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382160 | Anti-Histone H4, Acetylated (2-19) Rabbit pAb

382160
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, T Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      382160-50UG
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          Glass bottle 50 μg
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          Description
          OverviewRecognizes the ~10 kDa acetylated histone H4 protein in Tetrahymena and sodium butyrate-treated HeLa cells.
          Catalogue Number382160
          Brand Family Calbiochem®
          Application Data
          Detection of human acetylated histone H4 by immunoblotting. Samples: Acid-extracted proteins from HeLa cells treated with sodium butyrate at antibody concentrations of 0.5 µg/ml (lane 1) and 2 µg/ml (lane 2). Primary antibody: Anti-Histone H4, Acetylated (2-19) Rabbit pAb (Cat. No. 382160) (2 µg/ml). Detection: chemiluminescence.
          References
          ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
          Braunstein, M., et al. 1993. Genes Devel. 7, 592.
          Perry, C.A., et al. 1993. Biochemistry 32, 13605.
          Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
          Positive controlTetrahymena and sodium butyrate-treated HeLa cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Application NotesImmunoblotting (0.5-2 µg/ml)
          Immunocytochemistry (5-10 µg/ml)
          Immunoprecipitation (5µg/500 µg lysate)
          Application CommentsThe antibody detected acetylated histone H4 in acid-extracted proteins from HeLa cells. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with histone H2B from HeLa cells. May cross-react with other acetylated proteins. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H4. For immunocytochemistry, human A431 cells were treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h and fixed with ice-cold 1% paraformaldehyde in PBS. Variables associated with assay conditions will dictate the optimal dilution.
          Biological Information
          Immunogena tetra-acetylated synthetic peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS(C)] corresponding to amino acids 2-19 of Tetrahymena histone H4, conjugated to KLH
          ImmunogenTetrahymena
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Tetrahymena
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          382160

          References

          Reference overview
          Braunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
          Braunstein, M., et al. 1993. Genes Devel. 7, 592.
          Perry, C.A., et al. 1993. Biochemistry 32, 13605.
          Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-August-2007 RFH
          ApplicationImmunoblotting (0.5-2 µg/ml)
          Immunocytochemistry (5-10 µg/ml)
          Immunoprecipitation (5µg/500 µg lysate)
          Application Data
          Detection of human acetylated histone H4 by immunoblotting. Samples: Acid-extracted proteins from HeLa cells treated with sodium butyrate at antibody concentrations of 0.5 µg/ml (lane 1) and 2 µg/ml (lane 2). Primary antibody: Anti-Histone H4, Acetylated (2-19) Rabbit pAb (Cat. No. 382160) (2 µg/ml). Detection: chemiluminescence.
          DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~10 kDa acetylated histone H4 protein.
          BackgroundThe acetylation of core histones regulates gene expression through alterations in the chromatin structure. Lysine acetylation in the amino-terminal domains of histones H3 and H4 can alter the topology of the DNA in chromatin and probably influence its higher-order folding.
          HostRabbit
          Immunogen speciesTetrahymena
          Immunogena tetra-acetylated synthetic peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS(C)] corresponding to amino acids 2-19 of Tetrahymena histone H4, conjugated to KLH
          IsotypeIgG
          SpeciesTetrahymena, human
          Positive controlTetrahymena and sodium butyrate-treated HeLa cells
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThe antibody detected acetylated histone H4 in acid-extracted proteins from HeLa cells. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with histone H2B from HeLa cells. May cross-react with other acetylated proteins. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H4. For immunocytochemistry, human A431 cells were treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h and fixed with ice-cold 1% paraformaldehyde in PBS. Variables associated with assay conditions will dictate the optimal dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
          Braunstein, M., et al. 1993. Genes Devel. 7, 592.
          Perry, C.A., et al. 1993. Biochemistry 32, 13605.
          Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.