ABE327 Anti-Histone H2A Antibody

ABE327
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IHC Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABE327
      DescriptionAnti-Histone H2A Antibody
      Alternate Names
      • Histone H2A type 1
      • H2A.1
      • Histone H2A/p
      Background InformationHistone H2A is one of four components of the core nucleosomal structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by the Tip60 enzyme which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues which mark mitosis and gene repression, and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A’s lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. It is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa acid extract and recombinant histone proteins
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-Histone H2A Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Histone H2A also known as Histone H2A type 1, H2A.1, Histone H2A/p.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected Histone H2A in liver and kidney tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Histone H2A at the C-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat. Broad species reactivity expected based on sequence similarity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H2AG
      • H2AFP
      • HIST1H2AI
      • H2AFC
      • HIST1H2AK
      • H2AFD
      • HIST1H2AL
      • H2AFI
      • HIST1H2AM
      • H2AFN
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~16 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract and recombinant histone proteins.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected Histone H2A on 10 µg of HeLa acid extract and recombinant histone proteins.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Histone H2A Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone H2A Antibody Certificates of Analysis

      TitleLot Number
      Anti-Histone H2A - 23883212388321
      Anti-Histone H2A - 24205352420535
      Anti-Histone H2A - 24615702461570
      Anti-Histone H2A - 21972862197286
      Anti-Histone H2A - 22763772276377
      Anti-Histone H2A - 23636052363605
      Anti-Histone H2A - 32293793229379
      Anti-Histone H2A - Q2013223Q2013223
      Anti-Histone H2A -25142302514230
      Anti-Histone H2A -27938532793853

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies