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386040 | Anti-Hsp90 Mouse mAb (AC88)

386040
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      386040-50UG
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          Glass bottle 50 μg
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          Description
          OverviewRecognizes the ~90 kDa Hsp90 protein.
          Catalogue Number386040
          Brand Family Calbiochem®
          SynonymsAnti-Hsp82, Anti-Hsp85, Anti-Hsp89
          Application Data
          Detection of Hsp90 by immunoblotting. Samples: Native human Hsp90 protein (lane 1), recombinant human Hsp90β protein (lane 2), recombinant human Hsp90α protein (lane 3), whole cell lysate from heat shocked HeLa cells (lane 4), heat shocked 3T3 cells (lane 5), heat shocked PC-12 cells (lane 6), heat shocked CHO-K1 cells (lane 7), and heat shocked Rat-2 cells (lane 8). Primary antibody: Anti-Hsp90 Mouse mAb (AC88) (Cat. No. 386040) (1:1000). Detection: chemiluminescence.
          References
          ReferencesHarry, J.L. et al. 1990. Development 190, 305.
          Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937.
          Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
          Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
          Product Information
          FormLiquid
          FormulationIn 10 mM PBS, 50% glycerol, pH 7.2.
          Positive controlHeat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Application NotesImmunoblotting (1 µg/ml, colorimetric)
          Immunoprecipitation (see comments)
          Immunocytochemistry (see comments)
          Application CommentsCross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
          Biological Information
          ImmunogenHsp90 isolated from water mold, Achyla ambisexualis
          ImmunogenAchyla ambisexualis
          CloneAC88
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          386040

          References

          Reference overview
          Harry, J.L. et al. 1990. Development 190, 305.
          Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937.
          Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
          Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-August-2007 RFH
          SynonymsAnti-Hsp82, Anti-Hsp85, Anti-Hsp89
          ApplicationImmunoblotting (1 µg/ml, colorimetric)
          Immunoprecipitation (see comments)
          Immunocytochemistry (see comments)
          Application Data
          Detection of Hsp90 by immunoblotting. Samples: Native human Hsp90 protein (lane 1), recombinant human Hsp90β protein (lane 2), recombinant human Hsp90α protein (lane 3), whole cell lysate from heat shocked HeLa cells (lane 4), heat shocked 3T3 cells (lane 5), heat shocked PC-12 cells (lane 6), heat shocked CHO-K1 cells (lane 7), and heat shocked Rat-2 cells (lane 8). Primary antibody: Anti-Hsp90 Mouse mAb (AC88) (Cat. No. 386040) (1:1000). Detection: chemiluminescence.
          DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
          BackgroundHsp90 (heat shock protein 90) is a primarily cytoplasmic protein which exists in dimeric form. Hsp90 binds several cellular proteins such as retrovirus kinases (pp60v-src), steroid receptors, heme-regulated protein kinase, actin, and tubulin. Although Hsp90 expression is induced by stress, and thus may be an indicator of cell stress, its constitutive level may be up to 1-2% of the total cellular protein.
          HostMouse
          Immunogen speciesAchyla ambisexualis
          ImmunogenHsp90 isolated from water mold, Achyla ambisexualis
          CloneAC88
          IsotypeIgG₁
          Speciesa broad range of species
          Positive controlHeat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
          FormLiquid
          FormulationIn 10 mM PBS, 50% glycerol, pH 7.2.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsCross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesHarry, J.L. et al. 1990. Development 190, 305.
          Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937.
          Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
          Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.