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386023 | Anti-Hsp47 Mouse mAb (M16.10A1)

386023
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, Ca, Ch, Gp, Ht, H, Mk, M, Porcine, R, Sh M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      386023-50UG
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      Available
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          Glass bottle 50 μg
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          Description
          OverviewRecognizes the ~47 kDa Hsp47 protein.
          Catalogue Number386023
          Brand Family Calbiochem®
          SynonymsAnti-Colligin
          Application Data
          Detection of mouse Hsp47 by immunoblotting. Sample: Whole cell lysate from 3T3 cells. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:1000). Detection: chemiluminescence.

          (left) Detection of rat Hsp47 by staining paraffin sections. Sample: Paraffin-embedded rat carotoid artery after balloon injury to the artery. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023). Detection: colorimetric (DAB). (right) Detection of human Hsp47 by staining paraffin sections. Sample: Human breast cancer tissue. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:50). Detection: colorimetric (DAB).
          References
          ReferencesKuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023.
          Nandan, D., et al. 1998. Exp. Cell Res. 179, 289.
          Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230.
          Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793.
          Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
          Saga, S., et al. 1987. J. Cell Biol. 105, 517.
          Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
          Product Information
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Positive control3T3 cells, Human breast cancer tissue
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Enzyme Immunoassay
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunofluorescence
          Immunoprecipitation
          Paraffin Sections
          Application NotesELISA (see comments)
          Enzyme Immunoassay (see comments)
          Immunoblotting (1 µg/ml, colorimetric)
          Immunocytochemistry (1 µg/ml)
          Immunofluorescence (5 µg/ml)
          Immunoprecipitation (5 µg/ml)
          Paraffin Sections (see comments)
          Application CommentsThis antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.
          Biological Information
          ImmunogenHsp47 from L6 cells
          ImmunogenRat
          CloneM16.10A1
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • Bovine
          • Canine
          • Chicken
          • Guinea Pig
          • Hamster
          • Human
          • Monkey
          • Mouse
          • Porcine
          • Rat
          • Sheep
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          386023

          References

          Reference overview
          Kuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023.
          Nandan, D., et al. 1998. Exp. Cell Res. 179, 289.
          Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230.
          Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793.
          Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
          Saga, S., et al. 1987. J. Cell Biol. 105, 517.
          Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-August-2007 RFH
          SynonymsAnti-Colligin
          ApplicationELISA (see comments)
          Enzyme Immunoassay (see comments)
          Immunoblotting (1 µg/ml, colorimetric)
          Immunocytochemistry (1 µg/ml)
          Immunofluorescence (5 µg/ml)
          Immunoprecipitation (5 µg/ml)
          Paraffin Sections (see comments)
          Application Data
          Detection of mouse Hsp47 by immunoblotting. Sample: Whole cell lysate from 3T3 cells. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:1000). Detection: chemiluminescence.

          (left) Detection of rat Hsp47 by staining paraffin sections. Sample: Paraffin-embedded rat carotoid artery after balloon injury to the artery. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023). Detection: colorimetric (DAB). (right) Detection of human Hsp47 by staining paraffin sections. Sample: Human breast cancer tissue. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:50). Detection: colorimetric (DAB).
          DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.
          BackgroundHsp47 is a unique heat shock protein with collagen binding ability. A member of the serpin family of proteins, Hsp47 localizes within the ER due to an RDEL sequence, a variant of the KDEL sequence. Hsp47 is thought to bind procollagen immediately after it co-translationally enters the ER, and it dissociates from procollagen before its secretion. The ability of Hsp47 to bind collagen I and collagen IV suggests it may function as a molecular chaperone for these proteins.
          HostMouse
          Immunogen speciesRat
          ImmunogenHsp47 from L6 cells
          CloneM16.10A1
          IsotypeIgG2b
          Speciesbovine, canine, chicken, guinea pig, hamster, human, monkey, mouse, porcine, rat, sheep
          Positive control3T3 cells, Human breast cancer tissue
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThis antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesKuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023.
          Nandan, D., et al. 1998. Exp. Cell Res. 179, 289.
          Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230.
          Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793.
          Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
          Saga, S., et al. 1987. J. Cell Biol. 105, 517.
          Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.