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MABS469 | Anti-IA-2, clone 76F Antibody

MABS469
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IHC, IP M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABS469
      DescriptionAnti-IA-2, clone 76F Antibody
      Alternate Names
      • Receptor-type tyrosine-protein phosphatase-like N
      • R-PTP-N
      • Islet cell antigen 512
      • ICA 512
      • Islet cell autoantigen 3
      • PTP IA-2
      • PTPRN
      Background InformationICA 512, also known as Receptor-type tyrosine-protein phosphatase-like N (R-PTP-N), or Islet cell antigen 512 (ICA 512) or Islet cell autoantigen 3, or PTPIA-2, or IA-2, and encoded by the gene PTPRN/ICA3/ICA512, is a required protein for endocrine and neuroendocrine secretory processes. ICA 512 may be involved in processes specific for neurosecretory granules, such as their biogenesis, trafficking and regulated exocytosis, or ICA 512 may have a more general role in neuroendocrine functions. ICA 512 interacts with phosphorylated syntrophin beta 2/Distrophin, protecting it from protein cleavage by calpain I. Dephosphorylation of Distrophin upon insulin stimulation disrupts the interaction and results in its cleavage. ICA 512 is a membrane protein and found within neuroendocrine secretory granules and endocrine tissues. ICA 512 is expressed in brain, pituitary and gut tissues including the pancreas. ICA 512 is also now used as a diabetes-specific islet autoantigen biomarker in type diabetes patients.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-IA-2 antibody, clone 76F is an antibody against IA-2 for use in western blotting, IHC & IP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A representative lot from an independent laboratory detected IA-2 in rat pancreas tissues and BTC3 cells (Piquer, S., et al. (2006). J Autoimmun. 26(3):215-222.).

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated IA-2 from recombinant rat, mouse, and human IA-2 (Piquer, S., et al. (2006). J Autoimmun. 26(3):215-222.).
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to the residues 626-630 found in juxtamembrane region of human and mouse IA-2, but not IA-2b of human IA-2.
      EpitopeThis antibody recognizes residues 626-630 found in juxtamembrane region of human and mouse IA-2, but not in IA-2b.
      Clone76F
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PTPRN
      • ICA3
      • ICA512
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~65 kDa observed. The full length protein is cleaved to yield a 65 kDa mature protein form that contains a lumenal domain, and an intracellular cytoplasmic domain characterized by a juxtamembrane region (Piquer, S., et al. (2006). J Autoimmun. 26(3):215-222.).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human pancreas tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected IA-2 in 10 µg of human pancreas tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-IA-2, clone 76F - Q2371114 Q2371114

      References

      Reference overviewPub Med ID
      Monoclonal antibody 76F distinguishes IA-2 from IA-2beta and overlaps an autoantibody epitope.
      Piquer, Sandra, et al.
      J. Autoimmun., 26: 215-22 (2006) 2006

      Show Abstract
      16503116 16503116