05-1341 Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301

05-1341
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt WB, ICC, ELISA, IP, FC, DB M Affinity Purified Monoclonal Antibody
      Description
      Catalogue Number05-1341
      DescriptionAnti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301
      Alternate Names
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa Acid extract lysate.
      PresentationMouse monoclonal IgG2bκ supernatant in PBS with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, (Mouse Monoclonal Antibody) validated in WB, ICC, ELISA, IP, FC, DB to detect Histone H3 also known as histone 3-H3.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • ELISA
      • Immunoprecipitation
      • Flow Cytometry
      • Dot Blot
      Application NotesELISA:
      This antibody has been shown by an outside laboratory to be suitable for ELISA.1

      Immunocytochemistry:
      This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1

      Immunoprecipitation:
      This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 1-12 of human Histone H3, conjugated to KLH.
      EpitopeN-terminus (unmodified)
      CloneCMA301
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Histone H3 that is unmodified at Thr3 and Lys4 but does not recognize phosphorylated Thr3 nor acetylated or methylated Lys4. The antibody binding specificity allows modifications of Lys9, Ser10, and/or Lys141.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Vertebrates
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected, based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is
      further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • H3.4
      • H3/g
      • H3/t
      • H3FT
      • H3T
      • H3t
      • MGC126886
      • MGC126888
      • OTTHUMP00000037945
      Purification MethodPeptide affinity chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Expressed in testicular cells.

      Developmental stage Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 By similarity.

      Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription By similarity.

      Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression By similarity.

      Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin By similarity.

      Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation By similarity.

      Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

      Ubiquitinated By similarity.

      SIMILARITY:Belongs to the histone H3 family.

      Molecular WeightApprox. 17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa cell lysate.

      Western Blot Analysis: 1:10,000 dilution of this antibody detected histone H3 in 10 μg of HeLa Acid Extract cell lysate and recombinant histone H3 (500 ng) but not other recombinant histones.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301 Certificates of Analysis

      TitleLot Number
      Anti-Histone H3 (Unmodified Lys4), -28171862817186
      Anti-Histone H3 (Unmodified Lys4), clone CMA301 - 23903552390355
      Anti-Histone H3 (Unmodified Lys4), clone CMA301 - 24531792453179
      Anti-Histone H3 (Unmodified Lys4), clone CMA301 - 22727112272711
      Anti-Histone H3 (Unmodified Lys4), clone CMA301 - 25004012500401
      Anti-Histone H3 (Unmodified Lys4), clone CMA301 - 31821263182126
      Anti-Histone H3 (Unmodified Lys4), clone CMA301 - 32199303219930
      Anti-Histone H3 (Unmodified Lys4), clone CMA301 -26104952610495
      Anti-Histone H3 (Unmodified Lys4), clone CMA301 -27283812728381
      Anti-Histone H3 (Unmodified Lys4), clone CMA301 -27727432772743

      References

      Reference overviewApplicationPub Med ID
      Embryonic MicroRNA-369 Controls Metabolic Splicing Factors and Urges Cellular Reprograming.
      Konno, M; Koseki, J; Kawamoto, K; Nishida, N; Matsui, H; Dewi, DL; Ozaki, M; Noguchi, Y; Mimori, K; Gotoh, N; Tanuma, N; Shima, H; Doki, Y; Mori, M; Ishii, H
      PloS one  10  e0132789  2015

      Show Abstract
      26176628 26176628
      Radiation-induced alterations of histone post-translational modification levels in lymphoblastoid cell lines.
      Maroschik, B; Gürtler, A; Krämer, A; Rößler, U; Gomolka, M; Hornhardt, S; Mörtl, S; Friedl, AA
      Radiation oncology (London, England)  9  15  2014

      Show Abstract
      Western Blotting24406105 24406105
      ADAM17-mediated CD44 cleavage promotes orasphere formation or stemness and tumorigenesis in HNSCC.
      Kamarajan, P; Shin, JM; Qian, X; Matte, B; Zhu, JY; Kapila, YL
      Cancer medicine  2  793-802  2013

      Show Abstract
      24403253 24403253
      The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies.
      Kimura, Hiroshi, et al.
      Cell Struct. Funct., 33: 61-73 (2008)  2008

      18227620 18227620
      Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena.
      Wei, Y, et al.
      Proc. Natl. Acad. Sci. U.S.A., 95: 7480-4 (1998)  1998

      9636175 9636175
      Alteration of cell cycle-dependent histone phosphorylations by okadaic acid. Induction of mitosis-specific H3 phosphorylation and chromatin condensation in mammalian interphase cells.
      Ajiro, K, et al.
      J. Biol. Chem., 271: 13197-201 (1996)  1996

      8662672 8662672
      Increased phosphorylation of histone H1 in mouse fibroblasts transformed with oncogenes or constitutively active mitogen-activated protein kinase kinase.
      Chadee, D N, et al.
      J. Biol. Chem., 270: 20098-105 (1995)  1995

      Show Abstract
      7650028 7650028

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      Life Science Research > Antibodies and Assays > Primary Antibodies