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ABS1064 | Anti-Hypusine Antibody Antibody

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ICC, IHC Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABS1064
      DescriptionAnti-Hypusine Antibody Antibody
      Background InformationThe amino acid Hypusine is a unique amino acid and a critical component of only one cellular protein, the eukaryotic translation initiation factor 5A (eIF-5A, old terminology eIF-4D). Hypusine is formed post-translationally in two enzymatic steps that modify a specific lysine in the precursor protein of eIF-5A. Hypusine is produced soon after the translation of eIF-5A and the modification is essentially irreversible. Hypusine is found in all eukaryotes and archaebacteria but not in eubacteria, and the high conservation of the amino acids surrounding the key lysine point to its importance in eukaryotic biology. The expression of eIF-5A is essential as is the lysine for eIF-5A function. eIF-5A is needed for the initiation of translation and other important processes such as responses to cellular stress, and nucleocytoplasmic shuttling of specific mRNAs. Without an active Hypusine cell proliferation is inhibited, thus Hypusine and its biosynthesis are targets of anti-cancer therapies.
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 30% glycerol.
      ApplicationThis Anti-Hypusine Antibody Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of Hypusine.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A representative lot of this antibody detected hypusine modification on eIF5A protein in stimulated mouse CD90.2 splenocytes (Colvin, S,. et al, J. Biol. Chem. 2013, 288:36226-36235.)
      Western Blotting Analysis: 0.4 µg/mL of this antibody detected hypusine modifications on eIF5A protein in transfected INS-1, INS-1 β and 293T cell lysate (Nishiki, Y,. et al. SpringerPlus 2013, 2:421)
      Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected hypusine modifications on eIF5A protein in transfected 293T cells (Nishiki, Y,. et al. SpringerPlus 2013, 2:421)
      Immunohistochemistry Analysis: 0.6 µg/mL of this antibody detected hypusine modifications on eIF5A protein in human spleen cells
      Biological Information
      ImmunogenLinear peptide corresponding to Human Hypusine.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityThis antibody detects hypusine modification on endogenous eIF5A
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Purification MethodAntigen Affinity Purified
      Molecular Weight~ 45 kDa observed (Nishiki et al. SpringerPlus 2013, 2:421)
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in transfected HEK293T cell lysate.

      Western Blotting Analysis: 0.6 µg/mL of this antibody detected Hypusine in 10 µg of HEK293T transfected with GFP-eIF5A WT cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Do not store in -80°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Hypusine Antibody Q2473135
      Anti-Hypusine Antibody -2551010 2551010
      Anti-Hypusine Antibody -2578587 2578587
      Anti-Hypusine Antibody -2615069 2615069
      Anti-Hypusine Antibody -2641720 2641720
      Anti-Hypusine Antibody -2710634 2710634
      Anti-Hypusine Antibody -2745595 2745595
      Anti-Hypusine Antibody -2760526 2760526
      Anti-Hypusine Antibody -2805347 2805347
      Anti-Hypusine Antibody -2826069 2826069


      Reference overviewPub Med ID
      Deoxyhypusine synthase promotes differentiation and proliferation of T helper type 1 (Th1) cells in autoimmune diabetes.
      Colvin, SC; Maier, B; Morris, DL; Tersey, SA; Mirmira, RG
      The Journal of biological chemistry 288 36226-35 2013

      Show Abstract
      24196968 24196968
      Characterization of a novel polyclonal anti-hypusine antibody.
      Nishiki, Y; Farb, TB; Friedrich, J; Bokvist, K; Mirmira, RG; Maier, B
      SpringerPlus 2 421 2013

      Show Abstract
      24024105 24024105