ABE154 Anti-Histone H3.1/H3.2 Antibody

ABE154
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H WB, ChIP-seq, ChIP Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABE154
      DescriptionAnti-Histone H3.1/H3.2 Antibody
      Alternate Names
      • Histone H3.1
      • Histone H3/a
      • Histone H3/b
      • Histone H3/c
      • Histone H3/d
      • Histone H3/f
      • Histone H3/h
      • Histone H3/I
      • Histone H3/j
      • Histone H3/k
      • Histone H3/l
      Background InformationHistone H3.1 and H3.2 are closely related histone variants that differ by only one amino acid. Mammalian cells express three major types of noncentromeric histone H3 variants, H3.1, H3.2, and H3.3. H3.1 is only expressed in mammals, while H3.2 is found in all eukaryotes except for yeast. Recent evidence shows that these nearly identical histone proteins differ in their expression profiles and post-translational modifications (PTMs). H3.2 is enriched in modifications associated with transcriptional silencing (di- and tri-methylation of K27), whereas H3.1 is enriched in both K9 dimethylation and K14 acetylation, suggesting that these variants may have separate biological functions.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa acid extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Histone H3.1/H3.2 Antibody is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting & ChIP-seq.
      Key Applications
      • Western Blotting
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: A representative lot detected Histone H3.2 in recombinant Histone H3.2 and mouse Embryonic Stem cell lysates.

      Chromatin Immunprecipitation: A representative lot detected Histone H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University).

      Chromatin Immunoprecipitation Sequencing: A representative lot detected Histone H3.1/H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Histone H3.1 and H3.2.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody is expected to demonstrate broad species reactivity.
      Species Reactivity
      • Mouse
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized bands may be observed at ~25 kDa and ~50 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

      Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Histone H3.1 and H3.2 in 10 μg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Histone H3.1/H3.2 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone H3.1/H3.2 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Histone H3.1/H3.2 - Q2147394Q2147394
      Anti-Histone H3.1/H3.2 - 31874033187403
      Anti-Histone H3.1/H3.2 -25238832523883
      Anti-Histone H3.1/H3.2 -25500682550068
      Anti-Histone H3.1/H3.2 -25729982572998
      Anti-Histone H3.1/H3.2 -26436162643616
      Anti-Histone H3.1/H3.2 -26731732673173
      Anti-Histone H3.1/H3.2 -26794822679482
      Anti-Histone H3.1/H3.2 -26877932687793
      Anti-Histone H3.1/H3.2 -27106242710624

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)