05-858R Anti-Histone H4 Antibody, pan, clone 62-10C-2, rabbit monoclonal

05-858R
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Purified Monoclonal Antibody
      Description
      Catalogue Number05-858R
      DescriptionAnti-Histone H4 Antibody, pan, clone 62-10C-2, rabbit monoclonal
      Alternate Names
      • H4
      • Histone H4
      • H4 histone family, member A
      • histone 1, H4a
      • histone cluster 1, H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      References
      Product Information
      FormatPurified
      Control
      • HeLa acid extract
      PresentationPurified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl), containing 40% glycerol with 0.05% sodium azide
      Applications
      ApplicationUse Anti-Histone H4 Antibody, pan, clone 62-10C-2 (Rabbit Monoclonal Antibody) validated in WB to detect Histone H4 also known as Histone H4.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4.
      EpitopeEpitope mapped to residues 25-28.
      Clone62-10C-2
      HostRabbit
      SpecificityThis antibody recognizes histone H4.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with on human. Broad species cross-reactivity expected based on 100% sequence homology to multiple species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Swiss-Prot P62805# Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.
      Citrullination at Arg-4 by PADI4 impairs methylation.
      Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.
      Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
      Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      Sumoylated, which is associated with transcriptional repression.
      SEQUENCE SIMILARITIES: Belongs to the histone H4 family.
      Molecular Weight 10 kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extracts.

      Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H4 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Histone H4 Antibody, pan, clone 62-10C-2, rabbit monoclonal SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone H4 Antibody, pan, clone 62-10C-2, rabbit monoclonal Certificates of Analysis

      TitleLot Number
      Anti-Histone H4, pan, clone 62-10C-2 - 19672861967286
      Anti-Histone H4, pan, clone 62-10C-2 - 20909502090950
      Anti-Histone H4, pan, clone 62-10C-2 - 22072022207202
      Anti-Histone H4, pan, clone 62-10C-2 - 23220122322012
      Anti-Histone H4, pan, clone 62-10C-2 - 32197273219727
      Anti-Histone H4, pan, clone 62-10C-2 - JBC1771287JBC1771287
      Anti-Histone H4, pan, clone 62-10C-2 - NG1865066NG1865066
      Anti-Histone H4, pan, clone 62-10C-2 - NRG1691089NRG1691089
      Anti-Histone H4, pan, clone 62-10C-2 -25089172508917
      Anti-Histone H4, pan, clone 62-10C-2 -27616282761628

      References

      Reference overviewApplicationPub Med ID
      Histone deacetylase (HDAC) inhibitor kinetic rate constants correlate with cellular histone acetylation but not transcription and cell viability.
      Lauffer, BE; Mintzer, R; Fong, R; Mukund, S; Tam, C; Zilberleyb, I; Flicke, B; Ritscher, A; Fedorowicz, G; Vallero, R; Ortwine, DF; Gunzner, J; Modrusan, Z; Neumann, L; Koth, CM; Lupardus, PJ; Kaminker, JS; Heise, CE; Steiner, P
      The Journal of biological chemistry  288  26926-43  2013

      Show Abstract
      Western Blotting23897821 23897821