HSP01 | Anti-Hsp72/73 (Ab-1) Mouse mAb (W27)

HSP01
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      HSP01-100UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~72 kDa constitutive and inducible forms of Hsp72/73 in heat-shocked HeLa cells.
          Catalogue NumberHSP01
          Brand Family Calbiochem®
          SynonymsAnti-Heat Shock Protein 72/73
          References
          ReferencesSmith, M.L., et al. 1993. Carcinogenesis 14, 503.
          Lindquist, S. 1986. Ann. Rev. Biochem. 58, 1151.
          Schlesinger, M.J. 1986. J. Cell Biol. 103, 321.
          Ungewickell, E. 1985. EMBO 4, 3385.
          Welch, W.J. and Feramisco, J.R. 1985. Mol. Cell Biol. 5, 1229.
          Velazquez, J.M. and Lindquist, S. 1984. Cell 36, 655.
          Velazquez, J.M., et al. 1983. J. Cell Biol. 96, 286.
          Schlesinger, M.J., et al. 1982. In Heat Shock: From Bacteria to Man. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 440 pgs.
          Kelley, P. and Schlesinger, M.J. 1978. Cell 15, 1277.
          Lemeaux, P.G., et al. 1978. Cell 13, 427.
          Ritossa, F. 1962. Experientia 18, 571.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Negative controlUnshocked HeLa cells
          Positive controlHeat-shocked HeLa cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Paraffin Sections
          Application NotesImmunoblotting (1-5 µg/ml, chemiluminescence)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Paraffin Sections (1-10 µg/ml, no pre-treatment required)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpurified Hsp72/73
          CloneW27
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          HSP01

          References

          Reference overview
          Smith, M.L., et al. 1993. Carcinogenesis 14, 503.
          Lindquist, S. 1986. Ann. Rev. Biochem. 58, 1151.
          Schlesinger, M.J. 1986. J. Cell Biol. 103, 321.
          Ungewickell, E. 1985. EMBO 4, 3385.
          Welch, W.J. and Feramisco, J.R. 1985. Mol. Cell Biol. 5, 1229.
          Velazquez, J.M. and Lindquist, S. 1984. Cell 36, 655.
          Velazquez, J.M., et al. 1983. J. Cell Biol. 96, 286.
          Schlesinger, M.J., et al. 1982. In Heat Shock: From Bacteria to Man. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 440 pgs.
          Kelley, P. and Schlesinger, M.J. 1978. Cell 15, 1277.
          Lemeaux, P.G., et al. 1978. Cell 13, 427.
          Ritossa, F. 1962. Experientia 18, 571.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-August-2007 RFH
          SynonymsAnti-Heat Shock Protein 72/73
          ApplicationImmunoblotting (1-5 µg/ml, chemiluminescence)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Paraffin Sections (1-10 µg/ml, no pre-treatment required)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~72 kDa constitutive and inducible forms of Hsp72/73.
          BackgroundThe heat shock response was first described for Drosophila salivary gland cells, and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial, chicken, and mammalian cells, and were subsequently studied in most organisms. A series of heatshock proteins Hsp 90, Hsp 70, Hsp 20-30 and ubiquitin (8,000 m.w.) are induced by insults such as temperature shock, chemicals and other environmental stress. Hsp 70 levels increase 1000-fold in mammalian and Drosophila cells following induction. This protein concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed. The exact function(s) of the Hsp 70 and other heat shock proteins are not known. Mammalian Hsp 72/73 is reported to be structurally and functionally related to a protein that releases clathrin triskelia from coated vesicles; Hsp 72/73 is known to bind ATP without hydrolyzing it.
          HostMouse
          Immunogenpurified Hsp72/73
          CloneW27
          IsotypeIgG2a
          Specieshuman, mouse, rat
          Positive controlHeat-shocked HeLa cells
          Negative controlUnshocked HeLa cells
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsAntibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesSmith, M.L., et al. 1993. Carcinogenesis 14, 503.
          Lindquist, S. 1986. Ann. Rev. Biochem. 58, 1151.
          Schlesinger, M.J. 1986. J. Cell Biol. 103, 321.
          Ungewickell, E. 1985. EMBO 4, 3385.
          Welch, W.J. and Feramisco, J.R. 1985. Mol. Cell Biol. 5, 1229.
          Velazquez, J.M. and Lindquist, S. 1984. Cell 36, 655.
          Velazquez, J.M., et al. 1983. J. Cell Biol. 96, 286.
          Schlesinger, M.J., et al. 1982. In Heat Shock: From Bacteria to Man. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 440 pgs.
          Kelley, P. and Schlesinger, M.J. 1978. Cell 15, 1277.
          Lemeaux, P.G., et al. 1978. Cell 13, 427.
          Ritossa, F. 1962. Experientia 18, 571.