05-457 Anti-Histone H1 Antibody, clone AE-4

200 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, Pl ELISA, ICC, WB M Purified Monoclonal Antibody
      Catalogue Number05-457
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H1 Antibody, clone AE-4
      Alternate Names
      • H1 histone family, member 4
      • Histone H1b
      • histone 1
      • H1e
      • histone cluster 1
      • H1e
      Background InformationHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
      Product Information
      • Nuclear extracts from NIH3T3 or HeLa cells.
      PresentationPurified mouse monoclonal IgG2a in buffer containing PBS, pH 7.2, with 0.1% sodium azide. Frozen solution.
      ApplicationUse Anti-Histone H1 Antibody, clone AE-4 (mouse monoclonal antibody) validated in ELISA, ICC, WB to detect Histone H1 also known as H1 histone family member 4, Histone H1b, histone 1 H1e, histone cluster 1 H1e.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      5-20 µg/mL of a previous lot showed positive nuclear immunostaining in A431 cells fixed with 4% paraformaldehyde and methanol.

      Biological Information
      ImmunogenNuclei from myeloid leukemia biopsy cells. Clone AE-4.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes histone H1, Mr 32-33 kDa. Doublet detected due to hyperphosphorylation of histone H1 subpopulation. Cross-reacts with other histone isoforms in the following order of signal strength: H3, H2B > H4 > H2A, as determined with recombinant protein.
      Species Reactivity
      • Human
      • Bovine
      • Green Plants
      Species Reactivity NoteHuman and bovine. Predicted to stain nuclei from other animal and plant species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H1E
      • H1F4
      • H1.4
      • dJ221C16.5
      • MGC116819
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10412 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
      SIZE: 219 amino acids; 21865 Da
      SIMILARITY: SwissProt: P10412 ## Belongs to the histone H1/H5 family.
      MISCELLANEOUS: This variant accounts for 60% of histone H1.
      Molecular Weight32-33 kDa (Histone H1); Doublet (hyperphosphorylated Histone H1); 17 kDa (other histone isoforms).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on lysates from an acid extract of HeLa cells.

      Western Blot:
      1-4 µg/mL of this lot detected histone H1 in lysates from an acid extract of HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information


      Anti-Histone H1 Antibody, clone AE-4 SDS


      Safety Data Sheet (SDS) 

      Anti-Histone H1 Antibody, clone AE-4 Certificates of Analysis

      TitleLot Number
      Anti-Histone H1, clone AE-43068487
      Anti-Histone H1, clone AE-4 - 21133482113348
      Anti-Histone H1, clone AE-4 - 21555922155592
      Anti-Histone H1, clone AE-4 - 23834612383461
      Anti-Histone H1, clone AE-4 - 23874152387415
      Anti-Histone H1, clone AE-4 - 24203632420363
      Anti-Histone H1, clone AE-4 - 24284632428463
      Anti-Histone H1, clone AE-4 - DAM1734751DAM1734751
      Anti-Histone H1, clone AE-4 - 1626116261
      Anti-Histone H1, clone AE-4 - 1773517735


      Reference overviewApplicationPub Med ID
      Super-resolution microscopy reveals decondensed chromatin structure at transcription sites.
      Wang, Y; Maharana, S; Wang, MD; Shivashankar, GV
      Scientific reports  4  4477  2014

      Show Abstract
      24667378 24667378
      Deimination of linker histones links neutrophil extracellular trap release with autoantibodies in systemic autoimmunity.
      Dwivedi, N; Neeli, I; Schall, N; Wan, H; Desiderio, DM; Csernok, E; Thompson, PR; Dali, H; Briand, JP; Muller, S; Radic, M
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  28  2840-51  2014

      Show Abstract
      24671707 24671707
      The specification and global reprogramming of histone epigenetic marks during gamete formation and early embryo development in C. elegans.
      Samson, M; Jow, MM; Wong, CC; Fitzpatrick, C; Aslanian, A; Saucedo, I; Estrada, R; Ito, T; Park, SK; Yates, JR; Chu, DS
      PLoS genetics  e1004588  2014

      Show Abstract
      Immunofluorescence25299455 25299455
      Chromatin context and ncRNA highlight targets of MeCP2 in brain.
      Maxwell, SS; Pelka, GJ; Tam, PP; El-Osta, A
      RNA biology  10  1741-57  2013

      Show Abstract
      24270455 24270455
      Prostate cancer stem cell-targeted efficacy of a new-generation taxoid, SBT-1214 and novel polyenolic zinc-binding curcuminoid, CMC2.24.
      Botchkina, GI; Zuniga, ES; Rowehl, RH; Park, R; Bhalla, R; Bialkowska, AB; Johnson, F; Golub, LM; Zhang, Y; Ojima, I; Shroyer, KR
      PloS one  8  e69884  2013

      Show Abstract
      24086245 24086245
      High-resolution mapping of h1 linker histone variants in embryonic stem cells.
      Cao, K; Lailler, N; Zhang, Y; Kumar, A; Uppal, K; Liu, Z; Lee, EK; Wu, H; Medrzycki, M; Pan, C; Ho, PY; Cooper, GP; Dong, X; Bock, C; Bouhassira, EE; Fan, Y
      PLoS genetics  9  e1003417  2013

      Show Abstract
      23633960 23633960
      Novel alterations in the epigenetic signature of MeCP2-targeted promoters in lymphocytes of Rett syndrome patients.
      Lilja, T; Wallenborg, K; Björkman, K; Albåge, M; Eriksson, M; Lagercrantz, H; Rohdin, M; Hermanson, O
      Epigenetics  8  246-51  2013

      Show Abstract
      23348913 23348913
      Nicotine induces chromatin remodelling through decreases in the methyltransferases GLP, G9a, Setdb1 and levels of H3K9me2.
      Chase, KA; Sharma, RP
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  16  1129-38  2013

      Show Abstract
      23067581 23067581
      Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity.
      Cardinale, A; Racaniello, M; Saladini, S; De Chiara, G; Mollinari, C; de Stefano, MC; Pocchiari, M; Garaci, E; Merlo, D
      The Journal of biological chemistry  287  2618-31  2012

      Show Abstract
      22139836 22139836
      MicroRNA-195 plays a tumor-suppressor role in human glioblastoma cells by targeting signaling pathways involved in cellular proliferation and invasion.
      Zhang, QQ; Xu, H; Huang, MB; Ma, LM; Huang, QJ; Yao, Q; Zhou, H; Qu, LH
      Neuro-oncology  14  278-87  2012

      Show Abstract
      Western Blotting22217655 22217655

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