07-146 Anti-Histone H2A (acidic patch) Antibody

07-146
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, Ch, Yeast (S. cerevisiae) WB, ChIP Rb Serum Polyclonal Antibody
      Description
      Catalogue Number07-146
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H2A (acidic patch) Antibody
      Alternate Names
      • H2A histone family, member Q
      • histone 2
      • H2ac
      • histone IIa
      • histone cluster 2
      • H2ac
      Background InformationHistone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      References
      Product Information
      FormatSerum
      Control
      • HeLa cell extract.
      PresentationRabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationAnti-Histone H2A (acidic patch) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A (acidic patch) also known as histone 2-H2ac, histone IIa & has been validated in ChIP & WB.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunofluorescence:
      Recommend Anti-phospho-Histone H2A.X (Ser139), clone JBW301, catalog #05-636.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 88-97 (IRNDEELNKL-C) of human Histone H2A.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Histone H2A, Mr 14 kDa.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Chicken
      • Yeast (S. cerevisiae)
      Species Reactivity NoteHuman, mouse, rat, yeast and chicken. Broad species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family.
      Gene Symbol
      • HIST2H2AC
      • H2A-GL101
      • H2AFQ
      • H2A/r
      • H2A/q
      • MGC74460
      • H2A
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16777 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 129 amino acids; 13988 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: The chromatin-associated form is phosphorylated on Thr-121 during mitosis (Probable). & Deiminated on Arg-4 in granulocytes upon calcium entry. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. & Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1. & Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity).
      SIMILARITY: SwissProt: Q16777 ## Belongs to the histone H2A family.
      Molecular Weight14 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on acid extracts from HeLa cell lysate.

      Western Blot Analysis:
      1:1000-1:5000 dilutions of this lot detected Histone H2A (acidic patch) in acid extracts from HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Histone H2A (acidic patch) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone H2A (acidic patch) Antibody Certificates of Analysis

      TitleLot Number
      Anti-Histone H2A (acidic patch) - 23561092356109
      Anti-Histone H2A (acidic patch) - 06080368360608036836
      Anti-Histone H2A (acidic patch) - 07010493830701049383
      Anti-Histone H2A (acidic patch) - 07010506480701050648
      Anti-Histone H2A (acidic patch) - 19707401970740
      Anti-Histone H2A (acidic patch) - 20173092017309
      Anti-Histone H2A (acidic patch) - 2017620176
      Anti-Histone H2A (acidic patch) - 20441832044183
      Anti-Histone H2A (acidic patch) - 21166242116624
      Anti-Histone H2A (acidic patch) - 22088162208816

      References

      Reference overviewApplicationSpeciesPub Med ID
      Critical Function of γH2A in S-Phase.
      Mejia-Ramirez, E; Limbo, O; Langerak, P; Russell, P
      PLoS genetics  11  e1005517  2015

      Show Abstract
      26368543 26368543
      Mutation of histone H3 serine 86 disrupts GATA factor Ams2 expression and precise chromosome segregation in fission yeast.
      Lim, KK; Ong, TY; Tan, YR; Yang, EG; Ren, B; Seah, KS; Yang, Z; Tan, TS; Dymock, BW; Chen, ES
      Scientific reports  5  14064  2015

      Show Abstract
      26369364 26369364
      Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation.
      Asghar, A; Lajeunesse, A; Dulla, K; Combes, G; Thebault, P; Nigg, EA; Elowe, S
      Nature communications  6  8364  2015

      Show Abstract
      26399325 26399325
      The EBNA3 family of Epstein-Barr virus nuclear proteins associates with the USP46/USP12 deubiquitination complexes to regulate lymphoblastoid cell line growth.
      Ohashi, M; Holthaus, AM; Calderwood, MA; Lai, CY; Krastins, B; Sarracino, D; Johannsen, E
      PLoS pathogens  11  e1004822  2015

      Show Abstract
      25855980 25855980
      Uncoupling transcription from covalent histone modification.
      Zhang, H; Gao, L; Anandhakumar, J; Gross, DS
      PLoS genetics  10  e1004202  2014

      Show Abstract
      24722509 24722509
      The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment.
      Yang, W; Lee, YH; Jones, AE; Woolnough, JL; Zhou, D; Dai, Q; Wu, Q; Giles, KE; Townes, TM; Wang, H
      Nature communications  5  3818  2014

      Show Abstract
      24784029 24784029
      Histone content increases in differentiating embryonic stem cells.
      Karnavas, T; Pintonello, L; Agresti, A; Bianchi, ME
      Frontiers in physiology  5  330  2014

      Show Abstract
      Western Blotting25221520 25221520
      The nucleosome acidic patch plays a critical role in RNF168-dependent ubiquitination of histone H2A.
      Mattiroli, F; Uckelmann, M; Sahtoe, DD; van Dijk, WJ; Sixma, TK
      Nature communications  5  3291  2014

      Show Abstract
      Western Blotting24518117 24518117
      Phosphorylation and arginine methylation mark histone H2A prior to deposition during Xenopus laevis development.
      Wang, WL; Anderson, LC; Nicklay, JJ; Chen, H; Gamble, MJ; Shabanowitz, J; Hunt, DF; Shechter, D
      Epigenetics & chromatin  7  22  2014

      Show Abstract
      Immunoblotting (Western)25302076 25302076
      Coordinate nuclear targeting of the FANCD2 and FANCI proteins via a FANCD2 nuclear localization signal.
      Boisvert, RA; Rego, MA; Azzinaro, PA; Mauro, M; Howlett, NG
      PloS one  8  e81387  2013

      Show Abstract
      Western BlottingHuman24278431 24278431

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies