07-596 Anti-Histone H4 (citrulline 3) Antibody

07-596
200 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ChIP, ICC, IH(P), WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-596
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H4 (citrulline 3) Antibody
      Alternate Names
      • Cit-H4R3
      • H4Cit3
      • Histone H4, Arg3 citrullinated
      Background InformationHistone H4 (UniProt P62805; also known as H4 histone family member A, Histone 1 H4a) is encoded by the HIST1H4A (also known as H4/A, H4FA) gene (Gene ID 8359) in human. Histone H4 is one of the main histone proteins that contribute to the chromatin structure in both prokaryotic and eukaryotic cells. Histone H4 can undergo several different types of epigenetic modifications that influence cellular processes, including methylation and acetylation on lysine and arginine residues, phosphorylation on serine and threonine residues, as well as conversion Arg3 to a citrulline residue by the peptidylarginine deiminase PAD4-catalyzed deimination reaction, also known as citrullination). Histone H4 Arg3 citrullination (H4Cit3) weakens histone-DNA interaction as a result of a conformational change and a reduced positive charge at the histone H4 N terminal end. Bacterial infection is known to stimulate H4Cit3 in neutrophils, forming highly decondensed extracellular chromatin structure NET (neutrophil extracellular trap) that could capture and kill microorganisms. On the other hand, citrullinated proteins are recognized as non-self proteins and subsequently activate immune systems. A functional haplotype of the PADI4 gene has been shown to be associated with the pathogenesis of rheumatoid arthritis through induction of the antigenicity of proteins. H4Cit3 is induced in response to DNA damage through the p53-PADI4 pathway, resulting in upregulated H4Cit3 immunoreactivity localized around fragmented nuclei in apoptotic cells. In addition, H4Cit3 level is negatively correlated with p53 protein expression and tumour size in non-small cell lung cancer (NSCLC) tissues, indicating a crucial role of H4Cit3 in carcinogenesis.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Histone H4 (Citrulline 3) Antibody, Cat. No. 07-596, is a highly specific rabbit polyclonal antibody that targets histone H4 citrullination (H4Cit3) and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesChromatin Immunoprecipitation (ChIP) Analysis: A representative lot (3 µL per ChIP) detected histone H4 Arg3 citrullination (H4Cit3) enrichment at the pS2 gene promoter in MCF-7 cells that had been treated with β-estradiol for one hour after a 3-day serum-starvation period.

      Immunocytochemistry Analysis: A 1:500 dilution detected from a representative lot detected enhanced histone H4 Arg3 citrullination in ionomycin-stimulated primary human neutrophils (Courtesy of Kerri Mowen, Ph.D., Padlock Therapeutics).

      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected enhanced histone H4 Arg3 citrullination (H4Cit3) in 4% paraformaldehyde-fixed, decalcified, paraffin-embedded hind paw sections from wild-type, but not Pad4-knockout mice 5 days post immunization with arthritogenic serum from K/BxN mice (Courtesy of Kerri Mowen, Ph.D., Padlock Therapeutics).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected a time-dependent histone H4 Arg3 citrullination (H4Cit3) enrichment at the pS2 gene promoter in MCF-7 cells following estradiol treatment (Wang, Y., et al. (2004). Science. 306(5694):279-283).

      Immunocytochemistry Analysis: A representative lot detected enhanced nuclear H4/H2A Ar3 citrullination in HEK293T and U2OS cells upon exogenous PAD4 expression or DNA damage induction (Tanikawa, C., et al. (2012). Nat. Commun. 3:676).

      Immunocytochemistry Analysis: A representative lot detected Arg3-citrullinated histone H4 (H4Cit3) in 3.8% paraformaldehyde-fixed, 2% NP-40/1% Triton X-100-permeabilized casein-elicited peritoneal neutrophils cells from wild-type, but not Pad4-knockout mice (Hemmers, S., et al. (2011). PLoS One. 6(7):e22043).

      Immunocytochemistry Analysis: A representative lot detected Arg3-citrullinated histone H4 (H4Cit3) in 3.8% paraformaldehyde-fixed, 2% NP-40/1% Triton X-100-permeabilized infiltrating lung leukocytes isolated from wild-type, but not Pad4-knockout mice following influenza infection (Hemmers, S., et al. (2011). PLoS One. 6(7):e22043).

      Immunocytochemistry Analysis: A representative lot detected Arg3-citrullinated histone H4 (H4Cit3) in 4% paraformaldehyde-fixed, 1% NP-40/0.5% Triton X-100-permeabilized peripheral blood cells from wild-type, but not Pad4-knockout mice 5 days post immunization with arthritogenic serum from K/BxN mice (Rohrbach, A.S., et al. (2012). Arthritis Res. Ther. 14(3):R104).

      Immunocytochemistry Analysis: A representative lot detected Arg3-citrullinated histone H4 (H4Cit3) in synovial cells and extracellular traps (NETs) flushed out of the ankle joints of wild-type, but not Pad4-knockout mice 5 days post immunization with arthritogenic serum from K/BxN mice (Rohrbach, A.S., et al. (2012). Arthritis Res. Ther. 14(3):R104).

      Immunocytochemistry Analysis: A representative lot detected calcium ionophore-induced histone H4 (H4Cit3) Arg3 citrullination in 3.7% paraformaldehyde-fixed, 0.2% NP-40/0.1% Triton X-100-permeabilized HL-60 cells (Wang, Y., et al. (2004). Science. 306(5694):279-283).
      Immunohistochemistry Analysis: A representative lot detected gamma irradiation-induced nuclear H4/H2A Ar3 citrullination in thymus tissue sections from wild-type, but not Pad4-knockout mice (Tanikawa, C., et al. (2012). Nat. Commun. 3:676).

      Immunohistochemistry Analysis: A representative lot detected enhanced histone H4 Arg3 citrullination (H4Cit3) in 4% paraformaldehyde-fixed, decalcified, paraffin-embedded hind paw sections from wild-type, but not Pad4-knockout mice 10 days post immunization with arthritogenic serum from K/BxN mice (Rohrbach, A.S., et al. (2012). Arthritis Res. Ther. 14(3):R104).

      Western Blotting Analysis: A representative lot detected PAD4-catalyzed Arg3 citrullination of recombinant histone H4 and H2A, as well as DNA damage-induced cellular H4 and H2A Ar3 citrullination (Tanikawa, C., et al. (2012). Nat. Commun. 3:676).

      Western Blotting Analysis: A representative lot detected calcium ionophore-induced histone H4 Arg3 citrullination (H4Cit3) in HL-60 cells. Cellular PAD4 knockdown by shRNA treatment greatly diminished ionophor-induced H4Cit3, while antibody blocking with the immunogen peptide prevented target band detection (Wang, Y., et al. (2004). Science. 306(5694):279-283).
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 1-10 (SG[cit]GKGGKGL-C) of human histone H4 (Met1 removed), where [cit] denotes citrullinated Arg3. A C-terminal cysteine was added for conjugation purposes.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detected PAD4-catalyzed Arg3 citrullination of recombinant histone H4 and H2A, as well as calcium ionophore-induced histone H4 Arg3 citrullination (H4Cit3) in HL-60 cells. Cellular PAD4 (peptidyl arginine deiminase 4) knockdown greatly diminished ionophor-induced H4Cit3, while antibody blocking with the immunogen peptide prevented target band detection (Tanikawa, C., et al. (2012). Nat. Commun. 3:676; Wang, Y., et al. (2004). Science. 306(5694):279-283).
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with a broad range of species based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H4A
      • H4/A
      • ;H4FA
      • HIST1H4B
      • H4/I
      • ;H4FI
      • HIST1H4C
      • H4/G
      • H4FG
      • HIST1H4D
      • H4/B
      • H4FB
      • HIST1H4E
      • H4/J
      • H4FJ
      • HIST1H4F
      • H4/C
      • H4FC
      • HIST1H4H
      • H4/H
      • H4FH
      • HIST1H4I
      • H4/M
      • H4FM
      • HIST1H4J
      • H4/E
      • H4FE
      • HIST1H4K
      • H4/D
      • H4FD
      • HIST1H4L
      • H4/K
      • H4FK
      • HIST2H4A
      • H4/N
      • H4F2
      • H4FN
      • HIST2H4
      • HIST2H4B
      • H4/O
      • H4FO
      • HIST4H4
      • HIST1H4A
      • H4/A
      Modifications
      • Citrullination
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~12 kDa observed. 11.24/11.37 kDa (H4 with/without Met1 removal) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HL-60 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected differentiation-induced histone H4 Arg3 citrullination (H4Cit3) in 10 µg of lysate from calcium ionophore-treated HL-60 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Histone H4 (citrulline 3) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone H4 (citrulline 3) Antibody Certificates of Analysis

      TitleLot Number
      Anti-Histone H4 (Citrulline 3)2474906
      Anti-Histone H4 (Citrulline 3) (rabbit immunoaffinity purified IgG) - 21219952121995
      Anti-Histone H4 (Citrulline 3) (rabbit immunoaffinity purified IgG) - 23701382370138
      Anti-Histone H4 (Citrulline 3) (rabbit immunoaffinity purified IgG) - 24204722420472
      Anti-Histone H4 (Citrulline 3) (rabbit immunoaffinity purified IgG) - 24304932430493
      Anti-Histone H4 (Citrulline 3) (rabbit immunoaffinity purified IgG) - 24556082455608
      Anti-Histone H4 (Citrulline 3) (rabbit immunoaffinity purified IgG) - 21931662193166
      Anti-Histone H4 (Citrulline 3) (rabbit immunoaffinity purified IgG) - 22668242266824
      Anti-Histone H4 (Citrulline 3) (rabbit immunoaffinity purified IgG) - 23428772342877
      Anti-Histone H4 (Citrulline 3) - 20731392073139

      References

      Reference overviewApplicationPub Med ID
      Deimination of linker histones links neutrophil extracellular trap release with autoantibodies in systemic autoimmunity.
      Dwivedi, N; Neeli, I; Schall, N; Wan, H; Desiderio, DM; Csernok, E; Thompson, PR; Dali, H; Briand, JP; Muller, S; Radic, M
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  28  2840-51  2014

      Show Abstract
      24671707 24671707
      PAD4-mediated neutrophil extracellular trap formation is not required for immunity against influenza infection.
      Hemmers, S; Teijaro, JR; Arandjelovic, S; Mowen, KA
      PloS one  6  e22043  2011

      Show Abstract Full Text Article
      21779371 21779371
      Potential role for peptidylarginine deiminase 2 (PAD2) in citrullination of canine mammary epithelial cell histones.
      Cherrington, BD; Morency, E; Struble, AM; Coonrod, SA; Wakshlag, JJ
      PloS one  5  e11768  2010

      Show Abstract Full Text Article
      Western Blotting20668670 20668670
      Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
      Shechter, D; Nicklay, JJ; Chitta, RK; Shabanowitz, J; Hunt, DF; Allis, CD
      The Journal of biological chemistry  284  1064-74  2009

      Show Abstract
      18957438 18957438
      Functional connection between deimination and deacetylation of histones.
      Denis, H; Deplus, R; Putmans, P; Yamada, M; Métivier, R; Fuks, F
      Molecular and cellular biology  29  4982-93  2009

      Show Abstract
      19581286 19581286
      Human PAD4 regulates histone arginine methylation levels via demethylimination
      Wang, Y., et al
      Science, 306:279-83 (2004)  2004

      Immunocytochemistry15345777 15345777