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AB5971 | Anti-HuD Antibody

AB5971
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IHC Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5971
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-HuD Antibody
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      Applications
      ApplicationDetect HuD using this Anti-HuD Antibody validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 1:200-1:1,000 using ECL.

      Immunohistochemistry: 1:200-1:1,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide, amino acids 26-42 of HuD.
      HostRabbit
      SpecificityRecognizes HuD. The molecular weight of the protein is 41,766 Daltons.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ELAVL4
      • PNEM
      • HUD
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P26378 # May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity).
      SIZE: 380 amino acids; 41760 Da
      SUBUNIT: Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP (By similarity).
      TISSUE SPECIFICITY: Brain.
      PTM: Methylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth (By similarity).
      DISEASE: SwissProt: P26378 # Paraneoplastic encephalomyelitis sensory neuropathy is associated with small cell lung cancer and is characterized by dementia, sensory loss, and other neurological disabilities. It may result from an immune response primarily directed against a small cell lung tumor antigen which is misdirected against similar antigens expressed in brain.
      SIMILARITY: SwissProt: P26378 ## Belongs to the RRM elav family. & Contains 3 RRM (RNA recognition motif) domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

      PREPARATION AND USE:

      To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

      The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

      For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-HuD - 2492119 2492119
      RABBIT ANTI-HuD -2794780 2794780
      RABBIT ANTI-HuD AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2042750 2042750
      RABBIT ANTI-HuD AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2287691 2287691

      References

      Reference overviewPub Med ID
      Degradation of high affinity HuD targets releases Kv1.1 mRNA from miR-129 repression by mTORC1.
      Sosanya, NM; Huang, PP; Cacheaux, LP; Chen, CJ; Nguyen, K; Perrone-Bizzozero, NI; Raab-Graham, KF
      The Journal of cell biology 202 53-69 2013

      Show Abstract
      23836929 23836929
      Retracted: Overexpression of αCP2, a translational repressor of GAP-43, inhibited axon outgrowth during development in Xenopus laevis.
      Jing Wang,Shenggang Sun,Xuebing Cao,Xuejun Deng,Yunjian Zhang,Qing Zhu
      Biochemical and biophysical research communications 419 2012

      Show Abstract
      22342981 22342981

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

      Data Sheet

      Title
      RABBIT ANTI-HuD AFFINITY PURIFIED POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies