05-858 Anti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ch, Vrt, Yeast (S. cerevisiae) WB, Mplex, ChIP-seq, ChIP Rb Purified Monoclonal Antibody
      Catalogue Number05-858
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal
      OverviewThis antibody recognizes histone H4 independant of any post-translational modifications.
      Alternate Names
      • H4
      • Histone H4
      • H4 histone family, member A
      • histone 1
      • H4a
      • histone cluster 1
      • H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      Product Information
      • Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).
      PresentationPurified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide.
      ApplicationAnti-Histone H4 Antibody, pan, clone 62-141-13 is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex, ChIP-seq.
      Key Applications
      • Western Blotting
      • Multiplexing
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesEpitope Mapping (using Luminex®):
      1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).

      Chromatin Immunoprecipitation:
      An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28.
      Epitopea.a. 25-28
      Cloneclone 62-141-13
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes Histone H4, Mr 10 kDa.
      Species Reactivity
      • Human
      • Chicken
      • Vertebrates
      • Yeast (S. cerevisiae)
      Species Reactivity NoteHuman, chicken and yeast. Broad species cross-reactivity expected based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot.

      Western Blot Analysis:
      A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H4, pan - 06110451700611045170
      Anti-Histone H4, pan - 2717127171
      Anti-Histone H4, pan - JBC1361839JBC1361839
      Anti-Histone H4, pan, clone 62-141-132459608
      Anti-Histone H4, pan, clone 62-141-133072362
      Anti-Histone H4, pan, clone 62-141-1305-858
      Anti-Histone H4, pan, clone 62-141-132880725
      Anti-Histone H4, pan, clone 62-141-13 - 21231712123171
      Anti-Histone H4, pan, clone 62-141-13 - 24525212452521
      Anti-Histone H4, pan, clone 62-141-13 - 19572851957285


      Reference overviewApplicationPub Med ID
      Mutation of histone H3 serine 86 disrupts GATA factor Ams2 expression and precise chromosome segregation in fission yeast.
      Lim, KK; Ong, TY; Tan, YR; Yang, EG; Ren, B; Seah, KS; Yang, Z; Tan, TS; Dymock, BW; Chen, ES
      Scientific reports  5  14064  2015

      Show Abstract
      26369364 26369364
      Global regulation of heterochromatin spreading by Leo1.
      Verrier, L; Taglini, F; Barrales, RR; Webb, S; Urano, T; Braun, S; Bayne, EH
      Open biology  5  2015

      Show Abstract
      25972440 25972440
      The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription.
      Creamer, KM; Job, G; Shanker, S; Neale, GA; Lin, YC; Bartholomew, B; Partridge, JF
      Molecular and cellular biology  34  2046-61  2014

      Show Abstract
      Western Blotting24662054 24662054
      ATM increases activation-induced cytidine deaminase activity at downstream S regions during class-switch recombination.
      Khair, L; Guikema, JE; Linehan, EK; Ucher, AJ; Leus, NG; Ogilvie, C; Lou, Z; Schrader, CE; Stavnezer, J
      Journal of immunology (Baltimore, Md. : 1950)  192  4887-96  2014

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      24729610 24729610
      DNA-encoded nucleosome occupancy is associated with transcription levels in the human malaria parasite Plasmodium falciparum.
      Bunnik, EM; Polishko, A; Prudhomme, J; Ponts, N; Gill, SS; Lonardi, S; Le Roch, KG
      BMC genomics  15  347  2014

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      24885191 24885191
      The histone variant composition of centromeres is controlled by the pericentric heterochromatin state during the cell cycle.
      Boyarchuk, E; Filipescu, D; Vassias, I; Cantaloube, S; Almouzni, G
      Journal of cell science  127  3347-59  2014

      Show Abstract
      24906798 24906798
      Promoter decommissioning by the NuRD chromatin remodeling complex triggers synaptic connectivity in the mammalian brain.
      Yamada, T; Yang, Y; Hemberg, M; Yoshida, T; Cho, HY; Murphy, JP; Fioravante, D; Regehr, WG; Gygi, SP; Georgopoulos, K; Bonni, A
      Neuron  83  122-34  2014

      Show Abstract
      24991957 24991957
      High-resolution mapping of chromatin packaging in mouse embryonic stem cells and sperm.
      Carone, BR; Hung, JH; Hainer, SJ; Chou, MT; Carone, DM; Weng, Z; Fazzio, TG; Rando, OJ
      Developmental cell  30  11-22  2014

      Show Abstract
      24998598 24998598
      Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components.
      Alabert, C; Bukowski-Wills, JC; Lee, SB; Kustatscher, G; Nakamura, K; de Lima Alves, F; Menard, P; Mejlvang, J; Rappsilber, J; Groth, A
      Nature cell biology  16  281-93  2014

      Show Abstract
      24561620 24561620
      5-Hydroxymethylcytosine Plays a Critical Role in Glioblastomagenesis by Recruiting the CHTOP-Methylosome Complex.
      Takai, H; Masuda, K; Sato, T; Sakaguchi, Y; Suzuki, T; Suzuki, T; Koyama-Nasu, R; Nasu-Nishimura, Y; Katou, Y; Ogawa, H; Morishita, Y; Kozuka-Hata, H; Oyama, M; Todo, T; Ino, Y; Mukasa, A; Saito, N; Toyoshima, C; Shirahige, K; Akiyama, T
      Cell reports  9  48-60  2014

      Show Abstract
      25284789 25284789