Expression of CD54 is increased after stimulation with cytokines and LPS, and it also plays a role in B cell-T cell interactions.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryICAM1 (CD54) is typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. ICAM1 is also exploited by Rhinovirus as a receptor.
      Gene Symbol
      • ICAM1
      • P3.58
      • BB2
      • CD54
      • ICAM-1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05362 # ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). In case of rhinovirus infection acts as a cellular receptor for the virus. Also acts as receptor for some coxsackieviruses.
      SIZE: 532 amino acids; 57825 Da
      SUBUNIT: Interacts with human herpesvirus 8 MIR2 protein (Probable).
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
      SIMILARITY: SwissProt: P05362 ## Belongs to the immunoglobulin superfamily. ICAM family. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number


      Reference overviewPub Med ID
      Protective role of brain water channel AQP4 in murine cerebral malaria.
      Promeneur, D; Lunde, LK; Amiry-Moghaddam, M; Agre, P
      Proceedings of the National Academy of Sciences of the United States of America 110 1035-40 2013

      Show Abstract
      23277579 23277579
      Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1.
      Scheynius, A, et al.
      J. Immunol., 150: 655-63 (1993) 1993

      Show Abstract
      8093459 8093459
      Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55.
      Fabry, Z, et al.
      J. Neuroimmunol., 36: 1-11 (1992) 1992

      Show Abstract
      1346536 1346536
      On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules.
      Johnston, S C, et al.
      J. Immunol., 145: 1181-7 (1990) 1990

      Show Abstract
      2199576 2199576

      Data Sheet

      Anti-ICAM-1, Clone 3E2B, Azide Free - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies