07-690 Anti-Histone H3 Antibody, CT, pan

07-690
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, M, R, Yeast (S. cerevisiae) WB, ChIP Rb Serum Polyclonal Antibody
      Description
      Catalogue Number07-690
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H3 Antibody, CT, pan
      Alternate Names
      • H3
      • Histone H3
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatSerum
      Control
      • HeLa cell extract
      PresentationRabbit antiserum diluted 1:2 in storage buffer (0.02 M Phosphate, 0.25 M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%.
      Applications
      ApplicationUse Anti-Histone H3 Antibody, CT, pan (rabbit polyclonal antibody) validated in ChIP, WB to detect Histone H3 also known as H3, Histone H3, H3 histone family member T, histone 3 H3, histone cluster 3 H3.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes C-Terminal region of Histone H3, Mr 17 kDa
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Rat
      • Yeast (S. cerevisiae)
      Species Reactivity NoteHuman, mouse, rat, yeast and chicken. Broad species cross-reactivity expected due to sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      • H3/t
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular WeightApprox. 17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

      Western Blot Analysis:
      1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Histone H3 Antibody, CT, pan SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone H3 Antibody, CT, pan Certificates of Analysis

      TitleLot Number
      Anti-Histone H3, CT, pan3068449
      Anti-Histone H3, CT, pan - 21160182116018
      Anti-Histone H3, CT, pan - 21541872154187
      Anti-Histone H3, CT, pan - 24246722424672
      Anti-Histone H3, CT, pan - 07020524440702052444
      Anti-Histone H3, CT, pan - 20197432019743
      Anti-Histone H3, CT, pan - 20737842073784
      Anti-Histone H3, CT, pan - 22434992243499
      Anti-Histone H3, CT, pan - 22966982296698
      Anti-Histone H3, CT, pan - 3037430374

      References

      Reference overviewApplicationSpeciesPub Med ID
      Spatiotemporal cascade of transcription factor binding required for promoter activation.
      Yarrington, RM; Rudd, JS; Stillman, DJ
      Molecular and cellular biology  35  688-98  2015

      Show Abstract
      25512608 25512608
      Parallel action of AtDRB2 and RdDM in the control of transposable element expression.
      Clavel, M; Pélissier, T; Descombin, J; Jean, V; Picart, C; Charbonel, C; Saez-Vásquez, J; Bousquet-Antonelli, C; Deragon, JM
      BMC plant biology  15  70  2015

      Show Abstract
      25849103 25849103
      An integrative analysis of post-translational histone modifications in the marine diatom Phaeodactylum tricornutum.
      Veluchamy, A; Rastogi, A; Lin, X; Lombard, B; Murik, O; Thomas, Y; Dingli, F; Rivarola, M; Ott, S; Liu, X; Sun, Y; Rabinowicz, PD; McCarthy, J; Allen, AE; Loew, D; Bowler, C; Tirichine, L
      Genome biology  16  102  2015

      Show Abstract
      25990474 25990474
      Mutant IDH1 Dysregulates the Differentiation of Mesenchymal Stem Cells in Association with Gene-Specific Histone Modifications to Cartilage- and Bone-Related Genes.
      Jin, Y; Elalaf, H; Watanabe, M; Tamaki, S; Hineno, S; Matsunaga, K; Woltjen, K; Kobayashi, Y; Nagata, S; Ikeya, M; Kato, T; Okamoto, T; Matsuda, S; Toguchida, J
      PloS one  10  e0131998  2015

      Show Abstract
      Western Blotting26161668 26161668
      Analysis of Histones H3 and H4 Reveals Novel and Conserved Post-Translational Modifications in Sugarcane.
      Moraes, I; Yuan, ZF; Liu, S; Souza, GM; Garcia, BA; Casas-Mollano, JA
      PloS one  10  e0134586  2015

      Show Abstract
      26226299 26226299
      Rationale for poly(ADP-ribose) polymerase (PARP) inhibitors in combination therapy with camptothecins or temozolomide based on PARP trapping versus catalytic inhibition.
      Murai, J; Zhang, Y; Morris, J; Ji, J; Takeda, S; Doroshow, JH; Pommier, Y
      The Journal of pharmacology and experimental therapeutics  349  408-16  2014

      Show Abstract
      24650937 24650937
      Low-dose formaldehyde delays DNA damage recognition and DNA excision repair in human cells.
      Luch, A; Frey, FC; Meier, R; Fei, J; Naegeli, H
      PloS one  9  e94149  2014

      Show Abstract
      Western BlottingHuman24722772 24722772
      Nuclear AXIN2 represses MYC gene expression.
      Rennoll, SA; Konsavage, WM; Yochum, GS
      Biochemical and biophysical research communications  443  217-22  2014

      Show Abstract
      Western Blotting24299953 24299953
      Hypoxic preconditioning differentially affects GABAergic and glutamatergic neuronal cells in the injured cerebellum of the neonatal rat.
      Benitez, SG; Castro, AE; Patterson, SI; Muñoz, EM; Seltzer, AM
      PloS one  9  e102056  2014

      Show Abstract
      Western BlottingRat25032984 25032984
      Poly(ADP-ribosyl)ation is required to modulate chromatin changes at c-MYC promoter during emergence from quiescence.
      Mostocotto, C; Carbone, M; Battistelli, C; Ciotti, A; Amati, P; Maione, R
      PloS one  9  e102575  2014

      Show Abstract
      25047032 25047032

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      Life Science Research > Antibodies and Assays > Primary Antibodies