ABE1791-25UL Anti-Histone H3Q5ser

NewABE1791-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE1791-25UL
      DescriptionAnti-Histone H3Q5ser
      Alternate Names
      • H3Q5Ser
      Background InformationHistone H3.1 UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histone 3.1 can also undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. This H3Q5ser antibody only detects signal when the nucleosomes have been transamidated with serotonin and does not react with unmodified version.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Histone H3Q5ser, Cat. No. ABE1791, is a highly specific rabbit polyclonal antibody that targets Histone H3.1 serotonylated at glutamine 5 position and has been tested for use in ChIP-seq, Dot Blot, and Western Blotting.
      Key Applications
      • ChIP-seq
      • Dot Blot
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representaive lot detected Histone H3Q5ser in Western Blotting applications (Farrelly, L.A., et. al. (2019). Nature. 567(7749):535-539).

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected Histone H3Q5ser in acid extracted histones from whole mouse brain (Courtesy of Dr. Ian Maze, Icahn School of Medicine at Mount Sinai, New York).

      Dot Blot Analysis: Unmodified and various modified Histone peptides were probed with Histone H3Q5ser (Courtesy of Dr. Ian Maze, Icahn School of Medicine at Mount Sinai, New York).

      ChIP-seq Analysis: A representative lot detected Histone H3Q5ser in DNA input (differentiated RN46A-B14 cells) (Courtesy of Dr. Ian Maze, Icahn School of Medicine at Mount Sinai, New York).


      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3.1 serotonylated on glutamine 5.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Histone H3.1 serotonylated at glutamine 5 position.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~16 kDa observed; 15.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot on H3Q5-Serotonyl peptide.



      Dot Blot Analysis: A 1:500 dilution of this antibody detected Histone H3Q5serH3Q5-Serotonyl peptide, but not the unmodified H3(1-10)-control peptide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Histone H3Q5ser Certificates of Analysis

      TitleLot Number
      Anti-Histone H3Q5ser - 32693443269344

      References

      Reference overviewPub Med ID
      Histone serotonylation is a permissive modification that enhances TFIID binding to H3K4me3.
      Farrelly, LA; Thompson, RE; Zhao, S; Lepack, AE; Lyu, Y; Bhanu, NV; Zhang, B; Loh, YE; Ramakrishnan, A; Vadodaria, KC; Heard, KJ; Erikson, G; Nakadai, T; Bastle, RM; Lukasak, BJ; Zebroski, H; Alenina, N; Bader, M; Berton, O; Roeder, RG; Molina, H; Gage, FH; Shen, L; Garcia, BA; Li, H; Muir, TW; Maze, I
      Nature  567  535-539  2019

      Show Abstract
      30867594 30867594