ABE2580-25UL Anti-Histone H3Kme3Q5Ser

NewABE2580-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE2580-25UL
      DescriptionAnti-Histone H3Kme3Q5Ser
      Alternate Names
      • H3KmeQ5Ser
      Background InformationHistone H3.1 UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histone 3.1 can also undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. This has been demonstrated that tissue transglutaminase 2 can serotonylate histone H3K4me3 marked nucleosomes, which results in the formation of H3K4me3Q5ser. The presence of Q5ser does not affect the ability of MLL1, an H3K4 methyltransferase, to modify nucleosomes in vitro. In the brain the H3K4me3Q5ser signal is not segregated only to regions in which serotonin is produced, but is shown to be broadly distributed. H3K4me3Q5ser expression has also been reported in human pluripotent stem cell-derived serotonergic neurons and with differentiation the levels of H3K4me3Q5ser increase significantly.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Histone H3Kme3Q5Ser, Cat. No. ABE2580, is a highly specific rabbit polyclonal antibody that targets Histone H3 trimethylated on Lysine 4 and serotonylated on glutamine 5 and has been tested for use in ChIP-seq, Dot Blot, and Western Blotting.
      Key Applications
      • ChIP-seq
      • Dot Blot
      • Western Blotting
      Application NotesChIP-seq Analysis: A representaive lot detected Histone H3Kme3Q5Ser in ChIP-seq applications (Farrelly, L.A., et. al. (2019). Nature. 567(7749):535-539).

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected Histone H3.1t in whole cell lysates from mouse brain across multiple regions (hippocampus, prefrontal cortex, ventral tegmental area, dorsal raphe nucleus, nucleus accumbens, caudate putamen, cerebellum). (Courtesy of Dr. Ian Maze, Icahn School of Medicine at Mount Sinai, New York).

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected Histone H3Kme3Q5Ser in whole cell lysate from Undifferentoated & differentiated RN46a-B14 cells.

      Western Blotting Analysis: A representaive lot detected Histone H3Kme3Q5Ser in Western Blotting applications (Farrelly, L.A., et. al. (2019). Nature. 567(7749):535-539).

      ChIP-seq Analysis: A representative lot detected Histone H3Kme3Q5Ser in H3K4me3Q5ser at Fos (brain expressed) vs. Hpx (liver specific gene) loci showing nice enrichment in brain over DNA input (mouse dorsal raphe nucleus, adult). (Courtesy of Dr. Ian Maze, Icahn School of Medicine at Mount Sinai, New York).

      Dot Blot Analysis: Unmodified and various modified Histone peptides were probed with H3Kme3Q5Ser (Courtesy of Dr. Ian Maze, Icahn School of Medicine at Mount Sinai, New York).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3 trimethylated on lysine 4 and serotonylated on glutamine 5.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Histone H3 trimethylated on Lysine 4 and serotonylated on glutamine 5.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~16 kDa observed; 15.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot on H3(1-10)-control peptide and H3K4me3Q5serotonyl peptide.



      Dot Blot Analysis: A 1:500 dilution of this antibody detected Histone H3Kme3Q5Ser peptide, but not the control in H3(1-10) peptide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Histone H3Kme3Q5Ser Certificates of Analysis

      TitleLot Number
      Anti-Histone H3Kme3Q5Ser - 32693463269346

      References

      Reference overviewPub Med ID
      Histone serotonylation is a permissive modification that enhances TFIID binding to H3K4me3.
      Farrelly, LA; Thompson, RE; Zhao, S; Lepack, AE; Lyu, Y; Bhanu, NV; Zhang, B; Loh, YE; Ramakrishnan, A; Vadodaria, KC; Heard, KJ; Erikson, G; Nakadai, T; Bastle, RM; Lukasak, BJ; Zebroski, H; Alenina, N; Bader, M; Berton, O; Roeder, RG; Molina, H; Gage, FH; Shen, L; Garcia, BA; Li, H; Muir, TW; Maze, I
      Nature  567  535-539  2019

      Show Abstract
      30867594 30867594