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07-718 | Anti-Histone H2A.Z Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Yeast WB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-718
      Trade Name
      • Upstate
      DescriptionAnti-Histone H2A.Z Antibody
      Alternate Names
      • H2AZ
      • HTA3
      • H2AZ
      • Htz1
      Background InformationH2A.Z, also known as H2AFZ, is a variant of histone H2A variant that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
      Product Information
      FormatAffinity Purified
      • Yeast whole cell extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Histone H2A.Z Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A.Z also known as H2AZ histone & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of yeast Histone H2A.Z.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the C-terminus of Histone H2A.Z.
      Species Reactivity
      • Yeast
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HTZ1
      • H2AZ
      • HTA3
      • OL012C
      • O2345
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Variant histone H2A which can replace H2A in some nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. This variant is enriched at promoters, it may keep them in a repressed state until the appropriate activation signal is received. Near telomeres, it may counteract gene silencing caused by the spread of heterochromatin proteins. Required for the RNA polymerase II and SPT15/TBP recruitment to the target genes. Involved in chromosome stability.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2A.Z forms an heterodimer with H2B. H2A.Z associates with the VPS72/SWC2 subunit of the SWR1 chromatin remodeling complex. Interacts also with RBP1/DNA-directed RNA polymerase II largest subunit. Interacts with NAP1.

      SUBCELLULAR LOCATION: Nucleus. Chromosome.
      Molecular Weight~16 kDa observed. Uncharacterized bands may be observed at ~37 kDa and ~44 kDa in some cell lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in yeast whole cell extract.

      Western Blot Analysis: 0.05 µg/mL of this antibody detected Histone H2A.Z in 6 µL of yeast whole cell extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H2A.Z - 2395707 2395707
      Anti-Histone H2A.Z - 2450105 2450105
      Anti-Histone H2A.Z - 1961032 1961032
      Anti-Histone H2A.Z - 2365792 2365792
      Anti-Histone H2A.Z - 2531466 2531466
      Anti-Htz1 (rabbit immunoaffinity purified IgG) - DAM1421454 DAM1421454
      Anti-Htz1 - 30459 30459
      Anti-Htz1 - DAM1528025 DAM1528025


      Reference overviewApplicationPub Med ID
      The INO80 chromatin remodeling complex prevents polyploidy and maintains normal chromatin structure at centromeres.
      Chambers, AL; Ormerod, G; Durley, SC; Sing, TL; Brown, GW; Kent, NA; Downs, JA
      Genes & development 26 2590-603 2012

      Show Abstract
      Western Blotting23207916 23207916
      Conserved histone variant H2A.Z protects euchromatin from the ectopic spread of silent heterochromatin.
      Meneghini, Marc D, et al.
      Cell, 112: 725-36 (2003) 2003

      Show Abstract
      12628191 12628191
      A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1.
      Krogan, Nevan J, et al.
      Mol. Cell, 12: 1565-76 (2003) 2003

      Show Abstract
      14690608 14690608
      H2A.Z is required for global chromatin integrity and for recruitment of RNA polymerase II under specific conditions.
      Adam, M, et al.
      Mol. Cell. Biol., 21: 6270-9 (2001) 2001

      Show Abstract
      11509669 11509669
      Histone H2A.Z has a conserved function that is distinct from that of the major H2A sequence variants.
      Jackson, J D and Gorovsky, M A
      Nucleic Acids Res., 28: 3811-6 (2000) 2000

      Show Abstract
      11000274 11000274

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      Life Science Research > Antibodies and Assays > Primary Antibodies