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ABE319 | Anti-HpTGEKP motif Antibody

100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC, FC, IP Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABE319
      DescriptionAnti-HpTGEKP motif Antibody
      Alternate Names
      • HpTGEKP
      • global mitotic phosphorylation marker
      Background InformationC2H2 zinc finger proteins (ZFPs) represent the largest family of gene regulators in human. C2H2 ZFPs wrap around DNA via multiple zinc fingers to ensure a stable DNA interaction. Small linker peptides joining adjacent zinc finger modules are critical for the DNA-binding activity of C2H2 ZFPs. The vast majority of linker peptides contain a serine or threonine as their first amino acid residue, phosphorylation of which greatly reduces the DNA binding activity of C2H2 ZFPs. TGEKP is the most common consensus linker sequence found in C2H2 ZFPs, including YY1, Aiolos, TIP20, and Bcl6. Antibody-based detection of H-pTGEKP (H represents the His residue from the preceding zinc finger, and pT represents phospho-Thr) reveals a large scale and tightly synchronized mitotic phosphorylation of ZFPs, starting in mid-prophase right after DNA condensation and before the breakdown of the nuclear envelope. T-lymphokine-activated killer cell-originated protein kinase (TOPK; aka PBK) is activated by Cdk1 and is identified as the kinase responsible for the inactivation of C2H2 ZFPs during mitotic cell division by phosphorylating the TGEKP linker sequence. TOPK/PBK is upregulated in many human cancers and its high expression correlates with poor prognosis.
      Product Information
      FormatAffinity Purified
      • Untreated and Nocodazole treated HeLa acid extracts
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-HpTGEKP motif Antibody is a Rabbit Polyclonal Antibody for detection of HpTGEKP motif also known as HpTGEKP, global mitotic phosphorylation marker & has been validated in WB, ICC, FC & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A representative lot detected TOPK-catalyzed phosphorylation of Aiolos, BCL6, TIP20, and YY1 C2H2 linker sequences in in vitro kinase assays using GST fusion constructs containing the respective linker sequences (Rizkallah, R., et al. (2014). Oncotarget. In press).

      Immunocytochemistry Analysis: A representative lot was used in combination with an anti-pTOPK antibody for a dual immunofluorescent staining of synchronized HeLa cells at different time points after thymidine block release. Spatiotemporal correlation between the mitotic activation of TOPK and C2H2 linker phosphorylation is consistent with TOPK's role as the linker kinase (Rizkallah, R., et al. (2014). Oncotarget. In press).

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot was employed to detect C2H2 linker phosphorylation in HeLa cells following double-thymidine block and release (Rizkallah, R., et al. (2011). Cell Cycle. 10(19):3327-3336).

      Flow Cytometry Analysis: A representative lot was employed to detect C2H2 linker phosphorylation in asynchronous (Asy) and thymidine-arrested (Thy) HeLa cells. (Rizkallah, R., et al. (2011). Cell Cycle. 10(19):3327-3336).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated C2H2 ZFPs with phosphorylated pTGEKP linker sequence from pro-metaphase HeLa cells following nocodazole treatment.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the HpTGEKP motif.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes mulitple phosphorylated mitotic proteins.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Purification MethodAffinity Purfied
      Molecular WeightVarious molecular weights due to presence of conserved motif in multiple proteins.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and nocodazole treated HeLa acid extracts.

      Western Blot Analysis: 1 µg/mL of this antibody detected C2H2 ZFPs with phosphorylated pTGEKP linker sequence in acid extracts from nocodazole-treated, but not untreated HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HpTGEKP motif - 2420615 2420615
      Anti-HpTGEKP motif - 2295484 2295484
      Anti-HpTGEKP motif - Q1966987 Q1966987
      Anti-HpTGEKP motif -2679484 2679484


      Reference overviewPub Med ID
      Identification of non-Ser/Thr-Pro consensus motifs for Cdk1 and their roles in mitotic regulation of C2H2 zinc finger proteins and Ect2.
      Suzuki, K; Sako, K; Akiyama, K; Isoda, M; Senoo, C; Nakajo, N; Sagata, N
      Scientific reports 5 7929 2015

      Show Abstract
      25604483 25604483


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